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- Table of Contents
GSTA2 / Glutathione S-transferase A2 · Western blot design guide
Design a Western Blot for GSTA2
Source-linked GSTA2 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-GSTA2 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
GSTA2 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~25.7 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Duodenum (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Acetylated | |
| Caveat | Band size unreported | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked GSTA2 Western Blot Protocol Options
The A04171 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | extracts of various cells, (catalog A04171) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A04171; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected GSTA2 Western Blot Band Size?
GSTA2's predicted monomer is 25.7 kDa; dimerization could affect migration under native conditions, but no empirical Western blot band size is supplied.
| Single band near 25.7 kDa | Consistent with the predicted GSTA2 monomer; confirm identity with antibody controls |
| Band near twice the monomer size under native conditions | Could reflect a retained GSTA2-containing dimer |
| Monomer band plus a higher band under native conditions | Could reflect both free GSTA2 and retained dimers |
| Little or no band in a membrane-only fraction | Consistent with GSTA2's cytoplasmic location |
💡Expected GSTA2 appearanceThe predicted GSTA2 monomer is 25.7 kDa; no empirical Western blot band size is supplied, so confirm any candidate band with antibody specificity controls.
| Predicted GSTA2 monomer mass | Provides a 25.7 kDa reference for the subunit |
| GSTA2 homodimer | Could appear near twice the monomer size if association survives sample preparation |
| GSTA1–GSTA2 heterodimer | Could produce a higher apparent size if the complex remains associated; its mass is not supplied |
| Dissociation of GSTA2-containing dimers | Returns associated subunits toward their individual apparent sizes under denaturing conditions |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Cytoplasmic GSTA2 may be lost during fractionation | Check the soluble cytoplasmic fraction and a loading control |
| Band higher than expected | A GSTA2-containing dimer may remain associated | Compare native and fully denatured samples; confirm band identity |
| Band lower than expected | Possible sample degradation; no cleavage feature is listed | Prepare fresh lysate with protease inhibitors and verify antibody specificity |
| Multiple bands | Possible retained dimers alongside monomers or antibody cross-reactivity | Compare denaturation conditions and use an independent antibody or depletion control |
| Weak or no signal | Insufficient cytoplasmic protein or poor transfer | Check protein loading, the soluble fraction, and transfer efficiency |
Experiment evidence
Western blot images showing GSTA2 band
A04171 · GSTA2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for GSTA2 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for GSTA2 in Western blot, you can use duodenum lysate, which HPA rates high for expression.
Positive control: Duodenum (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: Cytoplasmic GSTA2 should be accessible in tissue lysate, and HPA lists several tissues where it is not detected.
HPA tissue expression evidence for GSTA2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Duodenum | endocrine cells | High | Protein (IHC) | HPA → |
| Liver | hepatocytes | High | Protein (IHC) | HPA → |
| Testis | Leydig cells | High | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Medium | Protein (IHC) | HPA → |
| Fallopian tube | glandular cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Appendix | endocrine cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
| Caudate | glial cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced GSTA2 Western Blot Tips
Deeper troubleshooting and optimisation questions for GSTA2, answered from its protein features.
Where should the GSTA2 band appear relative to its predicted mass?
Band shift · GSTA2 has a predicted mass of 25.7 kDa. No observed Western blot band is supplied, so an apparent mass difference cannot be assessed from these features.
Should multiple GSTA2 isoform bands be expected?
Isoforms · The supplied record lists one isoform and no alternative sequence. It therefore provides no isoform-based explanation for multiple bands.
Could GSTA2 modifications change its apparent band position?
PTM · UniProt lists N-acetylalanine at position 2 and N6-succinyllysine at position 4, using UniProt sequence numbering. These features alone do not establish a visible band shift or explain a difference from 25.7 kDa.
No glycosylation sites are listed for GSTA2. The supplied features do not support attributing a higher band to glycosylation.
No glycosylation sites are listed for GSTA2. The supplied features do not support attributing a higher band to glycosylation.
Does this guide establish induction of GSTA2?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for GSTA2?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A04171 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
What should be checked before quantifying a GSTA2 band?
Quantitation · Identify the band assigned to GSTA2 against its 25.7 kDa predicted mass. Because GSTA2 can associate with GSTA1, confirm which band the antibody detects before assigning a higher band to GSTA2 or including it in quantitation.
How should higher bands be interpreted given GSTA2 dimerization?
Interpretation · GSTA2 can form a homodimer or a heterodimer with GSTA1. Consider that property when investigating a higher band, but the oligomer annotation alone does not establish that a dimer survives Western blot preparation or causes the band.
UniProt places GSTA2 in the cytoplasm. Check a cytoplasmic fraction when assessing its presence; the supplied features do not establish its abundance in any particular sample.
UniProt places GSTA2 in the cytoplasm. Check a cytoplasmic fraction when assessing its presence; the supplied features do not establish its abundance in any particular sample.
Boster reagents
GSTA2 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Glutathione S-transferase A2 GSTA2 Antibody
Cat # A04171
A04171 is an anti-GSTA2 antibody listed for Western blot with reported human, mouse, and rat reactivity. Its WB image is described as using extracts of various cells; the supplied evidence does not identify the cells or blot conditions.
Which to pick: A04171 is the only listed option for GSTA2 and has a WB image. Check that its reported reactivity matches your species; the supplied image description does not specify the tested cells.
Source: BosterBio GSTA2 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P09210.
- Human Protein Atlas. GSTA2 tissue expression.
- PMC4437487 — target-verified WB comparison
- PMC8295304 — target-verified WB comparison
- PMC9917430 — target-verified WB comparison