IFT88 / Intraflagellar transport protein 88 homolog · Western blot design guide

Design a Western Blot for IFT88

Real validated IFT88 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-IFT88 WB antibodies. Everything you need to plan the experiment before you commit precious samples.

Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for IFT88: expected band ~93.2 kDa, hero antibody A06814-1, catalog values and labelled standard workflow; separate PMC comparisons on the guide — Printable **IFT88** Western blot protocol sheet — expected band ~93.2 kDa, antibody A06814-1, controls and PMC citations. Open the full IFT88 WB guide →

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IFT88 Western Blot Experimental Design Guide

Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	~93.2 kDa
	Observed band	~94 kDa
	Gel	5–20% (catalog A06814-1)
	Negative control ⓘ	Suggested KO / knockdown lysate

Important caveats

Reasons your observed band may differ from the expected size.

	PTM	—
	Caveat	—
	Gene-set association	MSigDB Hallmark membership
	Isoform	3 isoform(s)

Section 1

Real Curated IFT88 Western Blot Protocols

The A06814-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.

Recommended Western blot protocol parameters
Sample / lysate	rat testis, mouse testis (catalog A06814-1)
Gel %	5–20% (catalog A06814-1)
Load	50ug; reducing conditions (catalog A06814-1)
Transfer	a Nitrocellulose membrane at 150mA for 50-90 minutes (catalog A06814-1)
Membrane	Nitrocellulose membrane (catalog A06814-1)
Blocking	5% Non-fat Milk/ TBS for 1.5 hour at RT (catalog A06814-1)
Primary antibody	A06814-1 · 0.5 μg/mL (catalog A06814-1)
Primary incubation	overnight at 4°C (catalog A06814-1)
Secondary antibody	goat anti-rabbit IgG-HRP, 1:5000 (catalog A06814-1)
Secondary incubation	1.5 hour at RT (catalog A06814-1)
Wash	TBS-0.1%Tween 3 times with 5 minutes each (catalog A06814-1)
Detection	ECL (catalog A06814-1)

Section 2

What Is the Expected IFT88 Western Blot Band Size?

IFT88 is predicted at 93.2 kDa and observed near 94 kDa; the cause of the small difference is not established.

What am I looking at on my blot?
Single band near 94 kDa	Matches the observed IFT88 band and its 93.2 kDa predicted mass.
Additional band at another size	Could represent an IFT88 splice isoform; band identity needs confirmation.
Weak band near 94 kDa	May reflect limited IFT88 in the sampled lysate.
No band near 94 kDa	IFT88 may be scarce in the sampled lysate.

💡Expected IFT88 appearanceIFT88 has a predicted mass of 93.2 kDa and an observed band near 94 kDa; confirm band identity with an appropriate specificity control.

How each factor affects band size
Predicted IFT88 mass	93.2 kDa predicts a band close to the observed 94 kDa.
Splice isoform 1	May differ in size from other isoforms; its individual mass is unreported.
Splice isoform 2	May differ in size from other isoforms; its individual mass is unreported.
Splice isoform 3	May differ in size from other isoforms; its individual mass is unreported.

Why is my band missing or off?
Situation	Likely cause	Next action
No band in lysate	IFT88 may be scarce in the sampled material.	Check sample quality and use a known IFT88-positive lysate.
Band higher than expected	An unassigned isoform or unrelated antibody signal is possible.	Compare with an IFT88-depleted control.
Band lower than expected	An unassigned isoform or protein breakdown is possible.	Compare with an IFT88-depleted control and check sample handling.
Multiple bands	Three IFT88 splice isoforms are listed, but their migration is unreported.	Test which bands disappear after IFT88 depletion.
Weak or no signal	IFT88 may be scarce in the tested sample.	Check loading and compare with a known IFT88-positive sample.

Source: UniProt Q13099 Human Protein Atlas product datasheet (A06814-1)

Experiment evidence

Western blot images showing IFT88 band

A06814-1 · IFT88

M06814 · IFT88

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Real WB data behind the band-size expectations in this section.

