FANCC / Fanconi anemia group C protein · Western blot design guide

Design a Western Blot for FANCC

Real validated FANCC Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-FANCC WB antibodies. Everything you need to plan the experiment before you commit precious samples.

Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for FANCC: expected band ~63.4 kDa, hero antibody A02387-1, catalog values and labelled standard workflow; separate PMC comparisons on the guide — Printable **FANCC** Western blot protocol sheet — expected band ~63.4 kDa, antibody A02387-1, controls and PMC citations. Open the full FANCC WB guide →

Navigate to: Protocols Band Controls Antibodies

FANCC Western Blot Experimental Design Guide

Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	~63.4 kDa
	Gel	12–15% (standard starting point)
	Positive control ⓘ	Testis (IHC candidate; verify WB) +4 more
	Negative control ⓘ	Adipose tissue (IHC candidate; verify WB)

Important caveats

Reasons your observed band may differ from the expected size.

	PTM	—
	Caveat	—
	Gene-set association	MSigDB Hallmark membership
	Isoform	1 isoform(s)

Section 1

Real Curated FANCC Western Blot Protocols

The A02387-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.

Recommended Western blot protocol parameters
Sample / lysate	Target-positive lysate and matched negative control (standard starting point)
Gel %	12–15% (standard starting point)
Load	20–30 µg total protein per lane; optimize for abundance (standard starting point)
Transfer	Standard semi-dry transfer; verify efficiency (standard starting point)
Membrane	0.45 µm PVDF (standard starting point)
Blocking	5% milk or 5% BSA in TBST (standard starting point)
Primary antibody	A02387-1; use the WB datasheet starting dilution (standard starting point)
Primary incubation	Overnight at 4 °C (standard starting point)
Secondary antibody	goat anti-rabbit IgG, 1:5000 (catalog A02387-1)
Secondary incubation	1 h at room temperature (standard starting point)
Wash	3 × 5 min in TBST (standard starting point)
Detection	ECL; bracket exposures to avoid saturation (standard starting point)

Section 2

What Is the Expected FANCC Western Blot Band Size?

FANCC is predicted at 63.4 kDa; no empirical band size is supplied, and its listed features do not demonstrate altered migration.

What am I looking at on my blot?
Single band near 63.4 kDa	Consistent with full-length FANCC
Band near 63.4 kDa in whole-cell lysate	Consistent with cellular FANCC
Band near 63.4 kDa in a nuclear fraction	Consistent with FANCC's major nuclear pool
Band near 63.4 kDa in a cytoplasmic fraction	Consistent with FANCC's minor cytoplasmic pool

💡Expected FANCC appearanceUniProt predicts 63.4 kDa for FANCC; no empirical band size is supplied, so confirm a band near that size using FANCC depletion and an independent antibody.

How each factor affects band size
UniProt predicted molecular weight	Places full-length FANCC near 63.4 kDa
Predicted mass in daltons	63,429 Da corresponds to approximately 63.4 kDa
Predicted full-length band position	Provides a reference near 63.4 kDa, without measured migration
Predicted mass for band comparison	Does not establish the position of an empirical band

Why is my band missing or off?
Situation	Likely cause	Next action
No band in lysate	Predominantly nuclear FANCC may be poorly recovered during extraction	Check nuclear protein recovery and compare a nuclear fraction
Band higher than expected	The supplied features do not establish a size-increasing modification	Verify band identity by FANCC depletion and an independent antibody
Band lower than expected	The supplied features do not establish a cleaved FANCC product	Check sample integrity and whether the band decreases with FANCC depletion
Multiple bands	No additional FANCC isoforms or size-changing modifications are listed	Compare bands after FANCC depletion and with an independent antibody
Weak or no signal	FANCC may be underrepresented in the tested fraction	Check nuclear extraction and include a suitable positive lysate

Source: UniProt Q00597 Human Protein Atlas product datasheet (A02387-1)

Experiment evidence

Western blot images showing FANCC band

A02387-1 · FANCC

A02387-2 · FANCC

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Sample controls for FANCC Western blot

🧪HPA-IHC candidate guidance (verify in WB): For positive controls for FANCC in Western blot, you can use testis lysate, which has high HPA expression.

Positive control: Testis (IHC candidate; verify WB)

Negative control: Adipose tissue (IHC candidate; verify WB)

Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.

