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MCM7 / DNA replication licensing factor MCM7 · IHC design guide
Design Immunohistochemistry for MCM7
Plan MCM7 chromogenic IHC around nuclear staining in highly proliferative cells (HPA tissue IHC). This guide covers paraffin-section controls, consistent fixation, antibody dilution, detection, counterstaining and nuclear scoring (standard IHC practice).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
MCM7 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Nuclear staining observed in proliferative cells (HPA tissue IHC) | |
| Staining pattern | Nuclear staining in highly proliferative cells (HPA tissue IHC) | |
| Antigen retrieval | EDTA pH 8.0 HIER, heat-mediated (datasheet PB9261) | |
| Positive control | Appendix+4 more · see all | |
| Negative control | Adipose tissue+4 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Keep fixation consistent across sections (standard IHC practice; not target-specific) | |
| Caveat | Proliferation differences can change the stained-cell fraction (HPA tissue IHC) | |
| Regulation | Enriched in highly proliferative cells (HPA tissue IHC) | |
| Isoform / epitope | Three isoforms; epitope coverage is unspecified (UniProt) |
Section 1
Recommended MCM7 IHC & IF Protocols
The catalog antibody uses EDTA pH 8.0 heat retrieval (datasheet: PB9261). Four published MCM7 IHC protocols provide additional conditions (PMC3964599; PMC3125391; PMC4156209; PMC5250507).
| Sample | Paraffin-embedded Mouse Testis tissue; fixative not specified (datasheet PB9261) |
| Fixation | Image fixative and duration unreported (datasheet PB9261); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: EDTA pH 8.0 (datasheet PB9261); 20 min, 95–100 °C (standard) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% goat serum (datasheet PB9261) |
| Primary antibody | Rabbit anti-MCM7, 0.5-1μg/ml (datasheet PB9261) |
| Primary incubation | Overnight at 4 °C (datasheet PB9261) |
| Detection | Streptavidin-biotin complex (SABC), DAB chromogen (datasheet PB9261) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | MCM7-positive staining in endocrine cells of appendix (HPA tissue IHC: High). HPA tissue profile: Nuclear expression in highly proliferative cells. No signal in the no-primary control. |
💡Decision noteStart with EDTA pH 8.0 heat retrieval for the catalog antibody (datasheet: PB9261). Citrate pH 6.0 is a published alternative for NSCLC sections (PMC4156209).
Section 2
What Is the Expected MCM7 Staining Pattern?
MCM7 should stain nuclei, chiefly in highly proliferative cells (HPA tissue IHC: nuclear expression in highly proliferative cells; UniProt P33993: nucleus and chromosome). Expect strong staining in the specific cell populations HPA rates High, such as bone marrow hematopoietic cells and lymph node germinal center cells (HPA tissue IHC: High). HPA rates the tissue pattern Enhanced, reflecting high consistency between antibody staining and RNA expression data (HPA tissue IHC: Enhanced).
| Strong nuclear staining in bone marrow hematopoietic cells or lymph node germinal center cells. | This matches the expected compartment and two High-rated cell populations (UniProt P33993: nucleus; HPA tissue IHC: High in these cells). Score the proportion and intensity of stained nuclei within the relevant population; a positive field need not mean every cell is positive (standard IHC practice). |
| Predominantly cytoplasmic staining, with little nuclear signal, in an otherwise positive field. | Treat this as an unexpected IHC pattern and review specificity and detection controls (UniProt P33993: nucleus; HPA tissue IHC: nuclear expression). HPA also reports cytosol and basal body in ICC-IF; those approved additional locations do not establish a predominantly cytoplasmic chromogenic tissue pattern (HPA subcellular ICC-IF). |
| Strong staining in adipocytes or cardiomyocytes, especially without corresponding positive nuclei. | These cells are rated Not detected, so investigate cross-reactivity or nonspecific detection before interpreting the stain as MCM7 (HPA tissue IHC: Not detected in adipocytes and cardiomyocytes). Compare cell identity and compartment on the same section and with appropriate controls (standard IHC practice). |
| Diffuse color over nuclei, cytoplasm, and tissue spaces, obscuring cell boundaries. | This cannot be scored as the expected nuclear pattern (HPA tissue IHC: nuclear expression). Background from detection reagents, insufficient blocking, or inadequate washing is possible; use a no-primary control to help identify reagent-related staining (standard IHC practice). |
| No nuclear staining in bone marrow hematopoietic cells despite intact tissue morphology. | A High-rated reference population has failed to stain, so the result is inconclusive until the assay is checked (HPA tissue IHC: High in bone marrow hematopoietic cells). Examine the IHC-validated antibody, retrieval and detection workflow, and a positive control processed alongside the section (standard IHC practice). |
💡Expected MCM7 appearanceCall the stain positive when distinct, often strong nuclear signal marks the specified High-rated cell populations; dominant cytoplasmic color or strong staining of Not detected populations is suspect (UniProt P33993: nucleus; HPA tissue IHC: High and Not detected populations).
