Overview
Product Name |
Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™
|
Catalog# |
EK0777 |
Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
Description |
Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL6R in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.. Sensitivity: 10pg/ml. |
Cite This Product |
Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0777)
|
ELISA Validation |
ELISA Validation Information
|
Product Specs
Reactivity/Species |
Human |
Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
Sample Type |
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. |
Sensitivity |
<10 pg/ml |
Assay Range |
31.2 pg/ml - 2,000 pg/ml |
Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: L20-D358 |
Antibody Clonalities |
Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For IL6R (Source: Uniprot.org, NCBI)
Uniprot Id | A0N0L5 |
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Gene Name | IL6R |
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Molecular Weight | 51548 MW |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IL6R, check out the IL6R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for IL6R: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the IL6R infographic
Kit Components And Assay QC Data Details Of Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
Catalog number | Description | Quantity |
EK0777-CAP |
Anti-Human IL6R Pre-coated 96-well strip microplate | 1 |
EK0777-ST | Human IL6R Standard | 2 vials, 10 ng/tube |
EK0777-DA | Human IL6R Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0777 Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.012 | 0.107 | 0.200 | 0.323 | 0.694 | 1.149 | 1.521 | 2.126 |
We measured random samples of Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 54 | 271 | 821 | 59 | 259 | 773 |
Standard deviation | 2.86 | 17.07 | 41.87 | 3.77 | 17.35 | 44.06 |
CV (%) | 5.3% | 6.7% | 5.7% | 6.4% | 6.7% | 5.7% |
We measured the performance consistency of Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|
Sample 1 | 54 | 48 | 54 | 48 | 51 | 3 | 5.8% |
Sample 2 | 271 | 254 | 262 | 234 | 255 | 13.66 | 5.3% |
Sample 3 | 821 | 928 | 965 | 877 | 897 | 54.21 | 6% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 0
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0777 Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™
10 Related Questions
Question
Youngseok Song
6576 7243
I am a researcher from Singapore Eye Research Institute.
I have a query regarding Human IL-6R Alpha / IL 6 Receptor PicoKine
Verified Customer
Asked: 2014-01-07
Answer
There are two solutions for insufficient sample volume.
1.Diluent the samples with the provided sample diluent buffer into 100ul.
2.Add 50ul of standard solution as well if 50ul of sample will be added into a well.
Boster Scientific Support
Answered: 2014-01-07
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Asked: 2020-12-15
Boster Scientific Support
Answered: 2020-12-15
Question
Q: which procedure should I follow in order to thaw whole blood sample for Il6r ELISA after freezing?
Verified Customer
Asked: 2020-08-08
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Il6r for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-08-08
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Asked: 2020-03-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-03-22
Question
Q: Can Il6r ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Asked: 2019-05-16
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-05-16
Question
Q: can you recommend the dilution ratio of serum samples for detection of Il6r in Human urine? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Asked: 2018-05-29
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Il6r ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-05-29
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Il6r using the EK0777 Human Il6r Picokine® ELISA Kit?
Verified Customer
Asked: 2018-03-29
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-03-29
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Asked: 2015-06-13
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2015-06-13
Question
Q: how do I analyze ELISA data? I measured Il6r level in urine.
Verified Customer
Asked: 2014-05-20
Boster Scientific Support
Answered: 2014-05-20
Question
The protocol said 100µl/sample is needed to run the ELISA kit. However, the volumes of our samples are small and we are just wondering if 50µl/sample instead of 100µl/sample would work with the kit or not?
Answer
There are two solutions for insufficient sample volume for the Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™ (EK0777),
1.Diluent the samples with the provided sample diluent buffer into 100ul.
2.Add 50ul of standard solution as well, if 50ul of sample will be added into a well.
Boster Scientific Support
Answered: 2019-03-12