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- Table of Contents
ELMO1 / Engulfment and cell motility protein 1 · Western blot design guide
Design a Western Blot for ELMO1
Source-linked ELMO1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-ELMO1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
ELMO1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~83.8 kDa | |
| Gel | 8–10% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 3 isoform(s) |
Section 1
Source-Linked ELMO1 Western Blot Protocol Options
The M03091-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Jurkat cell lysate (catalog M03091-1) |
| Gel % | 8–10% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | M03091-1; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected ELMO1 Western Blot Band Size?
ELMO1 is predicted at 83.8 kDa; isoforms could affect migration, but no empirical band or distinct isoform pattern is demonstrated.
| Band near 83.8 kDa | Consistent with the predicted ELMO1 sequence mass; confirm identity with controls |
| Two nearby bands | Isoforms 1, 2, and 3 are annotated, but their migration differences are unknown |
| Several bands at different positions | Alternative isoforms are possible contributors; band identities require validation |
| Weak band in a cytosolic fraction | ELMO1 can translocate to the cell membrane |
💡Expected ELMO1 appearanceELMO1 has a predicted mass of 83.8 kDa, but no empirical band size is supplied; confirm any candidate band with ordinary identity controls because isoform migration is unknown.
| Predicted ELMO1 mass | Full-length sequence predicts 83.8 kDa |
| Splice isoform 1 | Its individual mass and migration are not supplied |
| Splice isoform 2 | Its size relative to isoform 1 is not supplied |
| Splice isoform 3 | Its size relative to the other isoforms is not supplied |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | ELMO1 is cytoplasmic and membrane-associated; extraction or detection may be inadequate | Check extraction of both fractions and verify the antibody with a positive control |
| Band higher than expected | No listed feature establishes a higher apparent mass | Check antibody specificity with an independent antibody or ELMO1 depletion |
| Band lower than expected | Isoform masses are unknown, so a smaller band has uncertain identity | Compare an independent antibody or ELMO1 depletion |
| Multiple bands | Three splice isoforms are annotated, but distinct bands are unproven | Test band identity with isoform-aware controls or ELMO1 depletion |
| Weak or no signal | Membrane translocation may reduce ELMO1 in a cytosolic fraction | Examine the membrane fraction and a positive control |
Experiment evidence
Western blot images showing ELMO1 band
M03091-1 · ELMO1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for ELMO1 Western blot
🧪For positive controls for ELMO1 in Western blot, you can use an HPA-supported positive sample once one is identified; none is supplied here.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: ELMO1 is cytoplasmic and membrane-associated, but the supplied HPA data cannot establish suitable positive or negative tissues.
HPA tissue expression evidence for ELMO1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced ELMO1 Western Blot Tips
Deeper troubleshooting and optimisation questions for ELMO1, answered from its protein features.
How should ELMO1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
How might ELMO1 isoforms affect band interpretation?
Isoforms · Three isoforms are listed. Relative to the canonical sequence, isoform 2 lacks residues 1–480. Isoform 3 lacks residues 1–298 and replaces residues 299–362 with a different sequence. These differences could produce distinct bands; check which isoforms your antibody can detect before assigning them.
Determine whether the antibody recognizes sequence retained in each isoform. Isoform 2 lacks canonical residues 1–480; isoform 3 lacks 1–298 and replaces 299–362. Quantify only bands whose identity and antibody recognition are supported, and state whether the result represents one isoform or combined ELMO1 signal.
Determine whether the antibody recognizes sequence retained in each isoform. Isoform 2 lacks canonical residues 1–480; isoform 3 lacks 1–298 and replaces 299–362. Quantify only bands whose identity and antibody recognition are supported, and state whether the result represents one isoform or combined ELMO1 signal.
Which ELMO1 modifications are listed?
PTM · Canonical UniProt coordinates list HCK-linked phosphotyrosine at 18, 216, 395, 511, and 720; phosphoserine at 344; and N6-acetyllysine at 100 and 105. Match site-specific antibody labels to this coordinate convention before interpreting a signal.
Does this guide establish induction of ELMO1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for ELMO1?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M03091-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How can ELMO1 localization affect quantitation?
Quantitation · ELMO1 is listed in the cytoplasm and at the cell membrane, with plasma membrane translocation seemingly mediated by DOCK1 and CRK. For comparisons across samples, use the same lysate or fraction type and interpret changes in a membrane fraction alongside the corresponding cytoplasmic fraction.
Should ELMO1 run at its predicted 83.8 kDa?
Interpretation · 83.8 kDa is the predicted mass of canonical ELMO1. No observed band size is supplied. The listed modifications and isoforms may affect interpretation, but their presence alone does not establish a visible shift or explain a mass difference.
HCK is listed for phosphorylation of canonical ELMO1 Tyr18, Tyr216, Tyr395, Tyr511, and Tyr720. A site-specific phosphotyrosine antibody could assess the corresponding signal alongside total ELMO1. The feature list does not establish when phosphorylation occurs or whether it changes migration.
Compare candidate bands with the three listed isoforms and the antibody's target sequence. Canonical sites 18, 100, 105, 216, 344, 395, 511, and 720 need careful mapping to alternative sequences: isoform 2 lacks 1–480, while isoform 3 lacks 1–298 and replaces 299–362. Neither feature presence nor a band alone establishes its identity.
HCK is listed for phosphorylation of canonical ELMO1 Tyr18, Tyr216, Tyr395, Tyr511, and Tyr720. A site-specific phosphotyrosine antibody could assess the corresponding signal alongside total ELMO1. The feature list does not establish when phosphorylation occurs or whether it changes migration.
Compare candidate bands with the three listed isoforms and the antibody's target sequence. Canonical sites 18, 100, 105, 216, 344, 395, 511, and 720 need careful mapping to alternative sequences: isoform 2 lacks 1–480, while isoform 3 lacks 1–298 and replaces 299–362. Neither feature presence nor a band alone establishes its identity.
Boster reagents
ELMO1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-ELMO1 Rabbit Monoclonal Antibody
Cat # M03091-1
The catalog reports one anti-ELMO1 antibody, M03091-1, a rabbit monoclonal with stated human, mouse, and rat reactivity. Its Western blot image shows ELMO1 expression in Jurkat cell lysate; the supplied evidence does not show mouse or rat samples.
Which to pick: M03091-1 is the only listed option and has a Western blot image using Jurkat cell lysate. Check its stated species reactivity against your sample; the supplied image documents the Jurkat context only.
Source: BosterBio ELMO1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q92556.
- Human Protein Atlas. ELMO1 tissue expression.
- PMC12629339 — target-verified WB comparison
- PMC9899619 — target-verified WB comparison