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- Table of Contents
IL11 / Interleukin-11 · Western blot design guide
Design a Western Blot for IL11
Real validated IL11 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-IL11 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
IL11 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~21.4 kDa | |
| Gel | 15% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Cleaved | |
| Caveat | Secreted protein | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 2 isoform(s) |
Section 1
Real Curated IL11 Western Blot Protocols
The A01160 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Target-positive lysate and matched negative control (standard starting point) |
| Gel % | 15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.2 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A01160; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected IL11 Western Blot Band Size?
IL11 has a predicted 21.4 kDa precursor; secretion, signal-peptide cleavage, and isoforms could affect the pattern, but distinct migration has not been demonstrated here.
| Band near 21.4 kDa | consistent with the predicted IL11 precursor, pending identity controls |
| Band below 21.4 kDa | may reflect removal of the 1–21 signal peptide |
| Little or no band in whole-cell lysate | consistent with IL11 secretion |
| Bands at different sizes | could reflect isoforms 1 and 2, though distinct migration is unproven |
💡Expected IL11 appearanceThe predicted IL11 precursor is 21.4 kDa; signal-peptide cleavage may yield a smaller mature band, but no empirical band size is supplied, so confirm identity with appropriate controls.
| Predicted precursor mass | places full-length IL11 near 21.4 kDa |
| Signal peptide at residues 1–21 | cleavage may make mature IL11 smaller than the precursor |
| Isoform 1 | its size relative to isoform 2 is unspecified |
| Isoform 2 | its size relative to isoform 1 is unspecified |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | IL11 is secreted | check conditioned medium alongside lysate |
| Band higher than expected | an uncleaved precursor may run above mature IL11 | compare lysate and conditioned medium and verify band identity |
| Band lower than expected | signal-peptide cleavage may lower the mass | compare with the precursor prediction and verify band identity |
| Multiple bands | isoforms 1 and 2 may differ in size, but distinct migration is unproven | check antibody specificity and compare relevant samples |
| Weak or no signal | secreted IL11 may be scarce in the sampled fraction | test conditioned medium and include a positive control |
Experiment evidence
Western blot images showing IL11 band
A01160 · IL11
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for IL11 Western blot
🧪For positive controls for IL11 in Western blot, you can use IL11-positive conditioned medium, since HPA provides no positive tissue or cell candidate.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: IL11 is secreted, so conditioned medium may give a clearer signal than whole-cell lysate; HPA provides no tissue control candidates.
HPA tissue expression evidence for IL11
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced IL11 Western Blot Tips
Deeper troubleshooting and optimisation questions for IL11, answered from its protein features.
How should IL11 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could IL11 isoforms produce different bands?
Isoforms · UniProt lists two isoforms. Isoform 2 lacks residues 1..79 of the canonical sequence, including the listed signal peptide. Their sequence lengths differ, but the features do not establish whether both isoforms are present in your sample or resolve as separate bands.
Do annotated modifications prove a band shift?
PTM · The linked UniProt record describes protein features. A modification annotation alone does not demonstrate a visible shift; retain any condition or experimental qualifier attached to it.
Does this guide establish induction of IL11?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for IL11 Western blot?
Transfer · IL11 is a relatively small protein, with a predicted mass of 21.4 kDa before signal-peptide removal. A 0.2 µm membrane is a practical choice to help retain it. Check transfer and retention with an appropriate protein standard; the features do not specify a transfer time.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A01160 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should IL11 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
Why might IL11 migrate below its predicted 21.4 kDa mass?
Interpretation · The 21.4 kDa prediction is for the 199-residue sequence. UniProt lists a signal peptide at residues 1..21, so secreted IL11 may lack that segment. No observed band position is supplied; signal-peptide processing alone does not establish an apparent mass.
IL11 is annotated as secreted. Check conditioned medium when assessing released IL11; a cell lysate alone may miss it. The supplied features do not specify how much IL11 is present in either fraction.
Because IL11 is secreted, compare equivalent conditioned-medium collection periods and account for differences in cell number or medium volume. Report which fraction was measured. The UniProt features provide no reference band or expected expression level.
Consider signal-peptide removal and the isoform 2 deletion of canonical residues 1..79 when evaluating lower bands. IL11 also associates with IL11RA and IL6ST in a signaling complex, but that annotation alone does not identify a higher band on a Western blot. Verify band identity experimentally.
IL11 is annotated as secreted. Check conditioned medium when assessing released IL11; a cell lysate alone may miss it. The supplied features do not specify how much IL11 is present in either fraction.
Because IL11 is secreted, compare equivalent conditioned-medium collection periods and account for differences in cell number or medium volume. Report which fraction was measured. The UniProt features provide no reference band or expected expression level.
Consider signal-peptide removal and the isoform 2 deletion of canonical residues 1..79 when evaluating lower bands. IL11 also associates with IL11RA and IL6ST in a signaling complex, but that annotation alone does not identify a higher band on a Western blot. Verify band identity experimentally.
Boster reagents
IL11 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-IL-11 Monoclonal Antibody
Cat # A01160
the supplier A01160 is an anti-IL-11 monoclonal antibody listed for human reactivity. Its supplied Western blot image uses truncated recombinant IL-11; no native tissue or cell sample evidence is provided.
Which to pick: A01160 is the only listed IL11 antibody and has a Western blot image using truncated recombinant IL-11. For native human samples, the supplied evidence does not show performance in that context.
Source: BosterBio IL11 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P20809.
- Human Protein Atlas. IL11 tissue expression.
- PMC13478155 — target-verified WB comparison
- PMC9603009 — target-verified WB comparison
- PMC8266813 — target-verified WB comparison