This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
5 Citations 1 Q&As
5 Citations 7 Q&As
Facts about Follicle-stimulating hormone receptor.
.
Human | |
---|---|
Gene Name: | FSHR |
Uniprot: | P23945 |
Entrez: | 2492 |
Belongs to: |
---|
G-protein coupled receptor 1 family |
follicle stimulating hormone receptor; follicle-stimulating hormone receptor; Follitropin receptor; FSH R; FSH receptor; FSHR; FSH-R; LGR1; LGR1FSHRO; MGC141667; MGC141668; ODG1
Mass (kDA):
78.265 kDA
Human | |
---|---|
Location: | 2p16.3 |
Sequence: | 2; NC_000002.12 (48953161..49154527, complement) |
Sertoli cells and ovarian granulosa cells.
Cell membrane; Multi-pass membrane protein.
The FSHR is transmembrane receptor that binds with follicle-stimulating hormone (FSH). It is a key component in steroidogenesis, and could be utilized as a target for steroid production. Boster Bio offers several products to detect this transmembrane. Read on for more information. Boster Bio: Best Uses of the FSHR Marker
The FSHR is a transmembranous protein that is similar to the endothelial cell in blood vessels. This protein is present in tumors of the kidney colon, ovary, bladder, and testis as well as in microvessels and in the brain. The protein is also found in blood cells that circulate. A cancer patient who is FSHR positive will most likely suffer from a cancer-related disease.
The FSHR protein is believed to contain seven transmembrane segments as well as a 7-helical additionalcellular region. The receptor protein is 90% similar to its homologues in rodents and mice. The extracellular domain has four glycosylation spots that are N-linked. The FSH binding activates the G protein bound to the receptor. The receptor is broken by the G protein, which releases the FSH and activates the cAMP system. The signal chain that is formed by the G protein activates protein kinases, which includes the cyclic AMP-dependent protein kinases.
It is also known as the vascular FSHR. FSHR is found in tumor-associated vascular epithelial cells. This transmembrane-related protein was first discovered in human prostate cells. This could be the reason this receptor is so mutagenic. While the function of FSHR is not yet understood, it has been shown to have beneficial effects on the development and treatment of cancer.
In a recent study, researchers found that the FSHR marker found in caveolae of HUVECs interacted with caveolin-1 and GaS. These interactions also slowed the effects of FSH on caveolae. These results suggested that caveolae could be a signaling system that functions. Additionally, FSH increased monocyte adhesion to HUVECs. And a VCAM-1-neutralizing antibody blocked the effects of FSH on monocyte adhesion in humans.
Boster Bio FSHR marker binds to follicle-stimulating hormone (FSH) in caveolae as well as HUVECs. Caveolae with high levels caveolin-1 were found to contain the FSHR marker. Caveolin-1 is a cellular protein that interacts with follicle-stimulating hormone.
The FSHR marker, also known as FSHR is activated by PI3K which is a vital component of the PI3K signaling pathway. This pathway triggers Akt/mTOR as well as PKA, which are the two main elements in the FSHR signaling. Furthermore, Boster Bio FSHR marker is able to interact with follicle-stimulating hormone via GaS/AC/cAMP/PKA cascade.
The Boster Bio FSHR marker has been examined for its FSH binding activity in the laboratory. These experiments were performed using mice in the laboratory. The mice were given FSH for 48 hours, and then left without treatment for 24. After that the mice were fixed in 44% paraformaldehyde over 48 hours and then untreated for 24 hours. Then they were embedded in paraffin wax. The paraffin-embedded sections of tissue were treated using 10 mM citric acid buffer. Then the sections were deparaffinized and blocked in BSA-PBS 5.
FSHR is a key regulator for steroid synthesis. It regulates a variety of nuclear events in granulosa cells that include the process of steroidogenesis. It works in conjunction with the traditional signaling pathway of cAMP/PKA that regulates the transcription factors' activity. This pathway also regulates expression of a rate-limiting enzyme called aromatase. FSHR also activates ERK and PI3K/Akt signaling pathways to regulate specific genes in granulosa cell.
The FSHR gene encodes a transmembrane receptor that has G protein-coupled subunit. It is part of the G-protein coupled receptors family 1. The FSHR protein plays a crucial part in gonad growth. Variations in the gene can cause Ovarian dysgenesis type 1. There are multiple transcript variants for the gene encoding FSHR and includes a subunit that is not functional. The FSHR is the primary contributor to the synthesis of steroid hormones in female foollicles.
FSHR is also regulated by PTMs. They function as molecular switches that regulate gene expression. Anti-inhibin subunit antibodies have been proven to boost FSHR the phosphorylation. Other studies have also proven that FSHR is a target for steroid synthesis in boster Bio. This drug is designed to increase the production of hormones for the ovaries.
