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- Table of Contents
4 Citations 14 Q&As
Facts about Interleukin-37.
Suppresses, or reduces, proinflammatory cytokine production, such as IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spares anti-inflammatory cytokines. Inhibits dendritic cell activation.
Human | |
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Gene Name: | IL37 |
Uniprot: | Q9NZH6 |
Entrez: | 27178 |
Belongs to: |
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IL-1 family |
FIL1 zeta; FIL1; FIL1(ZETA); FIL1ZFIL1 zeta; IL-1F7; IL-1F7IL-1 zeta; IL1H4IL1F7 (canonical product IL-1F7b); IL-1H4IL-1F7b (IL-1H4, IL-1H, IL-1RP1); IL-1RP1IL-1X protein; IL1RP1interleukin 1 family member 7; IL-1X; IL37; IL-37; interleukin 1 family, member 7 (zeta); interleukin 1, zeta; interleukin-1 family member 7; Interleukin-1 homolog 4; interleukin-1 superfamily z; Interleukin-1 zeta; Interleukin-1-related protein
Mass (kDA):
24.126 kDA
Human | |
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Location: | 2q14.1 |
Sequence: | 2; NC_000002.12 (112908885..112918882) |
In general, low constitutive expression, if any, in healthy tissues; high expression in inflammatory counterparts, including in synovial tissues from individuals with active rheumatoid arthritis. Isoform A, isoform B and isoform C are expressed in testis, colon, placenta, lung and lymph node. Isoform D and isoform E were found only in testis and bone marrow. Whereas only isoform A is found in brain, only isoform B in kidney and only isoform C in heart.
Cytoplasm, cytosol. Nucleus. Secreted. Stimulation with IL1B leads to colocalization with SMAD3 mostly in perinuclear regions (PubMed:20935647). Only the CASP1-cleaved mature form translocates into the nucleus upon LPS stimulation (PubMed:18390730).
You might have heard of the IL-37 marker , but might not know how to use it. We'll go over the many uses of the IL37 marker in this article. You'll learn the difference between the IL-37 marker and the IL-10, IL-6, and TNF-a markers. This article will also provide instructions on how to use the markers for your research.
IL-37 is an antibody that is found in cervical cancer tissues. Boster Bio's IL 37 mAb 1C6 recognizes the protein in human tissues. It has molecular masses of 42 kDa. It is highly specific for the endogenous human IL-37 that is expressed in cervical cancer tissue. Using this mAb, you can detect cervical cancer by comparing tumor samples to healthy tissue samples.
The IL-37 immunogen inhibits both adaptive as well in innate immune responses. It has important roles in the treatment of infectious and inflammatory conditions such as cancer and angiocardiopathy. The exact mechanisms that are involved in IL-37 regulation in human disease are not fully comprehended. In addition, the IL-37 mAb is highly specific to human IL-37b. While its role in these illnesses isn't yet clear but it is crucial for the study and understanding of numerous diseases.
Human IL-37b, which is a human antibody was created in the laboratory and then expressed on 293-T cells to test the antibody. In addition, a human IL37-GFP fusion protein was generated and was purified using a Protein G sepharose column. The results of this research revealed that IL37b-GFP fusion expression was high in 293-T cells.
The IL-10 marker is used to determine the presence of a variety of microorganisms that cause diseases. The bioactivity of the IL-10 marker is enhanced by linking it to the N-terminus of a different molecule. This can improve the stability and stability of the IL-10 dimer, which is a compound that is covalent with the helices A-D and E-F. It is also a method to determine the presence of IL-10 in a variety of cell types, including human asthma, lymphoma and inflammation-related diseases.
Numerous studies have demonstrated that IL-10 regulates the immune crosstalk between neurons and astrocytes in the CNS. Multiple sclerosis and Alzheimer's diseases are associated with a lack of IL-10 production. Researchers have also discovered that IL-10 signalling is impaired in Parkinson's disease. Further studies are needed to better be able to understand how this signalling regulated within the CNS.
The IL-10 marker stimulates the growth of spleen cells, in a dose-dependent fashion. Anti-IL-10 antibody (clone JES5-2A5) blocks phosphorylation STAT3 in cells. This signal is a sign of the presence of IL-10. After 30 minutes of incubation on ice the medium was added to 475mL spleen cell suspension.
The marker IL-6 is a vital cell cytokine. It plays vital roles in the acute phase reaction in inflammation, bone metabolism and the progression of cancer. The mature human version of the IL-6 marker is 183 amino acids long and shares 39% amino acids with IL-6 from mice and rats. Many of the isoforms show antagonistic properties. These can be used in research to develop more specific therapeutic approaches.
The cytokine IL-6 is a pre-inflammatory one produced by B and T lymphocytes. It increases within two hours of infection and peak four to six hours after infection. It is essential in evaluating the host's response to infection , as well as drug treatment. It is also used to detect intraamniotic infections. But these tests are not routinely performed clinically.
