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- Table of Contents
Facts about Hematopoietically-expressed homeobox protein HHEX.
May play a role in hematopoietic differentiation. Establishes anterior identification at two levels; acts early to boost canonical WNT-signaling by repressing expression of TLE4, and behaves later to inhibit NODAL-signaling by directly targeting NODAL (By similarity).
Human | |
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Gene Name: | HHEX |
Uniprot: | Q03014 |
Entrez: | 3087 |
Belongs to: |
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No superfamily |
hematopoietically expressed homeobox; Hex; HEXHMPH; HHEX; HMPH; Homeobox protein HEX; Homeobox protein PRH; HOX11L-PEN; PRH; PRHhematopoietically-expressed homeobox protein HHEX; PRHX; PRHXhomeobox, hematopoietically expressed; proline-rich homeodomain-containing transcription factor
Mass (kDA):
30.022 kDA
Human | |
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Location: | 10q23.33 |
Sequence: | 10; NC_000010.11 (92689955..92695647) |
Liver and promyelocytic leukemia cell line HL- 60.
Nucleus.
This article covers Boster Bio: What It Is, How It Works, And Why It Is Great For Scientific Research. Also covered are secondary antibodies, isotype controls, and detection systems. If you're an aspiring scientist, this article is for you! Learn more about these products and how they can help your work. Then, share your results with others, and you could even get product credits!
There are two types of antibodies: primary and secondary. Primary antibodies bind to antigens or proteins directly, while secondary antibodies target another antibody. While primary antibodies can bind directly to the antigen, they are often useless on their own. This article explores both types and provides useful information to help you choose the right one for your needs. You'll be glad you did when you're done reading! Continue reading to learn more about secondary antibodies and their uses.
The Secondary Antibodies market is composed of different entities. This study also covers opportunities, trends, and regulatory compliance. Companies are listed in the global Secondary Antibody market study, with company profiles, business overview, and company snapshots. Secondary research sources include company websites, encyclopedias, and Meta-analyses. The report includes key market drivers and challenges, and is broken down by region and country. If you're interested in the Secondary Antibodies market, get ready to become the next biotech mogul.
Affinity-isolated secondary antibodies have the lowest amount of non-specific binding. They are ideal for research where rare or low-abundance antigens are involved. However, when it comes to antigens, the latter may require high affinity antibodies. Antibodies with high affinity may be difficult to elute and must be carefully chosen. You should also look for information about the animal that was immunized with the antigen.
As you can see, secondary antibodies are useful in many applications. They can recognize primary antibodies and bind with them. They can be conjugated with a reporter molecule, such as a fluorescent dye, or they can even be enzymes that convert a substrate to a dye. Moreover, secondary antibodies are more versatile than primary antibodies. Some of them are available in commercialized forms and can be pre-conjugated with several common labels. Some are also biotinylated, which enhances the signal.
When purchasing secondary antibodies, it's important to know about their storage and reconstitution. Typically, liquid secondary antibodies are ready for use straight out of the vial, while lyophilized ones must be reconstituted in a buffer that is appropriate for the application. This is because lyophilized secondary antibodies are susceptible to degradation by freezing and thawing, which reduces their staining capacity. There are advantages and disadvantages to each format, and the choice is yours.
Isotype controls help you determine the sensitivity of your primary antibodies. There are three types of non-specific background staining: a binding to FC receptors on non-target cells, interactions with other proteins or sugars, and binding to lipids. To detect the presence of non-specific staining, run isotype controls against each sample. Non-specific staining should prompt further refinement of the assay.
Isotype controls help determine background signal in applications such as immunohistochemistry and flow cytometry. They mimic the antibodies that the primary antibody detects. Isotype controls are important for ensuring the specificity of the antibodies used in the study. They are available in various species and light chains. While there is no universal isotype control for every antibody, isotype controls are a useful tool for assessing the sensitivity of different primary antibodies.
An isotype control is an antibody that is produced to match the properties of the primary antibody. These antibodies match host species, subclass, and conjugation, but lack specificity. They are used to ensure that the primary antibody does not bind to a particular epitope. An isotype control can also be used as a coating agent for antibodies. When used in immuno-oncology research, they can help identify the potential for positive and negative-reactions.
Information about isotype controls in Boster bio antibodies should be available when ordering. In addition to the primary antibody, isotype controls may be used in blocking assays, immunohistochemistry, or immunofluorescent applications. Information about isotype controls can help you make the right choice for your research. Whether it's an IHC assay or a blocking test, Boster Bio antibodies can help you find the right antigen for your work.
Intrusion detection systems (IDS) are software applications that monitor network traffic for suspicious activity and issue alerts if anything goes wrong. The IDS typically monitors traffic and examines hosts to detect malicious activity. It collects information about incoming and outgoing network traffic and then uses alarm filtering techniques to distinguish between legitimate traffic and malicious activities. These systems are installed by an organization and must be properly configured to ensure that they monitor all network traffic.
A host intrusion detection system is a network intrusion prevention system that is installed on an independent host. It examines incoming and outgoing network traffic and notifies the administrator when it detects suspicious activity. It also compares system files against a previous snapshot to detect suspicious activity. It can also detect Trojan horses and other software-related threats. It operates in encrypted network environments. Information about detection systems is useful when you have a mission-critical machine and don't want your layout to change.
PMID: 1360645 by Crompton M.R., et al. Identification of a novel vertebrate homeobox gene expressed in haematopoietic cells.
PMID: 8103988 by Hromas R.A., et al. PCR cloning of an orphan homeobox gene (PRH) preferentially expressed in myeloid and liver cells.