Anti-LTK Antibody

Product Name

Anti-LTK Antibody

View all LTK Antibodies

SKU/Catalog Number

PA1990

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-LTK Antibody catalog # PA1990. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LTK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1990)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human LTK.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1990 is reactive to LTK in Human

Applications

PA1990 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

85-91 kDa

Calculated molecular weight

91.681kDa

Background of LTK

LTK (Leukocyte Tyrosine Kinase), also known as TYK1, is an enzyme that in humans is encoded by the LTK gene. The protein encoded by this gene is a member of the ALK/LTK receptor family of receptor tyrosine kinases (RTKs) whose ligand is unknown. Toyoshima et al. (1993) cloned a set of cDNAs representing differently spliced human LTK mRNAs. Liao et al. (1996) found that the mouse Ltk gene is closely linked to the Tyro3 gene which maps to mouse chromosome 2. Thus, the LTK gene produces not only the putative receptor tyrosine kinase for an unknown ligand but also multiple protein products that may have different functions.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of LTK using anti-LTK antibody (PA1990).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates
Lane 2: human Caco-2 whole cell lysates
Lane 3: human K562 whole cell lysates
Lane 4: human Jurkat whole cell lysates
Lane 5: human MDA-MB-453 whole cell lysates
Lane 6: human U2OS whole cell lysates
Lane 7: human PC-3 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LTK antigen affinity purified polyclonal antibody (Catalog # PA1990) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LTK at approximately 85-91KD. The expected band size for LTK is at 91KD.

Click image to see more details

Figure 2. Western blot analysis of LTK using anti-LTK antibody (PA1990).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates
Lane 2: rat brain tissue lysates
Lane 3: mouse brain tissue lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LTK antigen affinity purified polyclonal antibody (Catalog # PA1990) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LTK at approximately 85-91KD. The expected band size for LTK is at 91KD.

Gene/Protein Information For LTK (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.10.1; leukocyte receptor tyrosine kinase; leukocyte tyrosine kinase receptor; leukocyte tyrosine kinase; LTK; TYK1; TYK1Protein tyrosine kinase 1 LTK TYK1 leukocyte receptor tyrosine kinase leukocyte tyrosine kinase receptor|protein tyrosine kinase 1

For more info on LTK, check out the LTK Infographic

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In this infographic, you will see the following information for LTK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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