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- Table of Contents
EIF2AK4 · Western blot design guide
EIF2AK4 Western Blot Planning Guide
Plan a EIF2AK4 Western blot around the catalog-observed 186.9 kDa band, image-backed A01172-2 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
EIF2AK4 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 186.9 kDa observed | |
| Observed band | 186.9 kDa observed | |
| Gel | 8-10% | |
| Positive control | Cerebral cortex | |
| Negative control | Duodenum |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 186.9 kDa |
| ⓘ | Localization | Cytoplasm |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated EIF2AK4 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Cerebral cortex |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A01172-2 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected EIF2AK4 Western Blot Band Size?
Use the product-observed 186.9 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 186.9 kDa | Matches the authoritative product WB observation. |
| 186.9 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected EIF2AK4 appearancePlan around 186.9 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 186.9 kDa; use this as the primary experimental expectation. |
| Calculated mass | 186.9 kDa from UniProt Q9P2K8; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A01172-2. |
| Situation | Likely cause | Next action |
|---|---|---|
| 186.9 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing EIF2AK4 band
A01172-2 · EIF2AK4Observed 186.9 kDa · product-specific WB image
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for EIF2AK4 Western blot
🧪Use Cerebral cortex as the first positive-control candidate and retain an orthogonal negative control.
Positive control: Cerebral cortex (High)
Negative control: Duodenum (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for EIF2AK4
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cerebral cortex | Reported tissue cells | High | Protein (HPA) | HPA → |
| Lymph node | Reported tissue cells | High | Protein (HPA) | HPA → |
| Placenta | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced EIF2AK4 Western Blot Tips
Deeper troubleshooting and optimisation questions for EIF2AK4, answered from its protein features.
Which band should guide the blot?
Use 186.9 kDa, the observation attached to the authoritative A01172-2 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 186.9 kDa expectation.
Which positive control should I start with?
Start with Cerebral cortex, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Duodenum as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for EIF2AK4 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A01172-2 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
EIF2AK4 Western Blot Reagents
Human/Mouse/Rat-reactive EIF2AK4 Western blot reagents with authoritative product imagery.
Real WB data
Anti-EIF2AK4 Antibody Picoband®
Cat # A01172-2
Real WB data
Anti-GCN2/EIF2AK4 Antibody Picoband®
Cat # PA2028
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.