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- Table of Contents
HINT1 Antibodies
AND HINT1 ELISA kits, HINT1 Proteins
Many biological assays use antibodies to detect HINT1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with HINT1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop HINT1 antibodies...
Designing a Western blot for HINT1? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the HINT1 Western Blot GuideDesigning an IHC experiment for HINT1? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the HINT1 IHC GuideHINT1 Infographic by Boster Bio
All HINT1 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # PB9632
100 μg/vial
Cat # PB9632
100 μg/vial
Human, Mouse, Rat
ELISA
$480
Cat # EKC1732
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1732
1 kit, containing one 96-well plate and all necessary reagents
Human, Mouse, Rat
IF, IHC, ICC, WB
$370
Cat # M02557-1
100 μl/vial
Cat # M02557-1
100 μl/vial
HINT1 Overview
Facts about Histidine triad nucleotide-binding protein 1.
Histidine triad nucleotide-binding protein 1 (HINT1)
Hydrolyzes purine nucleotide phosphoramidates with one phosphate group, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) made by lysine tRNA ligase, as well as Met-AMP, His- AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester.
Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O- phosphorothioate into the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and from the complex formed with MITF and CTNNB1.
Gene Information
| Human | |
|---|---|
| Gene Name: | HINT1 |
| Uniprot: | P49773 |
| Entrez: | 3094 |
Family
| Belongs to: |
|---|
| HINT family |
Alternative Names
Adenosine 5'-monophosphoramidase; EC 3.-; HINT; histidine triad nucleotide binding protein 1; histidine triad nucleotide-binding protein 1; histidine triad nucleotide-binding protein; PKCI1; PKCI-1FLJ30414; PRKCNH1FLJ32340; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1
Molecular Weight
Mass (kDA):
13.802 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 5q23.3 |
| Sequence: | 5; NC_000005.10 (131159027..131165348, complement) |
Tissue Specificity
Widely expressed.
Subcellular Localizations
Cytoplasm. Nucleus. Interaction with CDK7 leads to a more nuclear localization.
Diseases
- Neoplasms
- Malignant Neoplasms
- Schizophrenia
- Carcinoma
- Liver Carcinoma
- Depressive Disorder
- Liver Neoplasms
- Carcinogenesis
- Cell Transformation, Neoplastic
- Malignant Tumor Of Colon
- Mental Disorders
- Major Depressive Disorder
Pathways
- Cell Proliferation
- Cell Growth
- Cell Cycle
- Dna Repair
- Localization
- Methylation
- Cell Activation
- Cell Migration
- Locomotion
- Pathogenesis
- Rna Interference
- Transport
- Muscle Cell Migration
PTMs
- Phosphorylation
- Methylation
- Amidation
- Cleavage
- Demethylation
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/HINT1
bosterbio.com/bosterbio-gene-info-cards/HINT1
References
PMID: 8812426 by Brzoska P.M., et al. Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1).
PMID: 7644499 by Brzoska P.M., et al. The product of the ataxia-telangiectasia group D complementing gene, ATDC, interacts with a protein kinase C substrate and inhibitor.