Sample controls for IFT88 Western blot

🧪For positive controls for IFT88 in Western blot, you can use a sample with confirmed IFT88 expression; the supplied HPA data identify no positive tissue or cell line.

Positive control: No high/medium HPA tissue identified

Negative control: Suggested KO / knockdown lysate

Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.

⚠️Feasibility: The supplied HPA data identify no tissue controls, so positive expression must be confirmed and a knockdown or KO can provide a negative control.

HPA tissue expression evidence for IFT88

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.

Higher expression tissues · candidate positive controls from IHC

Tissue	Cell type	Level	Evidence	Source
No high/medium HPA tissues identified in the supplied evidence.

Lower expression tissues · IHC evidence, not confirmed WB-negative controls

Tissue	Cell type	Level	Evidence	Source
No lower-expression tissue rows available in the supplied evidence.

Section 3

Advanced IFT88 Western Blot Tips

Deeper troubleshooting and optimisation questions for IFT88, answered from its protein features.

How should IFT88 band migration be interpreted?

Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.

Which sequence changes distinguish IFT88 isoforms?

Isoforms · UniProt lists three isoforms. In isoforms 1 and 3, the initial M is replaced by MKFTNTKVQM. Isoform 3 also lacks residues 52–70, using the supplied UniProt coordinates.

The listed sequence changes could affect migration, but the features do not establish whether the isoforms resolve as separate bands. Compare any additional bands with the expected IFT88 region and assess antibody specificity before assigning an isoform.

Do annotated modifications prove a band shift?

PTM · The linked UniProt record describes protein features. A modification annotation alone does not demonstrate a visible shift; retain any condition or experimental qualifier attached to it.

Does this guide establish induction of IFT88?

Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.

What transfer method to use for IFT88 Western blot?

Transfer · IFT88 is predicted at 93.2 kDa. Choose transfer conditions that efficiently recover proteins around 94 kDa, and check transfer in that region with a molecular weight marker. The supplied features do not specify a preferred transfer method.

How should blocking be optimized?

Blocking · Standard workflow guidance: follow the A06814-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.

How should IFT88 Western blots be quantified?

Quantitation · Quantify the band near 94 kDa consistently across matched samples. IFT88 is reported at cilia, basal bodies and centrosomes, so use comparable sample fractions when interpreting changes in band intensity.

Why is the IFT88 band near 94 kDa?

Interpretation · The observed band is approximately 94 kDa, close to the predicted 93.2 kDa. The supplied features do not establish a cause for the small difference between apparent and predicted mass.

IFT88 is a component of IFT complex B and interacts with IFT20, IFT22 and IFT25. Complex membership alone does not establish that a high band on a Western blot is an IFT88-containing complex; verify its identity before assigning it.

Boster reagents

IFT88 Western Blot Antibodies

Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.

Real WB data

Western blot analysis of IFT88 using anti-IFT88 antibody (A06814-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT88 antigen affinity purified polyclonal antibody (Catalog # A06814-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFT88 at approximately 94KD. The expected band size for IFT88 is at 94KD.

Anti-IFT88 Antibody Picoband®

Cat # A06814-1

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Real WB data Western blot analysis of IFT88 expression in HepG2 cell lysate.

Anti-IFT88 Rabbit Monoclonal Antibody

Cat # M06814

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Two the supplier anti-IFT88 antibodies list Human, Mouse, and Rat reactivity and have WB images. A06814-1 shows an approximately 94 kDa band in rat and mouse testis lysates; M06814 shows IFT88 expression in HepG2 lysate. Evidence is limited to these reported examples.

Which to pick: For rat or mouse testis lysates, A06814-1 has a detailed WB example with an approximately 94 kDa band. For HepG2 lysate, M06814 has a WB image. Both list Human, Mouse, and Rat reactivity; choose by the closer reported sample context.

Source: BosterBio IFT88 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.

References

UniProt Consortium. UniProt entry Q13099.
Human Protein Atlas. IFT88 tissue expression.
PMC5865803 — target-verified WB comparison
PMC8771550 — target-verified WB comparison
PMC7066128 — target-verified WB comparison