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- Table of Contents
HOXA13 / Homeobox protein Hox-A13 · Western blot design guide
Design a Western Blot for HOXA13
Source-linked HOXA13 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-HOXA13 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
HOXA13 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~39.7 kDa | |
| Gel | 12–15% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked HOXA13 Western Blot Protocol Options
The M03305 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | 293T cell lysate (catalog M03305) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | M03305; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected HOXA13 Western Blot Band Size?
HOXA13 has a predicted monomer mass of 39.7 kDa; homodimerization could affect migration, but no empirical band or gel-stable dimer is demonstrated.
| Band near 39.7 kDa | Consistent with the predicted HOXA13 monomer; identity needs confirmation. |
| Band near 79 kDa | Could reflect a retained HOXA13 homodimer; gel stability is unproven. |
| Band enriched in the nuclear fraction | Consistent with HOXA13 nuclear localization. |
| Little or no band in the cytosolic fraction | Consistent with HOXA13 nuclear localization; check the nuclear fraction. |
💡Expected HOXA13 appearanceUniProt predicts a 39.7 kDa HOXA13 monomer, but no empirical band size is supplied; confirm any candidate band with appropriate antibody and fractionation controls.
| UniProt predicted molecular weight | Sets a 39.7 kDa reference for the monomer. |
| 39,727 Da predicted mass | Expresses the same monomer reference as approximately 39.7 kDa. |
| DNA-binding homodimer | Could give an approximately 79 kDa species if the dimer survives sample preparation. |
| Homodimer stability during electrophoresis | Determines whether a larger species appears; no disulfide bond is listed. |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | HOXA13 may be poorly recovered during nuclear extraction. | Check the nuclear fraction and a nuclear recovery control. |
| Band higher than expected | A retained homodimer is possible, but its gel stability is unproven. | Compare sample preparation conditions and confirm identity with another antibody. |
| Band lower than expected | The supplied features do not establish a smaller HOXA13 product. | Confirm identity with another antibody and check sample integrity. |
| Multiple bands | Monomer and retained homodimer are possible; distinct isoforms are not listed. | Compare preparation conditions and verify each band independently. |
| Weak or no signal | The nuclear protein may be underrepresented in the tested fraction. | Check nuclear recovery and antibody performance with a suitable control. |
Experiment evidence
Western blot images showing HOXA13 band
M03305 · HOXA13
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for HOXA13 Western blot
🧪For positive controls for HOXA13 in Western blot, you can use a verified HOXA13-positive sample, although the supplied HPA evidence identifies none.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA provides no tissue data, so a positive sample needs independent validation and a knockdown or KO provides a practical negative control.
HPA tissue expression evidence for HOXA13
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced HOXA13 Western Blot Tips
Deeper troubleshooting and optimisation questions for HOXA13, answered from its protein features.
How should HOXA13 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could a second band be a listed HOXA13 isoform?
Isoforms · Only one isoform is listed, with no alternative sequence. The supplied features therefore do not support assigning a second band to a known HOXA13 isoform.
Do listed modifications explain a shifted HOXA13 band?
PTM · No modified residues or glycosylation sites are listed. The supplied features cannot attribute a shift to a specific modification, and they do not rule out modifications that are unlisted.
Does this guide establish induction of HOXA13?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for HOXA13?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M03305 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should HOXA13 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
Should HOXA13 migrate exactly at its predicted mass?
Interpretation · The predicted mass is 39.7 kDa, but no observed Western blot band is supplied. Use 39.7 kDa as a starting reference, not a confirmed migration position; these features do not establish a band shift.
HOXA13 is listed as nuclear. Check the nuclear fraction when assessing whether a candidate band matches its reported location, and compare like fractions across samples.
HOXA13 binds DNA as a homodimer. That feature alone does not show that a dimer survives Western blot preparation or produces a visible band; do not identify a higher band as a dimer on this basis alone.
Compare candidate bands with the 39.7 kDa predicted mass and HOXA13's nuclear location. No observed band, alternative isoform, or modified residue is supplied, so the features cannot identify an unexpected band.
Measure the same candidate band in comparable fractions and use a consistent normalization method. HOXA13 is nuclear, and its observed band position is unavailable, so establish the band assignment before comparing signal levels.
HOXA13 is listed as nuclear. Check the nuclear fraction when assessing whether a candidate band matches its reported location, and compare like fractions across samples.
HOXA13 binds DNA as a homodimer. That feature alone does not show that a dimer survives Western blot preparation or produces a visible band; do not identify a higher band as a dimer on this basis alone.
Compare candidate bands with the 39.7 kDa predicted mass and HOXA13's nuclear location. No observed band, alternative isoform, or modified residue is supplied, so the features cannot identify an unexpected band.
Measure the same candidate band in comparable fractions and use a consistent normalization method. HOXA13 is nuclear, and its observed band position is unavailable, so establish the band assignment before comparing signal levels.
Boster reagents
HOXA13 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-HOXA13 Rabbit Monoclonal Antibody
Cat # M03305
The catalog reports one anti-HOXA13 Western blot antibody, M03305, a rabbit monoclonal reported to react with human, mouse, and rat. Its WB image shows HOXA13 expression in 293T cell lysate; the supplied evidence does not show other tested specimens.
Which to pick: M03305 is the only listed option and has a WB image using 293T cell lysate. Check its reported human, mouse, and rat reactivity against your planned sample; the supplied image documents the 293T context only.
Source: BosterBio HOXA13 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P31271.
- Human Protein Atlas. HOXA13 tissue expression.
- PMC12395676 — target-verified WB comparison
- PMC6404682 — target-verified WB comparison
- PMC10374758 — target-verified WB comparison