Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™

Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL6R in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0777
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Product Name

Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™

See all Il6r products

SKU/Catalog Number

EK0777

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL6R in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0777)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-D358

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0777 is reactive to IL6R in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Application Guarantee

EK0777 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0777-CAP Anti-Human IL6R Pre-coated 96-well strip microplate 1
EK0777-ST Human IL6R Standard 2 vials, 10 ng/tube
EK0777-DA Human IL6R Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0120.1070.2000.3230.6941.1491.5212.126

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5427182159259773
Standard deviation2.8617.0741.873.7717.3544.06
CV (%)5.3%6.7%5.7%6.4%6.7%5.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Il6r in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1544854485135.8%
Sample 227125426223425513.665.3%
Sample 382192896587789754.216%
*number of samples for each test n=16.

Gene/Protein Information For IL6R (Source: Uniprot.Org, NCBI)

Uniprot ID

P08887

Gene ID

3570

Gene Name

IL6R

Full Name

Interleukin-6 receptor subunit alpha

Weight

51.548kDa

Superfamily

type I cytokine receptor family

Alternative Names

CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL-6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor IL6R CD126, HIES5, IL-1Ra, IL-6R, IL-6R-1, IL-6RA, IL6Q, IL6QTLA, IL6RQ, gp80, IL6R interleukin 6 receptor interleukin-6 receptor subunit alpha|CD126 antigen|IL-6 receptor subunit alpha|membrane glycoprotein 80

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL6R, check out the IL6R Infographic

IL6R infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL6R: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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10 Customer Q&As for Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-12-15

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-12-15

Question

Q: which procedure should I follow in order to thaw whole blood sample for Il6r ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-08-08

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Il6r for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-08-08

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-03-22

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-03-22

Question

Q: Can Il6r ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-05-16

Question

The protocol said 100µl/sample is needed to run the ELISA kit. However, the volumes of our samples are small and we are just wondering if 50µl/sample instead of 100µl/sample would work with the kit or not?

Verified customer

Asked: 2019-03-11

Answer

There are two solutions for insufficient sample volume for the Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™ (EK0777), 1.Diluent the samples with the provided sample diluent buffer into 100ul. 2.Add 50ul of standard solution as well, if 50ul of sample will be added into a well.

Boster Scientific Support

Answered: 2019-03-12

Question

Q: can you recommend the dilution ratio of serum samples for detection of Il6r in Human urine? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-05-29

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Il6r ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-05-29

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Il6r using the EK0777 Human Il6r Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-03-29

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2018-03-29

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2015-06-13

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2015-06-13

Question

Q: how do I analyze ELISA data? I measured Il6r level in urine.

Verified Customer

Verified customer

Asked: 2014-05-20

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2014-05-20

Question

Youngseok Song 6576 7243 I am a researcher from Singapore Eye Research Institute. I have a query regarding Human IL-6R Alpha / IL 6 Receptor PicoKine

Verified Customer

Verified customer

Asked: 2014-01-07

Answer

There are two solutions for insufficient sample volume. 1.Diluent the samples with the provided sample diluent buffer into 100ul. 2.Add 50ul of standard solution as well if 50ul of sample will be added into a well.

Boster Scientific Support

Answered: 2014-01-07

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