| Compartment and replication state | MCM7 is nuclear and chromosome associated; it associates with chromatin before nuclei form and detaches as DNA replication progresses (UniProt P33993). These facts support nuclear interpretation but do not define a slide-based cell-cycle cutoff or require every nucleus to stain (UniProt P33993; HPA tissue IHC: nuclear expression). |
| Cell population within a tissue | HPA rates bronchial basal cells, esophageal squamous epithelial cells, and appendix endocrine cells High, while adipocytes and cardiomyocytes are Not detected (HPA tissue IHC). Identify the cell population before calling a tissue positive or negative; an organ-wide label would obscure these distinctions (HPA tissue IHC). |
| Strength of IHC evidence | The tissue pattern is Enhanced, with high consistency between staining and RNA expression (HPA tissue IHC: Enhanced). HPA lists HPA003898, CAB002163, and CAB016312 as IHC Enhanced; that validation supports their reported patterns but does not validate an unlisted antibody or a particular staining run (HPA antibodies). |
| ICC-IF localization boundary | HPA approves nucleoplasm as the main ICC-IF location and cytosol and basal body as additional locations (HPA subcellular ICC-IF). This is a useful answer when comparing modalities, but ICC-IF observations do not supply a chromogenic IHC protocol or override the tissue IHC nuclear profile (HPA subcellular ICC-IF; HPA tissue IHC). |
| Protein architecture and variants | UniProt lists no transmembrane segment or signal peptide, three isoforms, and an MCM C-terminal AAA(+) ATPase domain at residues 316–647 (UniProt P33993). These annotations do not identify the catalog antibody's epitope or predict its retrieval needs; confirm those assay details from the antibody's own documentation (standard IHC practice). |
| Target-specific fixation sensitivity | No target-specific fixation effect is established by the supplied UniProt localization or HPA staining data (UniProt P33993; HPA tissue IHC). If retrieval needs adjustment, treat it as assay optimization and judge the result against positive and negative control tissues, rather than assuming a documented MCM7 fixation response (standard IHC practice). |
| Situation | Likely cause | Next action |
|---|---|---|
| Known-positive tissue has no nuclear signal. | The staining run may have failed; bone marrow hematopoietic cells and lymph node germinal center cells are High-rated references (HPA tissue IHC). | Check that a matched positive control stained, then review the IHC-validated antibody, retrieval, dilution, and detection steps under the assay documentation (standard IHC practice). Do not score the test section as biologically negative until the control works. |
| Signal is weak in a population expected to be High. | Weak detection or an unsuitable assay setting is possible; HPA's High category describes the observed reference pattern, not a guaranteed intensity in every run (HPA tissue IHC: High). | Compare morphology and a concurrently processed positive control, then adjust one documented assay variable at a time and reassess nuclear contrast (standard IHC practice). |
| Color is mainly cytoplasmic. | The result conflicts with the tissue IHC nuclear profile; ICC-IF's additional cytosol location alone cannot establish this chromogenic pattern (HPA tissue IHC: nuclear expression; HPA subcellular ICC-IF: cytosol). | Review the no-primary control, cell boundaries, and antibody specificity; accept MCM7 IHC only when the expected nuclear pattern is demonstrable in a suitable positive population (standard IHC practice; HPA tissue IHC). |
| Adipocytes or cardiomyocytes stain strongly. | Those populations are Not detected in the HPA tissue profile; cross-reactivity or nonspecific chromogen is possible (HPA tissue IHC: Not detected). | Confirm cell identity and compare a no-primary control with a positive reference on the same run (standard IHC practice). If unexpected staining persists only with primary antibody, reassess specificity before reporting it as MCM7. |
