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1 Q&As
Facts about Flap endonuclease 1.
It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap.
Human | |
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Gene Name: | FEN1 |
Uniprot: | P39748 |
Entrez: | 2237 |
Belongs to: |
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XPG/RAD2 endonuclease family |
EC 3.1; FEN1; FEN-1; FEN-1hFEN-1; flap structure-specific endonuclease 1DNase IV; Maturation factor 1; maturation factor-1; MF1flap endonuclease 1; RAD2
Mass (kDA):
42.593 kDA
Human | |
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Location: | 11q12.2 |
Sequence: | 11; NC_000011.10 (61792911..61797238) |
[Isoform 1]: Nucleus, nucleolus. Nucleus, nucleoplasm. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; [Isoform FENMIT]: Mitochondrion.
Boster Bio may be a product that you have heard about and wondered how it might help you. If you're looking for an FEN1 marker that is reliable, read this article and learn more about its functions and applications. The product can also be used to earn product credits for sharing your results. Boster Bio's FEN1 human protein is expressed in E. coli. It has an aa-sized sequence domain that is 1-380 aa.
FEN1 is a 42 kDa protein that acts as an exonuclease and as an endonuclease. FEN1 is a vital component of the replication of DNA in cells. Although it isn't approved for human use, FEN1 plays a critical role in DNA replication. FEN1 plays many important roles but is not approved as a treatment or therapeutic antibody.
Flap endonuclease (or FEN1) is a enzyme for cDNA-synthesis that cleaves DNA flaps of up to 200 nucleotides in length. This protein is structure-specific and is able to assume high- or low-activity conformational state. FEN1 evolved to function as complexes to achieve its function. It would not be harmful when it was acting on its own.
When FEN1 is used as a marker for the genome can be used by researchers to identify specific DNA fragments. FEN1 cleaves a flap in the downstream region. The enzyme is inhibited by a secondary structure at the flap that is 5. This secondary structure can be created by annealing an Oligonucleotide. The enzyme then proceeds to create a nick in the downstream region of the DNA strand.
In addition to being a reliable biomarker, FEN1 can also be utilized as a target to aid in the development of chemotherapeutics. As demonstrated in Nikolova et al. the downregulation of FEN1 decreased sensitivity to CDDP and improved sensitivity to methylating agents. It has also been shown that it increases the susceptibility to alkylating agents. FEN1 downregulation may cause nucleotide excision damage which is a major target for platinum-based chemotherapy.
The FEN1 gene has been studied extensively in the field of cancer research. Researchers have utilized FEN1 to discover mutations that are associated with cancer risk. Researchers believe that the gene is involved in tumor suppression. However, further studies are required to determine the exact mechanism by which FEN1 functions in the human body. The FEN1 gene is a key biomarker for lung cancer , as well as digestive disorders.
FEN1 is an old protein with multiple roles in the process of metabolism of DNA. Over the course of time FEN1's particular and efficient form has been refined. Its unique ability helps with genome stability and cell development and growth. Additionally, FEN1 interacts with several other proteins. Numerous high-profile FEN1 studies have been conducted on humans.
The gene known as flap endonuclease (FEN1) plays multiple roles in DNA replication and repair. This gene is highly regarded as therapeutic target due to its role in maintaining healthy DNA and the replication of the genome. This gene has been identified as a possible target for cancer therapy. This study provides further details about FEN1 and its potential as an effective therapeutic target.
FEN1 mRNA, and FEN1 protein were identified as being overexpressed in HCC cohorts. The FEN1 gene expression in these tumors was negatively associated with poor survival. Hence, the FEN1 gene is a likely candidate for predicting the prognosis of patients with HCC. But what does this mean? Functional enrichment analysis, Kaplan Meier analysis, and pathway enrichment analysis identified potential biological functions and molecular mechanism of FEN1 genes.
