LTK Antibodies

Leukocyte tyrosine kinase receptor (LTK)

Receptor using a tyrosine-protein kinase activity. The exact function of this protein is not known.

Studies with chimeric proteins (substituting its extracellular region with that of several known growth factor receptors, such as EGFR and CSFIR) demonstrate its ability to promote growth and specifically neurite outgrowth, and cell survival. Signaling appears to involve the PI3 kinase pathway.

Gene Information

Human
Gene Name:	LTK
Uniprot:	P29376
Entrez:	4058

Family

Belongs to:
protein kinase superfamily

Alternative Names

EC 2.7.10.1; leukocyte receptor tyrosine kinase; leukocyte tyrosine kinase receptor; leukocyte tyrosine kinase; LTK; TYK1; TYK1Protein tyrosine kinase 1

Molecular Weight

Mass (kDA):
91.681 kDA

Genomic Context

Human
Location:	15q15.1
Sequence:	15; NC_000015.10 (41503637..41513887, complement)

Tissue Specificity

Expressed in non-hematopoietic cell lines and T- and B-cell lines.

Subcellular Localizations

Membrane; Single-pass type I membrane protein.

Diseases

• Corneal Diseases
• Hyperopia
• Lymphoma
• Refractive Errors
• Myopia
• Anaplastic Lymphoma
• Nervousness
• Malignant Neoplasms
• Neoplasms
• Parkinson Disease
• Malignant Paraganglionic Neoplasm
• Chromosomal Translocation

• Neuroblastoma
• Hyperplasia
• T(2;5)(p23;q35)
• Inflammation
• Hypoxia
• Patient Dependence On

Interacting Proteins

• ALKAL1

Pathways

• Coagulation
• Localization
• Cell Growth
• Pathogenesis
• Transport
• Translation
• Wound Healing
• Proteolysis
• Gene Conversion
• Sensitization
• System Development
• Vasoconstriction
• Dopamine Uptake

• Methylation
• Cell Death
• Secretion
• Protein Phosphorylation
• Endoplasmic Reticulum Localization
• Hyperphosphorylation
• Regulation Of Signaling

PTMs

• Phosphorylation
• Glycosylation
• Biotinylation
• Cleavage

• Methylation
• Reduction
• Dephosphorylation
• Ribosylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/LTK

Boster Bio: Best Uses Of The LTK Marker

The LTK marker is a highly specific protein and the Boster company's antibodies have been validated by multiple platforms. Boster also validates antibodies with known positive samples and negative samples. They also reward scientists for the first product reviews. Boster will provide product credits as a reward for you reviewing one of their products. The company also rewards scientists all over the world for reviewing its products.

Anti-LTK Antibody

Boster Bio makes antibodies that react with human leukocyte tyrosine kinase (LTK) or human LTK. These antibodies are highly specific and affinity-sensitive and have a 25-year history of reliable performance. To further increase the quality of their antibodies, they rigorously test them on Western Blotting, Immunohistochemistry, and ELISA platforms.

Primary antibodies with high-affinity

Boster offers a complete array of ELISA products and high-affinity, primary antibodies for your research. Their antibody kits detect biomarkers related to cancer, neurosciences and development and can reach picogram-level sensitivities. All their immunological agents are made in house under strict quality control. You can order these products through tebu-bio, a division of Boster Bio.

Flow cytometry has countless applications in science. It measures a sample's response to a particular antigen. Boster Bio's high-affinity primary antibody is both monoclonal (polyclonal) and multiclonal (monoclonal). Their high affinity antibodies have been highly cited over the last 25 years and continue to be so. You can trust that they will provide the best results for your research. Flow cytometry is a complex analytical method with numerous applications. Flow cytometry is a powerful analytical method that can be used to conduct successful experiments.

Boster offers a complete line of reagents to be used in PCR. Their kits detect hormones small molecules, DNA damage and oxidative stresses. The kits come with enough reagents to run 96 tests. Boster also offers convenient solutions for PCR. You can use their ELISA kit with a variety sample types. You can choose between a variety if samples and then decide which one suits your research best.

B cells that recognize the antigen are more likely to respond during the initial phase. During the second response, the IgG antibodies are produced by the B cells. The rest of the cells, including memory, then differentiate to produce IgA, IgE antibodies. The body can effectively fight off pathogens by producing antibodies that are highly specific for their antigens in the primary response.

Validation of WB

Bosterbio should always generate the same final concentrations of the analyte using the dilution sequence. This is known as linearity and is critical to reliable results. Boster Bio generates the dilution series within the dynamic range for the assay. For this validation, a serum sample of a healthy human was serially diluted to obtain values that were within the dynamic range of the assay.

The company has been producing antibodies for over 25 year and has developed ELISA Kits for biomarkers within neurosciences, developmental biology and inflammation. The Boster ELISA kits are available at tebu bio and reach picogram levels. For accurate results, researchers can consult the ELISA protocol as well as images provided by the company. These images can be downloaded free of charge. Supervision, a polymer-based secondary antigen, saves significant time in IHC. Boster Bio's ELISA kits come with technical support from Sanbio, a BeNeLux distributor.

Immunohistochemistry, a technique for staining cells and detecting antigens within cells, is a unique technique. Boster Bio optimises the antibodies and dilution ratio for each target and offers Multiplex Immunohistochemistry services. This service allows you to detect multiple targets in one assay while ensuring high throughput. Assay processing time is also faster with Multiplex Immunohistochemistry.

Validation at ICC

Immunocytochemistry (ICC), is used to determine the presence of antigens in a sample. This is a widely used clinical laboratory test, and is characterized by its low sensitivity, which may lead to inaccurate results. In contrast, molecular pathology techniques, such as LTK markers, are more specific and robust. Immunocytochemistry must be validated just as rigorously as molecular pathway techniques. This validation protocol provides a three-step process that emphasizes the therapeutic potential of ICC.

Validation on Immunofluorescence

Antibody-based immunofluorescence tests are a significant investment in time and money. However, there is no standard to determine what constitutes valid antibody validation. Many commercial antibodies are not validated. There are also no experimental data. Poor antibody validation has caused a loss of time and money as it has not been possible to reproduce the results. It is essential to establish a standard set of guidelines to ensure high-quality antibodies.

The level of antigenicity will dictate the detection method that is used. The sensitivity of Labelled StreptAvidin Biologic (LSAB) methods may not be sufficient to detect low levels. Polymer systems or tyramine amplification might be necessary. Esoteric methods may lead to inconsistency in staining results, so variation should be systematically approached. It is important that staining results are recorded using a standard reporting template. This will allow for comparisons between runs.

The Uhlen group has developed a stepwise protocol for assessing cancer-related biomarkers in formalin fixed paraffin-embedded tumor specimens. Although it does not allow for scientific rigor and lab economics, the stepwise approach could be applied to other antibody based assays. This protocol can be used to validate antibodies for VEGF. These steps will enable researchers to determine whether an antibody is suitable for cancer-related biomarkers.

Mass spectrometry (MS), is an advanced technique that can determine antibody quality and specificity. It is especially sensitive to amino acid-modified antibodies. Two MS-based methods have been created for this purpose. The first method scores the quality of antibodies using 1,124 recombinant antibody, while the other uses 152 chromatin related proteins. It is important for you to know that mass-spectrometry is a sensitive and specific technique to determine the quality of antibodies for immunoprecipitation.