| Diffuse background obscures positive nuclei. | Detection reagent background, insufficient blocking, or inadequate washing can reduce interpretability (standard IHC practice). | Inspect a no-primary control, then review blocking and wash steps and the detection system (standard IHC practice). Reassess whether discrete nuclear staining remains in High-rated cells (HPA tissue IHC: nuclear expression and High populations). |
| A section seems negative because most cells are unstained. | HPA assigns staining levels to named cell populations, not every cell in an organ; it also lists several populations as Not detected (HPA tissue IHC). | Evaluate the relevant cell type and nuclear compartment separately, using morphology and a working positive control (standard IHC practice; HPA tissue IHC: nuclear expression). Report the observed population and pattern rather than an organ-wide positive or negative call. |
Experiment evidence
IHC & IF images showing MCM7 staining
PB9261 · IHCIHC analysis of MCM7 using anti-MCM7 antibody (PB9261). MCM7 was detected in a paraffin-embedded section of Mouse Testis tissue. Heat mediat
PB9261 · IHCIHC analysis of MCM7 using anti-MCM7 antibody (PB9261). MCM7 was detected in a paraffin-embedded section of Mouse Testis tissue. Heat mediat
PB9261 · IFIF analysis of MCM7 using anti-MCM7 antibody (PB9261). MCM7 was detected in immunocytochemical section of U20S cells. Enzyme antigen retriev
M01649-3 · IHCIHC analysis of MCM7 using anti-MCM7 antibody (M01649-3). MCM7 was detected in a paraffin-embedded section of human laryngeal squamous cell
M01649-3 · IHCIHC analysis of MCM7 using anti-MCM7 antibody (M01649-3). MCM7 was detected in a paraffin-embedded section of human adenocarcinoma of the co
M01649-3 · IFIF analysis of MCM7 using anti-MCM7 antibody (M01649-3). MCM7 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen re
PA1792 · IHCIHC analysis of MCM7 using anti-MCM7 antibody (PA1792). MCM7 was detected in a paraffin-embedded section of human bladder cancer tissue. Hea
PA1792 · IHCIHC analysis of MCM7 using anti-MCM7 antibody (PA1792). MCM7 was detected in a paraffin-embedded section of human ovarian serous adenocarcin
PA1792 · IFIF analysis of MCM7 using anti-MCM7 antibody (PA1792) and anti-Tubulin Alpha antibody (M03989-3). MCM7 was detected in immunocytochemical se
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000166508-MCM7/tissue/cerebral+cortex#imid_4208940
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000166508-MCM7/tissue/colon#imid_4208874
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000166508-MCM7/tissue/liver#imid_4208883
HPA · KidneyHuman Protein Atlas IHC, Kidney. Source: https://www.proteinatlas.org/ENSG00000166508-MCM7/tissue/kidney#imid_4208884
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody CAB016312; green = MCM7, blue = nucleus, red = microtubules.
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody CAB016312; green = MCM7, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for MCM7 IHC & IF
🧪Run bone marrow first: hematopoietic cell nuclei should stain (HPA: High in bone marrow hematopoietic cells). Use adipose tissue as the negative tissue, focusing on adipocytes (HPA: Not detected in adipocytes); on the positive slide, treat nuclei without specific chromogen as internal background controls, without assuming a particular marrow cell population is MCM7-negative (standard IHC practice).
Positive control tissue: Appendix (Endocrine cells, HPA High)
Negative control tissue: Adipose tissue (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show MCM7 in A-431, U-251MG, U2OS, MCF-7, ASC52telo, RPTEC/TERT1, hTERT-RPE1 (serum starved), with annotated localisation: Nucleoplasm (approved) (HPA subcellular).
Technical controls: Include a no-primary, secondary-only control and a concentration-matched rabbit IgG isotype control for the rabbit primary (caption: rabbit anti-MCM7; standard IHC practice). Confirm specificity with MCM7 knockout tissue or a peptide-block control if a matching immunizing peptide is available; in bone marrow, quench endogenous peroxidase and check endogenous biotin when using SABC/DAB detection (caption: SABC/DAB; standard IHC practice).