The signaling pathway for IL-6 is made up of two receptor chains. The membrane-bound IL-6R CD126 is bound to two molecules within gp130. These ligands activate downstream signaling pathways. Both types of IL-6R connect to Gp130, a protein that has the YxxQ motif. This molecule triggers the SHP2/Gab/MAPK signaling pathway.
The IL-6 hormone is often involved in inflammatory conditions, like rheumatoid or rheumatoid. It also has other biological effects , such as stimulation of RANKL which is an essential protein found in osteoclasts. Moreover, the IL-6 receptor stimulates RANKL which is responsible for the differentiation and activation of osteoclasts which is critical for bone resorption and osteoporosis.
This study investigates the potential role of IL37 in spontaneous preterm birth (sPTB). This inflammatory protein blocks the production of IL-1b, the IL-6, and TNF-a. It also regulates MMP9 which is an enzyme involved in the process of tissue repair. While this marker has a variety of applications, it is not clear exactly what it could do to help sPTB.
It is believed that IL-37 inhibits NF-kB signaling pathways and the IL-6/STAT3 signaling pathways. It also blocks apoptosis and participates in the remodeling of ECM. The IL-37 protein could play an important role in the prevention of premature births. Further research is required to determine its role in the preterm birth process.
This marker can be used to identify the presence of tumor-related inflammation. Through the use of siRNA to specifically target the IL-37 protein, researchers were able to study the effects of IL-37 on the death of cells. The study was conducted using 96-well plate plates, and samples were taken, and transfected over the course of a night with IL-37 siRNA. After 48 hours two probes with fluorescent light were added to each well and incubated for 15 minutes. Counts of dead cells were then taken.
The marker IL-37 is an important biomarker used to study the impact of IL 37 on cell growth and Apoptosis. To determine the IL-37 marker, human amniotic epithelial cells were treated with recombinant IL-37 protein or IL-6. Membrane tissues were extracted from RIPA lysis buffer, and the total protein concentration was measured by using the BCA Protein Assay Kit. The assay kit was designed and manufactured by Beyotime Biotechnology. Santa Cruz, USA and Servicebio Biotechnology provided the antibodies against IL-37, IL-6.
rhIL-337 markedly decreased the activity of MMP9, however, it had no effect on MMP2 in vitro. In in vitro, siRNA knockdown of IL37 dramatically increased MMP9 activity. However, it did not affect the levels of MMP2 protein. It is a protein that is expressed and controlled by the immune system in the body. It is a crucial marker in many pathologies.
The IL-37 receptor blocks apoptosis due to the regulation of two important proteins in the cell cycle, the antiapoptotic Bcl-2 molecule (proapoptotic molecule Bax), and the proapoptotic molecule Bax (proapoptotic molecule). Two proteins that IL37 regulates hinder apoptosis as well as limit the inflammatory response of cancer cells. Researchers can investigate the effects of IL37 on cancer by using the IL37 siRNA as biomarker.
The IL-6 hormone can also trigger an additional set of hepcidin genes in addition to the IL-6/STAT3 combo. This study was focused on IL-6-induced Hepcidin expression. The results revealed that hepcidin that was induced by IL-6 is enriched by a set of anti-STAT1 antibodies. The negative control primers amplify the region upstream of hepcidin promoter but don't contain a STAT consensus site. This strategy did not affect the recovery of immunoprecipitates.
Utilizing HepG2 cells, IL-6 and HyIL-6 were utilized to stimulate cells. Although HyIL-6 and IL-6 stimulations stimulated cells, they produced a brief effect. The IL-6/STAT3 responses were compared by intracellular flow cytometry. The IL-6/STAT3 signals were comparable regardless of the ratio of IL-6/STAT3.
In a study with humans, overexpression of IL-6 increased the growth of tumors and metastasis. In a murine model of breast cancer, an increase in the levels of IL-6 triggered tumor angiogenesis and the mobilization of meeloid cells that suppress tumors. Inhibition of Stat3 reduced the recruitment of MDSCs to the tumor stroma. Furthermore, lower levels of MDSCs reduced the burden of tumors.
IL-6and pStat3-expressing tumor cells spontaneously metastasize into the lung, whereas tumors that were injected only with pB did NOT cause lung metastases. Additionally, a two to threefold increase of tumor-derived and pStat3 in Boster Bio mice induced greater numbers of leukocytes expressing pStat3 and increased the number of endothelial cells.
PMID: 10744718 by Kumar S., et al. Identification and initial characterization of four novel members of the interleukin-1 family.
PMID: 11145836 by Pan G., et al. IL-1H, an interleukin 1-related protein that binds IL-18 receptor/IL- 1Rrp.
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