This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
1 Citations 10 Q&As
Facts about Interleukin-6 receptor subunit alpha.
Signal activation necessitate an association with IL6ST. Activation can lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
Human | |
---|---|
Gene Name: | IL6R |
Uniprot: | P08887 |
Entrez: | 3570 |
Belongs to: |
---|
type I cytokine receptor family |
CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL-6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor
Mass (kDA):
51.548 kDA
Human | |
---|---|
Location: | 1q21.3 |
Sequence: | 1; NC_000001.11 (154405193..154469450) |
Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
[Isoform 1]: Basolateral cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
This article will introduce the IL6R/rs2228145 marker as a potential exercise and physical activity marker. This marker has already been validated on WB and IHC. Here are some uses for this novel marker. They include: (i)
We have identified IL6R/rs222845 as a novel marker for inflammatory diseases. We observed that conditioned cell culture supernatants of PBMCs homozygous for the Asp358Ala IL-6R variant contained 2.5-fold more sIL-6R than did PBMCs from individuals homozygous for the common IL-6R variant. We do not yet know why this difference exists and whether the underlying genetic mechanism is a relevant one.
The sIL-6R and IL-6R/rs2228145 markers were found to be associated with sIL-6R levels, but the associations were not significant when the effect of rs2228145 was corrected. The sIL-6R/rs2228145 SNP was associated with higher levels of sIL-6R, while its expression level was unrelated.
GWAS and GCTA analysis revealed that IL6R/rs222845 is a novel marker for inflammatory diseases. The eQTL analysis of 4467 subjects identified 341 significant associations in which 5 probes measured the amount of IL6R RNA, four probes targeted the exon 7 region, and one probe measured the 3'end. Among the SNPs tested, rs7512646 had significant associations with the expression probe 582_132 and with all four exon 7 probes.
However, the genetic effects of IL6R/rs222845 on sIL-6R were not consistent. The effect of rs2228145 was only detected after the analysis of the a2residual and residual genetic effects. The effect of IL6R/rs2228145 on sIL-6R levels is additive, but does not explain the entire variance. Hence, IL6R/rs2228145 is still considered a novel marker.
The soluble form of IL-6R is widely expressed in body fluids, and it may act as a buffer for systemic IL-6 actions. A decrease in sIL-6R would lead to reduced CRP levels, while increased sgp130 would inhibit the trans-signaling of IL-6. However, both mechanisms may play a role. If one does not have a functional IL-6R, it is likely to have adverse effects on the immune system.
While immunohistochemistry is the preferred method of antibody validation, it is not always possible to validate an antibody on WB. In many cases, antibodies are used in studies without any sort of validation. Consequently, data generated with these antibodies are often flawed. A better method is to validate antibodies on IHC and WB, as well as by using knockout animals. The purpose of WB validation is to determine whether an antibody is specific or not.
The validation process involves analyzing the IHC staining of cancer cells for the presence of the IL6R antigen. The antibody used to detect the IL6R is made by Boster Bio and reacts with Human cells. This antibody is available for purchase in a range of concentrations and is stored at -20°C or 4°C. The antibody contains 5mg BSA and 0.05mg thimerosal. Boster Bio rewards the first reviewer with a product credit, and all scientists worldwide are eligible to participate.
In order to validate the IL6R marker on IHC, two pathologists independently performed IHC analysis of 155 patients with CRC. All samples were histopathologically confirmed. All patients provided written informed consent. The study protocol was approved by the Institutional Medical Ethics Committee of Xi'an Jiaotong University. This is a significant milestone for the IL6R on IHC.
Immunohistochemistry relies on the principle of binding of antibodies to antigens within cells. The method uses a host of techniques to visualize the binding of antibodies with antigens within biological tissues. Enzymes are commonly used to catalyze these reactions. It is important to understand the principle of immunohistochemistry before you begin any research project. The Boster Bio IL6R antibody is highly compatible with Boster's IHC protocol and can help you perform high-quality research.
The IL-6R antibody was able to detect IL-6R expression in CRC tumor cells. This marker was associated with the presence of integrin b6 and poor differentiation. It also correlated with the TNM and N stage of the tumor. Further, the IL-6R antibody also detected integrin b6 in stromal cells and tumor cells, and it is able to detect IL-6 and integrin b6 in cancer tissues.
Boster Bio IL6R is a chimeric antigen receptor (CAR) antibody that reacts with Human IL-6. It can be stored at -20deg C and is stable at 4deg C for one month. It contains 5mg BSA and 0.05mg thimerosal. The antiserum is available as an eluate or blocking peptide. The cost of the blocking peptide is determined by its length.
Boster Bio IL6R is validates on ICC, ELISA, and IP. It has been tested for use with Human. Its sensitivity has been verified using IP. The antibody comes in a 100uL liquid form and is tested in IHC and WB. It is available as a single or multiplex kit and is highly sensitive. If you have been unable to find a commercially available ELISA, this antibody is a good choice.
The antibody was confirmed to recognize human IL-6R, as shown by DLS analysis of the microvesicle suspension. It detected exosomes with hydrodynamic sizes of 19 nm, whereas the fraction at 100 nm corresponded to vesicle fragments isolated from the microvesicle enrichment procedure. The antibody recognizes the intracellular C terminus of human IL-6R. Interestingly, human IL-6R was not found in the HEK293 cells, indicating that the molecule is present.
The murine IL-6R gene consists of ten exons, including exon 9 that encodes the membrane-spanning region. The PCR product contains a 3'-primer located in the last exon and four different 5'-primers. A combination of these primers enables Boster Bio IL6R to identify human IL-6R. Once the protein is detected, the fragments are separated by agarose gel electrophoresis.
PMID: 3136546 by Yamasaki K., et al. Cloning and expression of the human interleukin-6 (BSF-2/IFN beta 2) receptor.
PMID: 1872801 by Schooltink H., et al. Structural and functional studies on the human hepatic interleukin-6 receptor. Molecular cloning and overexpression in HepG2 cells.
*More publications can be found for each product on its corresponding product page