⚠️Feasibility: A target-specific fixation window and fixation effect are unreported, and the selected PB9261 paraffin-section caption does not state the fixative (selected-SKU caption: fixative not stated). The reported paraffin IHC used heat-mediated EDTA retrieval at pH 8.0, but retrieval dependence and whether frozen sections or IF are easier are unreported (selected-SKU caption: EDTA pH 8.0; supplied target/application evidence). In bone marrow, endogenous myeloid peroxidase may produce DAB background, so score nuclear staining against the control slide (standard IHC practice; UniProt P33993: nucleus).
HPA tissue IHC evidence for MCM7
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Enhanced — High consistency between antibody staining and RNA expression data.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Appendix | Endocrine cells | High | Protein (IHC) | HPA → |
| Bone marrow | Hematopoietic cells | High | Protein (IHC) | HPA → |
| Bronchus | Basal cells | High | Protein (IHC) | HPA → |
| Colon | Endocrine cells | High | Protein (IHC) | HPA → |
| Duodenum | Endocrine cells | High | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | Adipocytes | Not detected | Protein (IHC) | HPA → |
| Caudate | Glial cells | Not detected | Protein (IHC) | HPA → |
| Cerebellum | Cells in granular layer | Not detected | Protein (IHC) | HPA → |
| Cerebral cortex | Endothelial cells | Not detected | Protein (IHC) | HPA → |
| Heart muscle | Cardiomyocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced MCM7 IHC Tips
Troubleshoot MCM7 staining in paraffin sections by checking retrieval, nuclear localisation, controls, and cell type before comparing scores (datasheet PB9261; UniProt P33993; HPA tissue IHC).
What should I adjust when nuclear MCM7 staining is weak?
Start with heat-mediated antigen retrieval in EDTA at pH 8.0 for paraffin sections (datasheet PB9261). The selected mouse testis image used that retrieval, followed by 10% goat serum and 1 μg/ml antibody overnight at 4°C (datasheet PB9261). If nuclear staining is weak, compare retrieval duration across matched sections while holding antibody concentration and DAB development constant; use a proliferative compartment as an internal reference (HPA: nuclear expression in highly proliferative cells; standard IHC practice). Check tissue integrity before intensifying heat, since excessive retrieval can damage morphology and complicate nuclear scoring (standard IHC practice).
Could fixation explain inconsistent MCM7 staining between paraffin blocks?
The selected caption identifies a paraffin section but gives no fixative, so MCM7-specific fixation sensitivity is unknown (datasheet PB9261). Record each block’s fixation and processing history, then compare matched sections using EDTA retrieval at pH 8.0 and 1 μg/ml antibody (datasheet PB9261; standard IHC practice). Assess nuclear signal in a proliferative compartment alongside nuclear morphology; loss of staining alone cannot establish that fixation damaged the epitope (HPA: nuclear expression in highly proliferative cells; standard IHC practice). If blocks differ, also check section age, retrieval consistency, and DAB development before assigning the difference to MCM7 biology (standard IHC practice).
How should I evaluate cytoplasmic or patchy MCM7 staining?
Prioritise nuclear staining when assessing paraffin-section IHC, because MCM7 is annotated in the nucleus and on chromosomes, with nuclear expression in highly proliferative cells (UniProt P33993 localisation; HPA tissue IHC). Its chromatin association changes as DNA replication progresses, so variation among nuclei can be biologically plausible without proving a specific cell-cycle phase (UniProt P33993 localisation; standard IHC interpretation). Cytosolic and basal-body localisation are additionally reported in subcellular imaging, but that evidence does not establish a cytoplasmic DAB scoring rule for this antibody (HPA subcellular; datasheet PB9261). Compare compartment, morphology, and omission-of-primary controls before accepting diffuse cytoplasmic colour as specific staining (standard IHC practice).
Can this IHC stain distinguish MCM7 isoforms or phosphorylation states?
