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Product Info Summary
| SKU: | PA1990 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-LTK Antibody Picoband®
SKU/Catalog Number
PA1990
BA3092-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LTK Antibody catalog # PA1990. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LTK Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1990)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human LTK.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1990 is reactive to LTK in Human
Observed Molecular Weight
85-91 kDa
Calculated molecular weight
91.7 kDa
Background of LTK
LTK (Leukocyte Tyrosine Kinase), also known as TYK1, is an enzyme that in humans is encoded by the LTK gene. The protein encoded by this gene is a member of the ALK/LTK receptor family of receptor tyrosine kinases (RTKs) whose ligand is unknown. Toyoshima et al. (1993) cloned a set of cDNAs representing differently spliced human LTK mRNAs. Liao et al. (1996) found that the mouse Ltk gene is closely linked to the Tyro3 gene which maps to mouse chromosome 2. Thus, the LTK gene produces not only the putative receptor tyrosine kinase for an unknown ligand but also multiple protein products that may have different functions.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1990 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human THP-1 whole cell, human Caco-2 whole cell, human K562 whole cell, human Jurkat whole cell, human MDA-MB-453 whole cell, human U2OS whole cell, human PC-3 whole cell,, rat thymus tissue, rat brain tissue, mouse brain tissue,
Validation Images & Assay Conditions
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Western blot analysis of LTK using anti-LTK antibody (PA1990).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates
Lane 2: human Caco-2 whole cell lysates
Lane 3: human K562 whole cell lysates
Lane 4: human Jurkat whole cell lysates
Lane 5: human MDA-MB-453 whole cell lysates
Lane 6: human U2OS whole cell lysates
Lane 7: human PC-3 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LTK antigen affinity purified polyclonal antibody (Catalog # PA1990) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LTK at approximately 85-91KD. The expected band size for LTK is at 91KD.
Click image to see more details
Western blot analysis of LTK using anti-LTK antibody (PA1990).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates
Lane 2: rat brain tissue lysates
Lane 3: mouse brain tissue lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LTK antigen affinity purified polyclonal antibody (Catalog # PA1990) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LTK at approximately 85-91KD. The expected band size for LTK is at 91KD.
Specific Publications For Anti-LTK Antibody Picoband® (PA1990)
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3 Customer Q&As for Anti-LTK Antibody Picoband®
Question
I was wanting to use to test anti-LTK antibody PA1990 on human mucosa of transverse colon for research purposes, then I may be interested in using anti-LTK antibody PA1990 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
The products we sell, including anti-LTK antibody PA1990, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-13
Question
Would anti-LTK antibody PA1990 work on goat WB with tongue?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
Our lab technicians have not tested anti-LTK antibody PA1990 on goat. You can run a BLAST between goat and the immunogen sequence of anti-LTK antibody PA1990 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat tongue in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-20
Question
We are currently using anti-LTK antibody PA1990 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
The anti-LTK antibody (PA1990) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-11
