Anti-hCG receptor/LHCGR Antibody
|Product Name||Anti-hCG receptor/LHCGR Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Lutropin-choriogonadotropic hormone receptor(LHCGR) detection. Tested with WB, IHC-P in Human;Rat.|
|Cite This Product||Anti-hCG receptor/LHCGR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1552)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human hCG receptor(127-143aa YLSICNTGIRKFPDVTK), different from the relative rat sequence by two amino acids, mouse sequence by three amino acids.|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human , By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-hCG receptor antibody, PA1552, IHC(P)
IHC(P): Rat Ovary Tissue
Anti-hCG receptor antibody, PA1552, Western blotting
All lanes: Anti hCG receptor (PA1552) at 0.5ug/ml
WB: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 78KD
Observed bind size: 78KD
Protein Target Info (Source: Uniprot.org)
|Protein Name||Lutropin-choriogonadotropic hormone receptor|
|Tissue Specificity||Gonadal and thyroid cells.|
|Alternative Names||Lutropin-choriogonadotropic hormone receptor;LH/CG-R;Luteinizing hormone receptor;LHR;LSH-R;LHCGR;LCGR, LGR2, LHRHR;|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein.|
|Molecular Weight||78643 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Receptor for lutropin-choriogonadotropic hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.|
|Research Areas||Human, Rat
*You can search these to find other products in these research areas.
|Background||The luteinizing hormone/choriogonadotropin receptor is a member of a subfamily of G protein-coupled receptors(GPCR) characterized by the presence of a large N-terminal extracellular domain containing several leucine-rich repeats(LRR). This glycoprotein hormone receptor family has been named the LRR-containing GPCR(LGR) family. The LHCGR gene contains 11 exons and spans approximately 80 kb and it is mapped to chromosome 2p21. In the ovary, theca, stromal, late-stage(luteinizing) granulosa, and luteal cells contain LHCGR. In the testes, Leydig cells contain LHCRG.|
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Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at firstname.lastname@example.org for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: What are your suggestions to reduce the background for my western blot? Keyword: troubleshooting, protocol supportA: Our technicians provide these suggestions to reduce the background: 1.Block the membrane 2 hours at room temperature with 5% no-fat milk in TBS-T; 2.Dilute second antibody with 5% no-fat milk in TBS-T; and 3.Extend the wash time to 10 minutes each time.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact email@example.com
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.