MCM7 has 3 annotated isoforms and several modified residues, including phosphoserines at positions 121, 314, 365, and 500 (UniProt P33993 isoforms and modified residues). The supplied antibody caption does not identify an epitope, so its isoform coverage and sensitivity to those modifications remain undetermined (datasheet PB9261). Request epitope or immunogen documentation before interpreting unequal nuclear staining as an isoform-specific or phosphorylation-specific pattern (standard IHC interpretation). For comparisons, process adjacent sections together, keep EDTA retrieval at pH 8.0 and antibody at 1 μg/ml, and score the same cell population (datasheet PB9261; standard IHC practice).
How can companion IF help assess an MCM7 IHC pattern?
For companion IF, multiplex MCM7 with a proliferation marker such as Ki-67 and a nuclear counterstain, then compare marker-positive nuclei with the chromogenic pattern in corresponding cell populations (HPA: nuclear expression in highly proliferative cells; standard IF practice). Choose a far-red fluorophore where tissue autofluorescence obscures shorter wavelengths, and include single-colour and no-primary controls to separate bleed-through from signal (standard IF practice). Permeabilise sufficiently for a nuclear epitope: MCM7 is nuclear or chromosome-associated and has no transmembrane segment (UniProt P33993 localisation and topology; standard IF practice). Establish IF fixation and antibody settings independently, because the selected paraffin IHC caption supplies no IF fixation or staining conditions (datasheet PB9261).
What causes diffuse DAB colour or staining outside nuclei?
First compare the stained section with a no-primary control and inspect whether colour follows tissue edges, damaged areas, or the expected nuclei (standard IHC practice; HPA: nuclear expression in highly proliferative cells). The selected workflow uses a biotinylated secondary, streptavidin-biotin complex, and DAB, so check endogenous biotin and endogenous peroxidase controls when background persists (datasheet PB9261; standard IHC practice). Apply a peroxidase block and optimise washing and DAB development as general chromogenic IHC steps, rather than treating them as demonstrated MCM7-specific requirements (standard IHC practice). Keep the documented 10% goat-serum block and 1 μg/ml primary concentration as comparison points while changing one condition at a time (datasheet PB9261; standard IHC practice).
How should I score MCM7 across sections with different cellularity? ⚠ ANSWER MARKED FOR VERIFICATION
Define the cell population and score nuclear DAB staining, consistent with the reported nuclear pattern in proliferative cells (HPA tissue IHC; standard IHC practice). Report % positive nuclei or an H-score using intensity grades 0–3, with a possible H-score range of 0–300; set the positivity threshold before reviewing groups (standard IHC scoring practice). Normalise positive counts to the number of eligible nuclei in that cell population, or report positive nuclei per mm² of viable tissue when spatial density matters (standard IHC scoring practice). Exclude folds, edges, and necrotic regions consistently, and keep retrieval and DAB development comparable across sections (standard IHC practice; datasheet PB9261: EDTA pH 8.0 retrieval).
When is an apparent MCM7-positive cell likely to be an artefact?
A credible result places signal mainly in nuclei of the assessed cell population, consistent with MCM7’s nuclear and chromosome annotation and the tissue IHC profile (UniProt P33993 localisation; HPA tissue IHC). Check cell identity: hematopoietic cells in bone marrow and germinal-center cells in lymph nodes show high staining, whereas adipocytes are reported as undetected (HPA tissue IHC). Colour confined to section edges, necrotic areas, or a no-primary control supports a processing or detection artefact; endogenous peroxidase can also produce misleading DAB colour (standard IHC practice). Resolve doubtful cases using preserved morphology, matched controls, and the same EDTA pH 8.0 retrieval before interpreting differences biologically (standard IHC practice; datasheet PB9261).
Boster reagents
Best MCM7 / DNA replication licensing factor MCM7 IHC Antibodies
Anti-MCM7 IHC figures cover mouse testis and human laryngeal squamous cell carcinoma and bladder cancer (catalog IHC captions); IF figures cover human colon cancer, PC-3, and MCF-7 (catalog IF captions).
