This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
LSM1 / U6 snRNA-associated Sm-like protein LSm1 · Western blot design guide
Design a Western Blot for LSM1
Source-linked LSM1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-LSM1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
LSM1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~15.2 kDa | |
| Gel | 15% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked LSM1 Western Blot Protocol Options
The A05573 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Hela, HEK293T (catalog A05573) |
| Gel % | 15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.2 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A05573; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected LSM1 Western Blot Band Size?
LSM1 is predicted at 15.2 kDa; its annotated phosphorylation sites may affect migration, but no empirical band size or visible shift is established.
| Single band near 15.2 kDa | Consistent with predicted LSM1 size; identity needs confirmation |
| Closely spaced bands near 15.2 kDa | Could reflect phosphorylation at Ser123 or Thr129, but separation is unproven |
| Band near 15.2 kDa in whole-cell lysate | Consistent with cytoplasmic LSM1 |
| No band near 15.2 kDa in whole-cell lysate | Cytoplasmic localization does not explain its absence; check detection and identity |
💡Expected LSM1 appearanceUniProt predicts 15.2 kDa for LSM1, but no empirical band size is supplied; use a band near that size as a starting point and confirm its identity with controls.
| UniProt predicted mass | Places the expected band near 15.2 kDa |
| Phosphoserine at Ser123 | A distinct apparent size shift is not established |
| Phosphothreonine at Thr129 | A distinct apparent size shift is not established |
| Cytoplasmic P-body localization | Does not establish an apparent size change |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Cytoplasmic localization does not account for absence from whole-cell lysate | Check loading and antibody detection; use an LSM1 depletion control |
| Band higher than expected | No supplied feature establishes a large upward shift | Check molecular-weight calibration and test band identity by LSM1 depletion |
| Band lower than expected | No signal peptide or propeptide cleavage is annotated | Check sample integrity and test band identity by LSM1 depletion |
| Multiple bands | Phosphorylation at Ser123 or Thr129 could contribute, but distinct bands are unproven | Compare phosphatase-treated material and an LSM1 depletion control |
| Weak or no signal | The supplied features do not establish a cause | Check loading, transfer, and antibody detection with appropriate controls |
Experiment evidence
Western blot images showing LSM1 band
A05573 · LSM1
A05573-1 · LSM1
A05573-2 · LSM1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for LSM1 Western blot
🧪For positive controls for LSM1 in Western blot, you can use a validated positive sample once one is identified; the supplied HPA evidence names none.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA provides no tissue controls, so validate a cytoplasmic lysate signal using siRNA knockdown or a KO line.
HPA tissue expression evidence for LSM1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced LSM1 Western Blot Tips
Deeper troubleshooting and optimisation questions for LSM1, answered from its protein features.
How should LSM1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could an annotated LSM1 isoform explain a second band?
Isoforms · Only one isoform is listed, with no alternative sequence. A second band therefore cannot be assigned to an annotated isoform from these features alone.
Which LSM1 phosphorylation sites matter when interpreting bands?
PTM · UniProt lists phosphoserine at position 123 and phosphothreonine at position 129. Keep those UniProt coordinates when comparing antibody or paper numbering. Neither site alone demonstrates a resolvable band shift.
Quantify total LSM1 and any site-specific phosphorylation signal separately. For a phospho-specific comparison, relate that signal to total LSM1 in the same conditions; the listed sites at UniProt positions 123 and 129 do not establish phosphorylation occupancy or changes in protein abundance.
Quantify total LSM1 and any site-specific phosphorylation signal separately. For a phospho-specific comparison, relate that signal to total LSM1 in the same conditions; the listed sites at UniProt positions 123 and 129 do not establish phosphorylation occupancy or changes in protein abundance.
Does this guide establish induction of LSM1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for LSM1 Western blot?
Transfer · LSM1 is predicted to be 15.2 kDa. Check that the transfer retains and detects protein near this size. The supplied features do not identify a preferred transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A05573 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should LSM1 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
Should LSM1 migrate exactly at its predicted 15.2 kDa?
Interpretation · Use 15.2 kDa as the sequence-based reference, not an exact migration requirement. No observed band size is supplied, and the listed features do not establish a visible shift or explain any difference from the predicted mass.
The supplied feature says LSM1 interacts with SLBP when histone mRNA is rapidly degraded during S phase. It does not establish increased LSM1 abundance. If comparing cell-cycle conditions, measure LSM1 signal rather than treating the interaction as evidence of induction.
The features list phosphorylation at UniProt positions 123 and 129 and interactions with SLBP and other LSm subunits. They do not show that these features produce additional Western blot bands. Compare unexpected signals with the 15.2 kDa reference and validate their LSM1 identity before assigning a cause.
The supplied feature says LSM1 interacts with SLBP when histone mRNA is rapidly degraded during S phase. It does not establish increased LSM1 abundance. If comparing cell-cycle conditions, measure LSM1 signal rather than treating the interaction as evidence of induction.
The features list phosphorylation at UniProt positions 123 and 129 and interactions with SLBP and other LSm subunits. They do not show that these features produce additional Western blot bands. Compare unexpected signals with the 15.2 kDa reference and validate their LSM1 identity before assigning a cause.
Boster reagents
LSM1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-LSM1 Antibody
Cat # A05573
Real WB data
Anti-LSM1 Antibody
Cat # A05573-1
Real WB data
Anti-LSM1 Antibody
Cat # A05573-2
Three anti-LSM1 antibodies have WB images. A05573 shows HeLa and HEK293T lysates at 1:500; A05573-2 shows K562 lysate at 1:1000. A05573-1 reports various cell lines at 1:3000 without naming them. These captions do not establish mouse WB validation.
Which to pick: For HeLa or HEK293T, consider A05573; for K562, consider A05573-2. All three have WB images. A05573-1 and A05573-2 list Human and Mouse reactivity, but their supplied captions do not identify a mouse sample.
Source: BosterBio LSM1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry O15116.
- Human Protein Atlas. LSM1 tissue expression.
- PMC3545066 — target-verified WB comparison
- PMC10635750 — target-verified WB comparison