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- Table of Contents
IL10RA / Interleukin-10 receptor subunit alpha · Western blot design guide
Design a Western Blot for IL10RA
Source-linked IL10RA Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-IL10RA WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
IL10RA Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~63 kDa | |
| Gel | 12–15% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Glycosylated + Phosphorylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked IL10RA Western Blot Protocol Options
The A03597 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Target-positive lysate and matched negative control (standard starting point) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A03597; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected IL10RA Western Blot Band Size?
IL10RA has a predicted 63 kDa precursor; glycosylation and signal-peptide cleavage may affect migration, but no empirical band size is available.
| Band near 63 kDa | Consistent with the predicted precursor mass; identity requires controls |
| Band above 63 kDa | N-linked glycans could increase apparent mass |
| Band slightly below 63 kDa | Signal-peptide cleavage could reduce mass |
| Broad smear | Variable N-linked glycosylation is possible |
| Multiple nearby bands | Different glycosylation states are possible; identity requires controls |
💡Expected IL10RA appearanceUniProt predicts a 63 kDa precursor; signal-peptide cleavage and N-linked glycosylation may alter migration, but no empirical band size is supplied, so confirm any candidate band with identity controls.
| Predicted precursor mass | Provides a 63 kDa sequence-based reference |
| N-linked glycosylation at Asn50, Asn74 and Asn110 | May increase apparent mass if these sites carry glycans |
| N-linked glycosylation at Asn154, Asn177 and Asn189 | May increase apparent mass if these sites carry glycans |
| Signal peptide at residues 1–21 | Cleavage makes the mature protein smaller than the precursor |
| Situation | Likely cause | Next action |
|---|---|---|
| Band higher than expected | N-linked glycosylation may alter migration | Compare untreated and deglycosylated samples with an identity control |
| Band lower than expected | Signal-peptide cleavage may lower mass | Check whether the antibody recognizes the mature receptor and confirm identity |
| Broad smear instead of sharp band | Variable glycosylation at the six N-linked sites is possible | Compare with a deglycosylated aliquot |
| Multiple bands | Different glycosylation states or unrelated antibody binding are possible | Compare deglycosylated samples and verify bands with a second antibody |
| Weak or no signal | The membrane receptor may be poorly recovered in the lysate | Check membrane extraction and include a positive control |
Experiment evidence
Western blot images showing IL10RA band
A03597 · IL10RA
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for IL10RA Western blot
🧪For positive controls for IL10RA in Western blot, you can use an HPA-confirmed positive sample once one is identified.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA provides no tissue data here, so a positive sample cannot be selected and a negative control needs knockdown or knockout validation.
HPA tissue expression evidence for IL10RA
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced IL10RA Western Blot Tips
Deeper troubleshooting and optimisation questions for IL10RA, answered from its protein features.
What should be checked when unexpected IL10RA bands appear?
Band shift · Check whether the bands track with the annotated signal peptide at residues 1–21, six N-linked sites, and the preparation's reducing conditions. These features suggest points to investigate; they do not identify an unexpected band or prove its cause.
Should multiple IL10RA bands be assigned to isoforms?
Isoforms · The supplied record lists one isoform and no alternative sequence. Do not assign additional bands to specific IL10RA isoforms from these features alone.
Which IL10RA glycosylation sites matter when interpreting bands?
PTM · UniProt annotates N-linked asparagines at positions 50, 74, 110, 154, 177, and 189, using the supplied 578-residue sequence numbering. Different numbering conventions may appear elsewhere. Site annotations alone do not establish occupancy or explain a particular band.
Does this guide establish induction of IL10RA?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for IL10RA Western blot?
Transfer · IL10RA is a 63 kDa predicted, single-pass type I membrane protein. Choose and verify transfer conditions suitable for that size, checking transfer efficiency on the membrane. The supplied features do not establish a uniquely best transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A03597 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should IL10RA bands be quantified?
Quantitation · Define the band or band region consistently across samples, check that detection is within a usable range, and normalize consistently. The supplied features provide a predicted mass but no observed band position, so they cannot identify which band to quantify.
Why might IL10RA migrate differently from its predicted mass?
Interpretation · IL10RA has a predicted mass of 63 kDa, a signal peptide at residues 1–21, and six annotated N-linked glycosylation sites. These features are relevant when assessing migration, but the supplied data give no observed band size and do not establish a visible shift.
UniProt lists two disulfide bonds. When comparing bands from reducing and nonreducing samples, keep the preparation conditions consistent and consider those bonds in interpreting differences. Their presence alone does not predict a specific mobility change.
UniProt lists two disulfide bonds. When comparing bands from reducing and nonreducing samples, keep the preparation conditions consistent and consider those bonds in interpreting differences. Their presence alone does not predict a specific mobility change.
Boster reagents
IL10RA Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-IL-10 alpha IL10RA Antibody
Cat # A03597
A03597 is a polyclonal anti-IL10RA antibody listed as reactive with Human and Mouse samples. The supplied record includes a WB image and a caption describing SDS-PAGE analysis of cells. No publication evidence is supplied.
Which to pick: A03597 is the only listed antibody. It has a WB image and reports Human and Mouse reactivity; check the full validation details for a sample and conditions relevant to your experiment.
Source: BosterBio IL10RA gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q13651.
- Human Protein Atlas. IL10RA tissue expression.
- PMC7607837 — target-verified WB comparison
- PMC8900811 — target-verified WB comparison
- PMC13267665 — target-verified WB comparison