FLI1 Antibodies

Friend leukemia integration 1 transcription factor (FLI1)

Sequence-specific transcriptional activator (PubMed:24100448, PubMed:26316623, PubMed:28255014). Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

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Gene Information

Human
Gene Name:	FLI1
Uniprot:	Q01543
Entrez:	2313

Family

Belongs to:
ETS family

Alternative Names

ERGB; EWSR2; FLI1; Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; Proto-oncogene Fli-1; SIC-1; Transcription factor ERGB

Molecular Weight

Mass (kDA):
50.982 kDA

Genomic Context

Human
Location:	11q24.3
Sequence:	11; NC_000011.10 (128685263..128813267)

Subcellular Localizations

Nucleus.

Diseases

• Neoplasms
• Ewings Sarcoma-primitive Neuroectodermal Tumor (pnet)
• Sarcoma
• Leukemia
• Chromosomal Translocation
• Neuroectodermal Tumors
• Malignant Neoplasms
• Bone Neoplasms
• Neuroectodermal Tumor, Primitive
• Cell Transformation, Neoplastic
• T(11;22)(q24;q12)
• Malignant Paraganglionic Neoplasm

• Tumor Angiogenesis
• Acute Erythroblastic Leukemia
• Neoplasm Metastasis
• Myeloid Leukemia
• Rhabdomyosarcoma
• Cytogenetic Abnormality
• Leukemia, Myelocytic, Acute

Interacting Proteins

• SIAH1
• SOX30

Pathways

• Angiogenesis
• Pathogenesis
• Oncogenesis
• Cell Proliferation
• Cell Differentiation
• Localization
• Rna Interference
• Cell Cycle
• Vasculogenesis
• Cell Growth
• Interphase
• Wound Healing
• Cell Development

• Cell Migration
• Translation
• Cell Death
• Cell Adhesion
• Regeneration
• Metaphase
• Blood Circulation

PTMs

• Phosphorylation
• Acetylation
• Methylation
• Cleavage

• Ubiquitination
• Phosphorothioation
• Sumoylation
• Deacetylation

• Dephosphorylation

Research Areas

• Cancer
• Tumor Suppressors

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/FLI1

Boster Bio - Best Uses Of The FLI1 Marker

If you're not acquainted with flow the cytometry method, you're probably wondering what this molecule is and how it's used in science. The short answer is a range of applications. The technique is used to analyze particles or cells. Boster provides polyclonal and monoclonal antibody with high affinity. Boster antibodies have been featured in publications and continue to be cited for 25 years!

ELISA

An ELISA that uses Boster Bio's FlI1 marker allows for rapid and efficient detection of FLI1 expression in cultured cells. With this kit, researchers can measure the amount of FLI1 in cells that have been cultured, and examine the effects of different treatments or activators on the expression of FLI1.

The Fli-1 protein is found in 74% of ovarian cancers and is up-regulated in EOC tissues. A high level of Fli-1 expression is associated with a lower overall survival and a lower chance of developing the disease. This makes it a desirable therapeutic option. Furthermore, this marker was discovered to correlate with other clinical variables like the stage of cancer and lymph node involvement. However, larger multicentric studies are needed to validate these findings.

When determining the expression of Fli-1 within cells, an ELISA test using Boster Bio's marker can to identify cells that are overexpressed in tumors. This test can be used to monitor the expression of tumor cells within the blood. Additionally, it permits scientists to submit their results for species-specific research. Boster scientists can also receive product credits for their research results. All scientists can utilize the ELISA method.

Utilizing a Rosetta gami Extract Boster Bio's marker used to conduct ELISA. The sample was incubated overnight at 4degC. The supernatant then was collected and kept at -20°C until use. A 96-well ELISA plate that contained 200 ml overnight culture was pre-coated with 2 mg/ml of mCD99 protein. Next, the bacteria's pellet was sonicated 15 cycles of 20 hours on and 30 hours off. The final lysate was centrifuged at 4degC, and stored at 20degC.

WB

Recently, the FLI1 marker was detected in human intestinal metaplasia epithelial cell lines. This marker can be used to identify various cancers. The development of embryos and maintenance of blood vessels are also affected by the FLI1 gene. However it was not discovered in nonvascular tumors. These results highlight the advantages of the FLI1 gene in cancer diagnosis. Let's take a closer look at the most popular uses of FLI1.

