Legumain Antibodies

AND LGMN ELISA kits, Legumain Proteins

Many biological assays use antibodies to detect Legumain, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Legumain in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Legumain antibodies...

We validate the specificity of these antibodies to Legumain by testing them on tissues known to express LGMN positively and negatively. Browse below to find the LGMN antibody that suites your experiment. We have 8 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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LGMN Infographic by Boster Bio

All LGMN Products

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Specificity

Applications

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Human Legumain(total) ELISA Kit PicoKine®

1 Citations 11 Q&As

Human

ELISA

$499
Cat # EK1566
96 wells/kit, with removable strips.

Mouse Legumain(total) ELISA Kit PicoKine®

1 Citations 8 Q&As

Mouse

ELISA

$499
Cat # EK1567
96 wells/kit, with removable strips.

Rat Legumain ELISA Kit PicoKine®

7 Q&As

Rat Legumain PicoKine ELISA Kit Standard Curve

Rat

ELISA

$499
Cat # EK2100
96 wells/kit, with removable strips.

Anti-Legumain LGMN Antibody

Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°over night

Human, Mouse, Rat

ELISA, WB

$380
Cat # A06476
100ul

Legumain (LGMN) (NM_005606) Human Recombinant Protein

$1249
Cat # PROTQ99538
20 µg

Mouse Legumain/Asparaginyl Endopeptidase Recombinant Protein

SDS-PAGE

$250
Cat # PROTO89017
Starting from 10ug

Anti-Legumain Rabbit Monoclonal Antibody

Western blot analysis of Legumain using anti-Legumain antibody (M06476)

Human, Mouse, Rat

IHC, WB

$370
Cat # M06476
100 μl/vial

Legumain (LGMN) (NM_005606) Human Over-expression Lysate

Legumain (LGMN) Human Over-expression Lysates NM_001008530

Human

$628
Cat # LS005641
20 µg, 100 µg

LGMN Overview

Facts about Legumain.

LGMN 3D structure. Source: alphafold

Legumain (LGMN)

Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions.

Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR.

Gene Information

Human
Gene Name:	LGMN
Uniprot:	Q99538
Entrez:	5641

Family

Belongs to:

peptidase C13 family

Alternative Names

AEP; Asparaginyl Endopeptidase; cysteine protease 1; Legumain; LGMN; LGMN1; Protease, cysteine 1; protease, cysteine, 1 (legumain); PRSC1EC 3.4.22.34

Molecular Weight

Mass (kDA):
49.411 kDA

Genomic Context


Human

Location:	14q32.12
Sequence:	14; NC_000014.9 (92703809..92748627, complement)

Tissue Specificity

Ubiquitous. Particularly abundant in kidney, heart and placenta.

Subcellular Localizations

Lysosome.

Diseases

Hyperdiploidy
Liver Carcinoma
Allelic Imbalance
Endometrial Polyp
Tissue Adhesions
Acute Lymphocytic Leukemia
Hepatocarcinogenesis
Diabetes Mellitus
Carcinoma
Carcinogenesis

Endometriosis, Site Unspecified
Exanthema
Leukemia
Cattle Diseases

Pathways

Cell Growth
Cell Adhesion
Regeneration
Cell Proliferation

PTMs

Disulfide bond

Glycoprotein

Zymogen

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/LGMN

References

PMID: 9065484 by Chen J.-M., et al. Cloning, isolation, and characterization of mammalian legumain, an asparaginyl endopeptidase.

PMID: 8893817 by Tanaka T., et al. Molecular cloning of a human cDNA encoding putative cysteine protease (PRSC1) and its chromosome assignment to 14q32.1.

1 Publications of Boster Legumain Antibodies & ELISA Kits

PubMed ID: 32229193, Chen HC,Rui W,You SY,Liu XW,Huang J,Chen HY.Evaluation of the anti-cervical canc...

*More publications can be found for each product on its corresponding product page