⚠️Feasibility: A clean tissue negative is available, and nuclear enrichment may help detect predominantly nuclear FANCC.

HPA tissue expression evidence for FANCC

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.

Higher expression tissues · candidate positive controls from IHC

Tissue	Cell type	Level	Evidence	Source
Testis	spermatogonia cells	High	Protein (IHC)	HPA →
Adrenal gland	glandular cells	Medium	Protein (IHC)	HPA →
Appendix	glandular cells	Medium	Protein (IHC)	HPA →
Bone marrow	hematopoietic cells	Medium	Protein (IHC)	HPA →
Breast	glandular cells	Medium	Protein (IHC)	HPA →

Lower expression tissues · IHC evidence, not confirmed WB-negative controls

Tissue	Cell type	Level	Evidence	Source
Adipose tissue	adipocytes	Not detected	Protein (IHC)	HPA →
Lymph node	germinal center cells	Not detected	Protein (IHC)	HPA →
Spleen	cells in red pulp	Not detected	Protein (IHC)	HPA →
Caudate	glial cells	Low	Protein (IHC)	HPA →
Epididymis	glandular cells	Low	Protein (IHC)	HPA →

Section 3

Advanced FANCC Western Blot Tips

Deeper troubleshooting and optimisation questions for FANCC, answered from its protein features.

Where should the main FANCC band appear?

Band shift · The predicted mass is 63.4 kDa. Use that as a reference when identifying FANCC, but the supplied features provide no observed band position, so they cannot establish its apparent mass on a blot.

Could annotated isoforms explain multiple FANCC bands?

Isoforms · UniProt lists one FANCC isoform and no alternative sequence. The supplied features therefore do not support assigning additional bands to annotated isoforms.

Do annotated modifications predict a FANCC band shift?

PTM · No modified residues or glycosylation sites are listed. The supplied features do not predict a modification-related shift or explain any difference between apparent and predicted mass.

Does this guide establish induction of FANCC?

Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.

How should transfer be checked for FANCC?

Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.

How should blocking be optimized?

Blocking · Standard workflow guidance: follow the A02387-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.

How should FANCC be quantified across fractions?

Quantitation · Quantify nuclear and cytoplasmic fractions separately. Because the major FANCC form is nuclear, a whole-cell measurement can obscure a change confined to one compartment.

Which fraction should contain most FANCC?

Interpretation · FANCC is found in the nucleus and cytoplasm, with the major form nuclear and the minor form cytoplasmic. Expect stronger FANCC signal in a nuclear fraction if the fractions are comparable.

FANCC is associated with DNA damage and repair, but the supplied features do not specify an induction condition or predict increased protein abundance. Interpret any signal change against the experiment's controls.

Compare them with the 63.4 kDa predicted mass and check nuclear versus cytoplasmic fractions. FANCC belongs to a multisubunit FA complex, but these features do not establish that complex membership produces a particular extra band.

Boster reagents

FANCC Western Blot Antibodies

Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.

Real WB data Western blot analysis of lysates from HepG2,HL-60 cell line (from left to right),using FANCC Antibody (C-term).A02387-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.

Western blot analysis of lysates from HepG2,HL-60 cell line (from left to right),using FANCC Antibody (C-term).A02387-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.

Anti-FANCC Antibody (C-term)

Cat # A02387-1

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Real WB data

Western blot analysis of FANCC using anti-FANCC antibody (A02387-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FANCC antigen affinity purified polyclonal antibody (Catalog # A02387-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FANCC at approximately 63 kDa. The expected band size for FANCC is at 63 kDa.

Anti-FANCC Antibody Picoband®

Cat # A02387-2

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The catalog reports two human-reactive anti-FANCC antibodies with WB images. A02387-1 was shown with HepG2 and HL-60 lysates; A02387-2 with HeLa, 293T, HaCaT, and A431 lysates, with a reported band near 63 kDa. These examples do not establish performance across all human samples.

Which to pick: For a documented 63 kDa band and detailed WB conditions, consider A02387-2. For HepG2 or HL-60 lysates, A02387-1 has a WB example at 1:1000. Choose based on your sample and the reported conditions.

Source: BosterBio FANCC gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.

References

UniProt Consortium. UniProt entry Q00597.
Human Protein Atlas. FANCC tissue expression.
PMC4605623 — target-verified WB comparison
PMC3968024 — target-verified WB comparison