Gastric cancers typically show a decrease in FLI1 expression. It also reduces the invasiveness and proliferation of tumors, FLI1 is thought to play a role in the transition from pre-cancer lesions into aggressive adenocarcinomas. FLI1 could be an important biomarker for the different subgroups of gastric cancer, and its clinical significance is dependent on the specific function it plays in the development of the cancer.

IHC

The FLI1 gene encodes an expression domain for an transcription factor that contains DNA-binding elements. FLI1 is a protooncogene that is expressed in the majority of erythroleukemias caused by F-MuLV. FLI1 is a constitutively activated protein in erythroblasts, causing an abrupt shift in the Epo/EpoR signaling.

Boster Bio's anti-FLI1 antibody interacts with mouse, human rat, and mouse cells. The antibody is contained in a lyophilic tube containing trehalose and Na2HPO4 and is suitable for use in IHC. Boster Bio AntiFLI1 Picoband (A00399) can be used in IHC experiments.

This antibody is designed to recognize all five human CD99 isoforms. It uses primer pairs of l and k + to identify variants 1, 5 and 6. The full-length CD99 isoform can be identified by the a +/b primer pair. Primer pairs such as d+ e and F+-e detect the truncated version. These antibodies are highly sensitive and specific.

Human CD99 expression can be detected in activated endothelial cells. The cells that are activated express the CD99 short isoform. The 16 kDa bands indicate that there is a different form. The beginning of an exon is represented by the alanine band , which is dark blue. If there is a distinction between exons, it's a sign of a different variant.

ICC

Clinical applications of the FLI1 marker in the treatment of ovarian cancer are possible through ICC. Fli-1 is a nuclear marker for endothelial division. Fli-1 is a nuclear marker of endothelial differentiation. It is able to be knocked down in ovarian cancer cells however it does not influence metastasis. While Fli-1 could be important in the development of ovarian cancer it is unclear what makes it a valuable marker.

The vascular system plays a crucial role for fetal growth. There are many variables that influence the strength and growth of the vascular system. Fli1 is one of them. Fli1 is a member in the Ets family plays a critical role in the vascular morphogenesis process at mid-gestation. Fli1 expression is stopped at mid-gestation. The mechanisms that drive its action are not yet understood. This article will discuss some of these possible uses of the FLI1 marker.

Fli1 is expressed within the vascular system inside MPCs. It regulates the endothelial versus myogenic potential of MPCs. It is important to remember that Fli1 expression is not found in skeletal myofibers. MPCs with Fli1 expression showed an increase in endothelial gene expression and attenuated myogenic gene expression. This effect was partially reversed by the forced expression of myogenic regulators. In addition, MPCs that express Fli1 showed higher expression in X-linked muscular dystrophic mice.

The expression of Fli-1 is a new marker for ovarian carcinoma. It also has therapeutic potential. It is a potential target for chemotherapy. It stimulates angiogenesis, which is the process through which cells divide , and eventually become cancerous. The protein may also play an important role in the treatment of cancer. The ICC uses of the FLI1 marker

Immunofluorescence

Boster Bio is a manufacturer of antibodies against FLI1. We offer a variety of anti-FLI1 products. The antibodies have been validated on various platforms using positive and negative samples. Boster also awards credits to the first reviewer to their products. This reward program is accessible to all scientists from around the world. The FLI1 marker can be used to examine the immune system in different ways. It is especially useful in the field of cancer research as well as in identifying lymphocytes and granulocytes.

The FLI1 protein, which is part of the ETS family of DNA binding transcription factors, is involved in the proliferation of cells and tumorigenesis. According to Ewing's sarcomas around 90% have a translocation of FLI1 genes. By fusing EWS with FLI-1, the protein EWS-Fli-1 is produced. An antibody against FLI-1 can be obtained to detect the protein in various types of vascular tumors.

Numerous studies have proven that Fli1 expression alters the erythroblast's response to Epo, leading to abnormal proliferation. FLI1 overexpression can also alter the signaling processes that are associated to terminal differentiation and proliferation, which can lead to cell death. This mutation is responsible for the high prevalence of erythroleukemia induced by F-MuLV. The FLI1 marker has also demonstrated positive correlations with poor outcomes in cancer.

When using the FLI1 marker it is crucial to note that it is not just useful for cancer research, but also useful in other fields. The light-emitting signal it emits is caused due to compounds found in cells. Larger cells produce more fluorescent compounds, and have higher levels of autofluorescence. It is important to use unstained control cell or FMO controls to determine the kind of cell. This will avoid false positives.