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- Table of Contents
HINT1 antibody (M02557) performs well in Flow Cytometry, IF, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Overlay histogram showing Jurkat cells stained with M02557 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M02557, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Anti-HINT1 Antibody
HINT1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | Flow Cytometry, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
IF: 1:25
WB: 1:4000
IHC-P: 1:25
FC: 1:25
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-HINT1 Antibody
View all HINT1 Antibodies
View all HINT1 Antibodies
Uniprot
Gene ID
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1500CT836.13.93
Isotype
IgG1,k
Immunogen
This HINT1 antibody is generated from a mouse immunized with a recombinant protein of human HINT1.
Calculated Molecular Weight
13.8 kDa
Background of HINT1
Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His- AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O- phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin- protein ligase complex.
Cite This Product
Anti-HINT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02557)
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Product Promise
We promise Anti-HINT1 Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M02557
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026