The FEN1 gene is present in a variety of malignant tumors. This gene may increase tumor aggressiveness by being overexpressed. This finding has been confirmed in HCC by tissue microarray and immunohistochemistry. In the same study, FEN1 expression was also detected in 396 patients with HCC. This is consistent with recent research that has demonstrated the function of FEN1 in cancer cells. For instance in the case of breast cancer, FEN1 expression can predict the severity of the disease.
The functions of the FEN1 marker are complex. The transcription factor YY1, which regulates FEN1 levels when DNA-damaging agents are applied is responsible for regulating the gene. FEN1 levels are also elevated in breast cancer. This is because they are directly connected to the degree of cancer drug resistant. The gene could play a crucial role in the prevention of breast cancer and its progression.
The FEN1 marker has many applications. It is used to evaluate prostate cancer, non-small-cell lung cancer and kidney cancer. It can also be combined with other markers to assess cancer. Below are a few examples of these applications. Let's take a look at the other. To begin, FEN1 is widely used as a cancer marker. Its elevated levels can indicate whether a sample is positive for cancer or not.
Molecular markers are a crucial element in screening, diagnosis, prognosis, and treatment. These tests can be made using the FEN1 marker. Multi-marker panel panels can improve diagnostic accuracy more than the use of a single marker. FEN1 can also be used to create markers panels for certain kinds like EC and BC. Additionally, the FEN1 marker is a way to measure the risk of lung cancer in patients with CC or BC.
Antibodies raised against the FEN1 protein are suitable for analysis using quantitative methods. They don't need to be specific to FEN1 because any skilled craftsman can determine the conditions that enable the formation of. Monoclonal antibodies are known to have properties that are constant, which make them an ideal instrument for the development of immunoassays for routine clinical use. In this article, we will discuss the creation and use of monoclonal antibody against FEN1.
The FEN1 gene is located on human 11th chromosome. It also performs various functions associated with DNA metabolism. It is responsible for getting rid of the 5'-overhanging flap that is the result of displacement synthesis. This happens when a DNA polymerase encounters an Okazaki fragment downstream. FEN1 also is a part of RNase H activity. Both of these factors influence FEN1's actions.
Research has revealed that the FEN1 molecule could be useful in detecting the presence of cancer cells. Interestingly, FEN1 is essential for the growth of many cancer cells. In the study published in 2015 in the journal Cell, the FEN1 gene was linked with a heightened sensitivity to Temozolomide. However, despite the heightened FEN1 sensitivity there has been no consensus regarding the safety of this marker. There have been various types of cancers linked to FEN1 gene, including pancreatic, gastric and lung cancers and neuroblastoma.
Serum levels of FEN1 and CEA were determined using the ELISA Kit. FEN1 was a better diagnostic marker than CEA and CA153 in BC however the combined measurement had no higher diagnostic parameters than a single measurement. This study suggests that FEN1 should not be used solely as a diagnostic instrument. It can be useful in the detection of BC, and may be a complement to screening for cancer.
Researchers discovered that FEN1 expression was higher in stage II breast cancer patients than in patients with grade I. However only a handful of patients showed a correlation Ki-67 status. Studies on the function of FEN1 are needed to confirm the role of FEN1 in DCIS prognosis. The study authors concluded that FEN1 gene expression could improve the survival of patients.
FEN1 is a crucial component of genome fidelity. However, recent advances in understanding its role suggest that it might be an ancient protein. It has been fine-tuned over the course of eons of evolution, and coordinates a variety of vital DNA transactions. FEN1 interacts with WRN even when replication is stalled. It is not a cancer-causing agent but the research has shown that it can be utilized in cancer treatment.
PMID: 8007985 by Murray J.M., et al. Structural and functional conservation of the human homolog of the Schizosaccharomyces pombe rad2 gene, which is required for chromosome segregation and recovery from DNA damage.
PMID: 7774922 by Hiraoka L.R., et al. Sequence of human FEN-1, a structure-specific endonuclease, and chromosomal localization of the gene (FEN1) in mouse and human.