Real IHC data
Anti-MCM7 Antibody ®
Cat # PB9261
Real IHC data
Anti-MCM7 Antibody ® (monoclonal, 3H11)
Cat # M01649-3
Real IHC data
Anti-MCM7 Antibody ®
Cat # PA1792
PB9261 has paraffin-section IHC images in mouse testis, rat intestine, and human lung cancer (PB9261 IHC captions), plus IF images in U20S cells and human colon cancer sections (PB9261 IF captions). M01649-3 has paraffin-section IHC images in human laryngeal squamous cell carcinoma, colon adenocarcinoma, and chronic tonsillitis (M01649-3 IHC captions), plus IF in PC-3 cells (M01649-3 IF caption); PA1792 has paraffin-section IHC images in human bladder cancer, ovarian serous adenocarcinoma, and rectal adenocarcinoma (PA1792 IHC captions), plus IF in MCF-7 cells (PA1792 IF caption).
Which to pick: For human paraffin-section IHC, M01649-3 is a mouse monoclonal antibody, clone 3H11 (catalog: host and clone), pictured in laryngeal squamous cell carcinoma after EDTA retrieval (M01649-3 IHC caption); PA1792 is pictured in bladder cancer after EDTA retrieval (PA1792 IHC caption). For IF/ICC in PC-3 cells, M01649-3 has an IF image and lists both applications (M01649-3 IF caption; catalog: applications). For work across human, mouse, and rat, PB9261 lists all three species and IHC/IF/ICC applications (PB9261 catalog), with paraffin-section IHC images in each species (PB9261 IHC captions); the fixative is unreported in those captions (PB9261 IHC captions).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry P33993 (MCM7_HUMAN, DNA replication licensing factor MCM7).
- Human Protein Atlas. MCM7 tissue IHC expression (reliability: Enhanced).
- Human Protein Atlas. MCM7 subcellular location (ICC-IF): Mainly localized to the nucleoplasm. In addition localized to the cytosol and basal body..
- Human Protein Atlas. MCM7 antibody validation summary (3 antibodies).
- Minichromosome maintenance protein 7 as a potential prognostic factor for progression-free survival in high-grade serous carcinomas of the ovary. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2011 — PMC3964599.
- Minichromosome Maintenance Protein 7 is a potential therapeutic target in human cancer and a novel prognostic marker of non-small cell lung cancer. Molecular cancer 2011 — PMC3125391.
- Aberrant expression of NEK2 and its clinical significance in non-small cell lung cancer. Oncology letters 2014 — PMC4156209.
- MCM4 and MCM7, potential novel proliferation markers, significantly correlated with Ki-67, Bmi1, and cyclin E expression in esophageal adenocarcinoma, squamous cell carcinoma, and precancerous lesions. Human pathology 2016 — PMC5250507.
- PubMed PMID:8626784 — UniProt-cited evidence.
- PubMed PMID:14702039 — UniProt-cited evidence.
- PubMed PMID:12690205 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for MCM7 (DNA replication licensing factor MCM7). Expected localisation Nuclear staining observed in proliferative cells (HPA tissue IHC). Antigen retrieval: EDTA pH 8.0 HIER, heat-mediated (datasheet PB9261). Validated IHC antibody: PB9261 at 0.5-1μg/ml (the product's own IHC validation image is shown). Controls (HPA): positive Appendix (Endocrine cells); negative Adipose tissue. IHC workflow; specimen preparation unspecified. Protocol steps: 1. Sample: Paraffin-embedded Mouse Testis tissue; fixative not specified (datasheet PB9261); 2. Fixation: Image fixative and duration unreported (datasheet PB9261); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: EDTA pH 8.0 (datasheet PB9261); 20 min, 95–100 °C (standard); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% goat serum (datasheet PB9261); 8. Primary antibody: Rabbit anti-MCM7, 0.5-1μg/ml (datasheet PB9261); 9. Primary incubation: Overnight at 4 °C (datasheet PB9261). Watch for: Fixation: Keep fixation consistent across sections (standard IHC practice; not target-specific); Caveat: Proliferation differences can change the stained-cell fraction (HPA tissue IHC); Isoform / epitope: Three isoforms; epitope coverage is unspecified (UniProt); Regulation: Enriched in highly proliferative cells (HPA tissue IHC). Evidence: UniProt P33993, HPA tissue IHC · Enhanced, HPA ICC-IF · Mainly localized to the nucleoplasm. In addition localized to the…, Datasheet PB9261, PMC3964599, PMC3125391. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/MCM7/immunohistochemistry-ihc-guide.