Product Info Summary
SKU: | PA2028 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-GCN2/EIF2AK4 Antibody
SKU/Catalog Number
PA2028
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GCN2/EIF2AK4 Antibody catalog # PA2028. Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GCN2/EIF2AK4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2028)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse GCN2, different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2028 is reactive to Eif2ak4 in Mouse, Rat
Applications
PA2028 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
94 kDa
Calculated molecular weight
186.487kDa
Background of GCN2
EIF2AK4 (Eukaryotic Translation Initiation Factor 2-Alpha Kinase 4), also called GCN2, is an enzyme that in humans is encoded by the EIF2AK4 gene. EIF2AK4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 to downregulate protein synthesis in response to varied cellular stresses. Hartz (2005) mapped the EIF2AK4 gene to chromosome 15q15.1 based on an alignment of the EIF2AK4 sequence with the genomic sequence. Berlanga et al. (1999) demonstrated that Gcn2 immunopurified from mouse liver extracts could phosphorylate rabbit Eif2 in vitro. Serum starvation increased the level of phosphorylated EIF2-alpha more than 2-fold in human embryonic kidney cells transfected with mouse Eif2ak4. Costa-Mattioli et al. (2005) reported a unique feature of hippocampal slices from Gcn2-null mice: in CA1, a single 100-Hz train induced a strong and sustained long-term potentiation (late LTP or L-LTP), which was dependent on transcription and translation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-GCN2 antibody, PA2028, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Mouse EIF2AK4, 35.3KD
(162aa tag+ E801-R954)
Lane 1: Recombinant Mouse EIF2AK4 Protein 10ng
Lane 2: Recombinant Mouse EIF2AK4 Protein 5ng
Lane 3: Recombinant Mouse EIF2AK4 Protein 2.5ng
Lane 4: Recombinant Mouse EIF2AK4 Protein 1.25ng
Protein Target Info & Infographic
Gene/Protein Information For Eif2ak4 (Source: Uniprot.org, NCBI)
Gene Name
Eif2ak4
Full Name
eIF-2-alpha kinase GCN2
Weight
186.487kDa
Superfamily
protein kinase superfamily
Alternative Names
EIF2AK4; eukaryotic translation initiation factor 2 alpha kinase 4; eukaryotic translation initiation factor 2-alpha kinase 4; GCN2; GCN2GCN2 eIF2alpha kinase; GCN2-like protein; KIAA1338EC 2.7.11.1 Eif2ak4|2610011M03, GCN, GCN2, MGCN2|eukaryotic translation initiation factor 2 alpha kinase 4|eIF-2-alpha kinase GCN2|GCN2 eIF2alpha kinase|GCN2-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Eif2ak4, check out the Eif2ak4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Eif2ak4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GCN2/EIF2AK4 Antibody (PA2028)
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No publications found for PA2028
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-GCN2/EIF2AK4 Antibody
Question
See attached the WB image, lot number and protocol we used for melanoma testis using anti-GCN2/EIF2AK4 antibody PA2028. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-03
Question
Is there a BSA free version of anti-GCN2/EIF2AK4 antibody PA2028 available?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GCN2/EIF2AK4 antibody PA2028 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-12
Question
I have a question about product PA2028, anti-GCN2/EIF2AK4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2028 anti-GCN2/EIF2AK4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-06
Question
We have observed staining in mouse melanoma testis. Are there any suggestions? Is anti-GCN2/EIF2AK4 antibody supposed to stain melanoma testis positively?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
Based on literature melanoma testis does express EIF2AK4. Based on Uniprot.org, EIF2AK4 is expressed in adenohypophysis, muscle uterus, brain, melanoma testis, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have EIF2AK4 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10718198
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Melanoma, and Testis, Pubmed ID: 17974005
Muscle, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-16
Question
Will anti-GCN2/EIF2AK4 antibody PA2028 work for WB with melanoma testis?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
According to the expression profile of melanoma testis, EIF2AK4 is highly expressed in melanoma testis. So, it is likely that anti-GCN2/EIF2AK4 antibody PA2028 will work for WB with melanoma testis.
Boster Scientific Support
Answered: 2019-11-28
Question
Will PA2028 anti-GCN2/EIF2AK4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
It shows on the product datasheet, PA2028 anti-GCN2/EIF2AK4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-30
Question
We are currently using anti-GCN2/EIF2AK4 antibody PA2028 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
The anti-GCN2/EIF2AK4 antibody (PA2028) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-21
Question
Our team were well pleased with the WB result of your anti-GCN2/EIF2AK4 antibody. However we have observed positive staining in melanoma testis cytoplasm. using this antibody. Is that expected? Could you tell me where is EIF2AK4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
According to literature, melanoma testis does express EIF2AK4. Generally EIF2AK4 expresses in cytoplasm. Regarding which tissues have EIF2AK4 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10718198
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Melanoma, and Testis, Pubmed ID: 17974005
Muscle, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-07
Question
Will anti-GCN2/EIF2AK4 antibody PA2028 work on pig WB with brain?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
Our lab technicians have not tested anti-GCN2/EIF2AK4 antibody PA2028 on pig. You can run a BLAST between pig and the immunogen sequence of anti-GCN2/EIF2AK4 antibody PA2028 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-13
Question
I see that the anti-GCN2/EIF2AK4 antibody PA2028 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-03
Question
I was wanting to use your anti-GCN2/EIF2AK4 antibody for WB for rat melanoma testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat melanoma testis identification?
Verified Customer
Verified customer
Asked: 2018-08-03
Answer
As indicated on the product datasheet, PA2028 anti-GCN2/EIF2AK4 antibody has been tested for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat melanoma testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-03
Question
Is a blocking peptide available for product anti-GCN2/EIF2AK4 antibody (PA2028)?
A. Li
Verified customer
Asked: 2018-02-19
Answer
We do provide the blocking peptide for product anti-GCN2/EIF2AK4 antibody (PA2028). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-02-19
Question
My question regards to test anti-GCN2/EIF2AK4 antibody PA2028 on rat melanoma testis for research purposes, then I may be interested in using anti-GCN2/EIF2AK4 antibody PA2028 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Collins
Verified customer
Asked: 2017-02-08
Answer
The products we sell, including anti-GCN2/EIF2AK4 antibody PA2028, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-02-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for melanoma testis using anti-GCN2/EIF2AK4 antibody PA2028. Let me know if you need anything else.
R. Taylor
Verified customer
Asked: 2013-11-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-11-22
Question
Is this PA2028 anti-GCN2/EIF2AK4 antibody reactive to the isotypes of EIF2AK4?
A. Zhang
Verified customer
Asked: 2013-03-14
Answer
The immunogen of PA2028 anti-GCN2/EIF2AK4 antibody is A synthetic peptide corresponding to a sequence in the middle region of mouse GCN2(868-886aa ATDHLAFTAEGKQDDQAGD), different from the related rat sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-03-14