EXOSC2 · Western blot design guide

EXOSC2 Western Blot Planning Guide

Plan an EXOSC2 Western blot around the catalog-observed 32.8 kDa band, image-backed A10119-1 evidence, HPA controls, and verified protocol records.

Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for EXOSC2 (EXOSC2): expected band 32.8 kDa, antibody A10119-1, and PMC-cited SDS-PAGE protocol steps

Band Protocols Controls Antibodies

EXOSC2 Western Blot Experimental Design Guide

Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	32.8 kDa
	Observed band	Not reported — verify product WB image
	Gel	12-15%
	Positive control ⓘ	Cerebellum
	Negative control ⓘ	Target knockdown/knockout

Important caveats

Reasons your observed band may differ from the expected size.

ⓘ	Calculated mass	32.8 kDa
ⓘ	Localization	Cytoplasm / Nucleus, nucleolus
ⓘ	Processing / PTM	Record-dependent
ⓘ	Reactivity	Human

Sample controls for EXOSC2 Western blot

🧪Use Cerebellum as the first positive-control candidate; no defensible HPA Not detected tissue was available, so use a target knockdown/knockout negative control.

Positive control: Cerebellum (High)

Negative control: Target knockdown/knockout

HPA protein score determines control status; other expression data is supporting context only.

HPA tissue expression evidence for EXOSC2

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.

Positive expression · recommended positive controls

Tissue	Cell type	Level	Evidence	Source
Cerebellum	Reported tissue cells	High	Protein (HPA)	HPA →
Gallbladder	Reported tissue cells	High	Protein (HPA)	HPA →
Hippocampus	Reported tissue cells	High	Protein (HPA)	HPA →
Cerebral cortex	Reported tissue cells	High	Protein (HPA)	HPA →

Undetected expression · recommended negative controls

Tissue	Cell type	Level	Evidence	Source

No HPA Not detected tissue is available; use a target knockdown/knockout negative control.

Section 1

What Is the Expected EXOSC2 Western Blot Band Size?

Use the product-observed 32.8 kDa band as the primary planning value and retain the UniProt calculated mass as context.

What am I looking at on my blot?
32.8 kDa	Matches the authoritative product WB observation.
32.8 kDa calculated	Use as UniProt context, not as a replacement observed band.
Unexpected additional signal	Do not assign identity without orthogonal positive/negative controls.

💡Expected EXOSC2 appearancePlan around 32.8 kDa and keep the calculated mass as supporting context.

How each factor affects band size
Catalog-observed band	32.8 kDa; use this as the primary experimental expectation.
Calculated mass	32.8 kDa from UniProt Q13868; retain as context.
Gel selection	12-15%; shared with the recommended protocol and poster.
Specificity check	Compare the lead HPA positive and negative controls with A10119-1.

Why is my band missing or off?
Situation	Likely cause	Next action
32.8 kDa	Matches the authoritative product WB observation.	Confirm with orthogonal controls and the linked product record.
Additional band	May reflect processing, modification, or non-specific signal.	Run a dilution series and compare positive/negative controls.
Weak signal	Target abundance or transfer may be limiting.	Verify transfer, increase positive-control abundance, and bracket exposure.

Sources: UniProt Q13868 Human Protein Atlas product datasheet (A10119-1)

Experiment evidence

Western blot images showing EXOSC2 band

A10119-1 · EXOSC2 — observed 32.8 kDa WB validation

A10119-1 · EXOSC2Observed 32.8 kDa · product-specific WB image

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Section 2

Real Curated EXOSC2 Western Blot Protocols

Start with the molecular-weight rule, then compare verified publication-derived conditions.

Recommended Western blot protocol parameters
Sample / lysate	Cerebellum
Gel %	12-15%
Load	20-30 µg total protein per lane
Transfer	Semi-dry, standard transfer
Membrane	0.45 µm PVDF
Blocking	5% non-fat milk or 5% BSA in TBST
Primary	A10119-1 at datasheet starting dilution
Primary incubation	Overnight at 4 °C with gentle agitation
Secondary	Species-matched HRP conjugate at validated dilution
Wash	3 × 5 min in TBST
Detection	Chemiluminescent substrate
Exposure	Bracket exposures to avoid saturation

Section 4

Advanced EXOSC2 Western Blot Tips

Deeper troubleshooting and optimisation questions for EXOSC2, answered from its protein features.

Which band should guide the blot?

Use 32.8 kDa, the observation attached to the authoritative A10119-1 WB record.

How should calculated mass be interpreted?

Treat the UniProt calculated mass as context; it does not replace the catalog-observed 32.8 kDa expectation.

Which positive control should I start with?

Start with Cerebellum, the lead HPA protein-expression candidate.

Which negative control is defensible?

Use a target knockdown/knockout control when no HPA Not detected tissue is available.

Which gel should I use?

Use 12-15% consistently across the quick facts, protocol table, and poster.

What transfer method to use for EXOSC2 Western blot?

Use the transfer method in the recommended protocol and verify transfer before blocking.

How should A10119-1 be started?

Start at the linked datasheet condition and run a three-point primary-antibody dilution test.

Which publication-derived protocols can I compare?

Compare only the supplied target-verified records: PMC11358264, PMC9585911, PMC6403362.

Boster reagents

EXOSC2 Western Blot Reagents

Human-reactive EXOSC2 Western blot reagents with authoritative product imagery.

Real WB data Western blot validation image for EXOSC2 using A10119-1; observed band 32.8 kDa

Anti-RRP4/EXOSC2 Antibody Picoband®

Cat # A10119-1

Reactivity: Human

View product →

Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.

Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.