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- Table of Contents
Facts about Lutropin-choriogonadotropic hormone receptor.
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Human | |
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Gene Name: | LHCGR |
Uniprot: | P22888 |
Entrez: | 3973 |
Belongs to: |
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G-protein coupled receptor 1 family |
CGR; FLJ41504; HHG; hypergonadotropic hypogonadism; LCGR; LCGRLH/CGR; LGR2; LH/CG-R; LHCGR; LHR; LHRHR; LHRLGR2ULG5; LSH-R; Luteinizing hormone receptor; luteinizing hormone/choriogonadotropin receptor; lutropin/choriogonadotropin receptor; lutropin-choriogonadotropic hormone receptor; ULG5
Mass (kDA):
78.643 kDA
Human | |
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Location: | 2p16.3 |
Sequence: | 2; NC_000002.12 (48686774..48755724, complement) |
Gonadal and thyroid cells.
Cell membrane; Multi-pass membrane protein.
Boster has been the most renowned provider of laboratory test kits since. The kits can be used in the analysis of various biological samples. The TC5-SP5 confocal laser scanner is a well-liked choice for researchers looking to use the marker in studies involving the maturation of Oocytes as well as ovulation. Boster scientists are able to use the kit for these studies.
The Leica TC5/SP5 confocial Laser Scanning Microscope is an impressive spectral detector system. The five individually adjustable channels allow for spectral adjustment for all visible wavelengths. The detector optics cover the 400-800nm range. The scanner can be used in either resonant or conventional modes. The conventional mode can detect light at 10Hz to 2800Hz while the resonant mode can detect light at 8000Hz or 1600Hz.
This high-resolution confocal laser scanner can record images as a time-series. This is crucial in the fields of physiological and developmental biology which is where time-lapse experiments are essential to understand the processes of cells. Furthermore, this confocal lens can be used to capture images as they are captured. It supports high-resolution imaging, and can capture images that are up to 64 Megapixels.
The TC5 SP5 confocial scanning microscope features a pinhole that blocks out-of-focus light. The PMT is illuminated by light coming from the objective lens' focal plane. The light cuts through the sample. The images are clearer and display more detail. You can learn to create stereo images if are just beginning to learn about confocal microscopy.
The Laser scanner TC5SP5 confocalical microscope is equipped with a pinhole mechanism that selectively filters fluorescence signals from the sample area. The pinhole can also function as a lens, enabling an image that is planar of the area of the sample. This technique also improves the contrast of fluorescence in the image plane. For objects that are larger, the contrast of the fluorescence is significantly improved.
To determine the expression of L10P we tested it using an antibody specific to the C-terminal portion of LHCGR. Two bands with expected molecular sizes were observed in total homogenates derived from COS7 cells transfected by the WT receptor. The L10P mutation dramatically reduced the amount of receptor isoforms. We also utilized flow cytometry to measure the levels of LHCGR transcription.
The LHCGR gene is a big protein with several domains, one of which is the signal recognition particle (SRP). The SP has three subdomains: E, M Z, and E. The protein is crystallized from the L10P mutant with the presence of side-chains from Ala7 to Arg26. We utilized Sybyl-X 2.0 and PyMOL software to perform dynamic simulations of the protein's structure.
Total cell extracts were created by scraping cells into SDS-sample buffer, then heat-denaturing them. By plating identically many cells on a plate and executing loading tests, the cell DNA was the same in protein content. The proteins that resulted were separated by SDS-PAGE before being transferred to Hybond C-extra nitrocellulose. The antigen-antibody complexes could be detected by enhanced chemiluminescence.
Far-western blot is another method to identify LHCGR. This technique allows the detection of proteins that are identical to the target protein, without the need for an antibody. Alternately a probe that is 35S-labeled or in vitro translation reaction (IVTR) can be used to identify the binding partner. Biotin-binding protein conjugate can also be used for detection.
PI3K activation plays a crucial role in regulating LHCGR cell mRNA expression. FSH signals activate PI3K which is PKA dependent. It is possible that additional PKA-dependent pathways may influence LHCGR expression. We can't rule out these pathways, but we can eliminate the possibility that LHCGR does not have an effect on the metabolic process of the cell.
Boster Bio has developed the LHCGR marker to measure changes in the nuclear temperature during maturation of oocytes. These changes are correlated with various cellular processes and are useful for evaluating the oocytes' developmental capabilities. It can also help detect early failures to implant for women who haven't been able naturally to conceive.
Quality issues in oocytes are often due to spindle displacement, misalignment or excess cumulus cells. The presence of clear PB1 and the degree of attachment of cumulus cells are also indicative of oocyte quality. However, the attachment of cumulus cells is a key factor determining the quality of oocytes.
The LHCGR gene has been associated with the formation of primordial follicles. These follicles have a critical role in determining the fertility lifespan of both women and mammals. Oocytes in these follicles' primordial follicles are formed in the embryonic and prenatal stages. However, there are many unanswered questions in relation to the role played by external factors that influence the growth of oogonia, meiosis inhibition at metaphase I, as well as the breakdown of nests of oocytes.
PepT1 is an essential component of the LHCGR protein. When expressed in Xenopus levis Oocytes, it is a multimer. It regulates the expression of various Chemokines and cytokines. These include follicle-derived growth factors (FGF). It also regulates mTORC activity and may influence the design or new contraceptive agents.
The LHCGR (Luteinizing Hormone-Choriogonadotropin Receptor) is a member of the G protein-coupled receptor family. It has a large extracellular N-terminal domain as well as several leucine-rich repeats. It spans about 80 kb. It is mapped to 2p21 of chromosome 2. It is located in Leydig cells.
LHCGR (low -haplogroup, C-repeat, G-rich repeat) is a protein with three domains which include an extracellular (EC) transmembrane (TM) and a carboxyl tail. The EC domain has the N-terminal region of cysteine richness with a leucine-rich motif and a hinge zone. Below are three new variations of the LHCGR. A dark circle signifies amino acids that have been replaced in the coding sequence. A solid red circle denotes codons that stop. The yellow hexagonal forms denote glycosylation sites that are linked to N.
Previously, researchers were unsure of the association between LHCGR and pseudohermaphroditism. They discovered two mutations within LHCGR which led to an homozygous LHCGR mutation. The mutation changed a conserved cysteine gene to an arginine at codon 343 of the LHCGR gene. This study provides a link between LHCGR and male-limited precocious puberty and the results of this study.
Serum P and E2 levels are normal throughout the early and mid-follicular phases. LHCGR expression in the endometrium might increase during the time of implantation. This may prolong the period needed to conceive. In mice, the LHCGR expression increases during the window of implantation. High levels of hCG prolong the window for implantation and increase the likelihood of a successful pregnancy. This finding has important implications for the diagnosis and treatment of infertility.
Another possible cause is the production of testosterone that is excessive in the absence of stimuli to the testicle. The LHCGR gene is responsible for G protein binding. A mutation in this gene could result in the overproduction of testosterone in male-limited gonadotropin-independent precocious puberty. These results support the idea that LHCGR could be redundant in internalization. LHCGR is functional even if it does not reside on the membrane.
PMID: 2244890 by Minegish T., et al. Cloning and sequencing of human LH/hCG receptor cDNA.
PMID: 1922095 by Jia X.-C., et al. Expression of human luteinizing hormone (LH) receptor: interaction with LH and chorionic gonadotropin from human but not equine, rat, and ovine species.
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