As part of the study, a monoclonal antigen against FSHR increased cell proliferation, and slowed down the effects of activin and b-estradiol. This antibody also enhanced transcription factors CEBP and c-FOS. Lh and inhibin are crucial for folliculogenesis. They also regulate the maturation of the granulosa cell.
G protein-coupled receptor FSHR has extracellular and intracellular domains. It regulates steroidogenesis as well as the growth of follicles. The receptor is involved in the conversion of androgens to estrogens and controls the production of Ovarian Granules. Mutations in FSHR are linked to primary amenorrhoea.
To identify FSHR in mouse sperm, we used real-time reverse-transcription polymerase chain reaction (qRT-PCR). The mRNA levels for FSHR, LHR were determined in the testis and gonad using techniques for cloning. We were able identify the mRNAs of FSHR, LHR, and their respective gene products.
FSHR is associated to increased proliferation of granulosa cell and regulates steroidogenesis through its effects on the signaling pathways PKA/cAMP. It also activates aromatase a rate-limiting enzyme that controls the process of steroidogenesis. In addition to controlling FSHR expression, FSHR is also associated with the activation of ERK and the PI3K/Akt signaling pathways found in cells that are granulosa.
Moreover, FSHR has a strong affinity to the GPCRs whose crosstalk with ubiquitin promotes its degrading. Additionally, FSHR is phosphorylated by the proteasome. The proteasome inhibitor MG132 raised FSHR levels in the ewe's uterus. BDNF and FSH also reduced FSHR levels of protein.
To monitor OHSS, an FSHR marker is a good choice. The amount of haptoglobin, fibrinogen, and lipoprotein lipase in serum is a signpost for the blood's ability to coagulate. These proteins play a crucial role in the health of the body as well as their function, including the immune system's natural defense system, blood coagulation and the activation of platelets as well as arterial restructuring.
There are many genetic variants that are part of the FSHR gene. They are characterized by varying specificity and sensitivities. One such variant is rs1394205, which is located in the 5' untranslated core promoter region of the gene. This variant is widespread, but not well-studied. It causes a decrease in transcriptional activity due to the fact that it swaps the G-type allele for an A-type allele. It is also associated with a poor response to ovarian stimulation.
The FSHR Marker can be used to predict OHSS and COS outcomes. This marker has high sensitivity as well as specificity, and is comparable to BMI. Clinical interpretation of the positive FSHR Marker for OHSS is based on clinical symptoms and the results of other studies. These findings are a fantastic initial step in treating OHSS.
In addition to a serum FSHR Marker the diagnosis by clinical examination of OHSS could be a bit difficult. It is challenging to pick one preventative measure since the benefits and risks of COS differ greatly from person to person. Biomarkers can also be helpful for identifying patients who require individualized COS to treat the condition. These markers can help doctors tailor a COS protocol for each patient, and also identify risk factors that could increase the risk of developing OHS.
The FSHR, also known as the name follicle-stimulatinghormone is a transmembrane receptor that belongs to the G protein-coupled receptors family 1. It is a key gene in gonad and ovarian dysgenesis type 1 mutations. There are numerous transcript variants that result from alternative splicing. Based on positive and negative samples Boster Bio tests can identify mutations in the FSHR.
FSHR isoforms were identified in human, mouse sheep, rat, and human samples. Four transcripts are encoded by the FSHR gene. Each carries a unique mRNA sequence. Two splice variants of FSHR protein are available that include alternative splicing and exon deletion. The FSHR mRNA is identical to the full-length protein sequence. However the alternative splice variants do not have the same cell functions. However, these variants could be possibly important for research.
A tiny deletion of the FSHR gene could result in the absence of immunoactive FSH in human blood. The mutation would also cause the loss of all seven TMD1 -s and three ICL1-3 domains. The mutant protein will have an incomplete ECD, with only two extracellular domains. It would also have a mutated Tyr 335 and a truncated hinge.
In human cancers, FSHR is expressed in extragonadal tissues. If it is expressed in TVECs, this could result in a novel approach to cancer treatment. To determine the role of FSHR further research is required. The Boster Bio technique can also be used to detect mutations within the FSHR. This technique is extremely sensitive and has a high degree of sensitivity.
PMID: 1709010 by Minegish T., et al. Cloning and sequencing of human FSH receptor cDNA.
PMID: 1322283 by Tilly J.L., et al. Expression of recombinant human follicle-stimulating hormone receptor: species-specific ligand binding, signal transduction, and identification of multiple ovarian messenger ribonucleic acid transcripts.
*More publications can be found for each product on its corresponding product page