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MCM7 / DNA replication licensing factor MCM7 · IHC design guide
Design Immunohistochemistry for MCM7
MCM7 is a nuclear DNA replication licensing factor (UniProt), and tissue IHC shows nuclear staining in highly proliferative cells (HPA tissue IHC). Use this paraffin-section guide to plan controls and score nuclear staining, with bone marrow hematopoietic cells as a high-staining reference (HPA tissue IHC).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
MCM7 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Nuclear staining in highly proliferative cells (HPA tissue IHC) | |
| Staining pattern | Proliferative cells show nuclear staining (HPA tissue IHC) | |
| Antigen retrieval | EDTA pH 8.0 HIER, heat-mediated (datasheet PB9261) | |
| Positive control | Appendix+4 more · see all | |
| Negative control | Adipose tissue+4 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image PB9261) | |
| Caveat | Adipocytes may be unstained despite strong marrow staining (HPA tissue IHC) | |
| Regulation | Higher in highly proliferative cells (HPA tissue IHC) | |
| Isoform / epitope | Three isoforms; epitope coverage needs validation (UniProt) |
Section 1
Recommended MCM7 IHC & IF Protocols
The catalog antibody’s IHC-P protocol uses EDTA pH 8.0 retrieval (datasheet: PB9261). The published IHC protocols below provide tissue-specific methods (PMC3964599; PMC3125391; PMC4156209).
| Sample | Paraffin-embedded Mouse Testis tissue; fixative not specified (datasheet PB9261) |
| Fixation | Image fixative and duration unreported (datasheet PB9261); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: EDTA pH 8.0 (datasheet PB9261); 20 min, 95–100 °C (standard) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% goat serum (datasheet PB9261) |
| Primary antibody | Rabbit anti-MCM7, 0.5-1μg/ml (datasheet PB9261) |
| Primary incubation | Overnight at 4 °C (datasheet PB9261) |
| Detection | Streptavidin-biotin complex (SABC), DAB chromogen (datasheet PB9261) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | MCM7-positive staining in endocrine cells of appendix (HPA tissue IHC: High). HPA tissue profile: Nuclear expression in highly proliferative cells. No signal in the no-primary control. |
💡Decision noteStart with heat-mediated EDTA pH 8.0 retrieval for the catalog antibody (datasheet: PB9261); citrate pH 6.0 is a published alternative for NSCLC sections (PMC4156209).
Section 2
What Is the Expected MCM7 Staining Pattern?
MCM7 should stain nuclei, particularly in highly proliferative cells (HPA: nuclear expression in highly proliferative cells). Nuclear and chromosome localisation fits its role in DNA replication (UniProt P33993: localisation and function). HPA rates its tissue IHC pattern Enhanced, reflecting high consistency between staining and RNA expression (HPA: tissue IHC reliability). MCM7 has no transmembrane segment (UniProt P33993: topology).
| Strong, discrete nuclear staining in bone marrow hematopoietic cells or lymph node germinal center cells (HPA: High in both). | This fits the reported IHC pattern; score the relevant cells and their nuclei, rather than treating every nucleus in the section as an expected positive (HPA: nuclear expression in highly proliferative cells; general IHC practice). |
| Predominantly cytoplasmic or membrane staining, with little nuclear signal, in an IHC section. | Question the result because the expected tissue IHC pattern is nuclear (HPA: tissue IHC profile). UniProt places MCM7 in the nucleus and on chromosomes and reports no transmembrane segment (UniProt P33993: localisation and topology). |
| Strong staining in adipocytes or heart muscle cardiomyocytes (HPA: Not detected in those cells). | Investigate antibody cross-reactivity or endogenous detection activity before calling those cells MCM7 positive (general IHC practice). The HPA finding is cell specific; it does not mean every cell in either tissue must be negative (HPA: tissue IHC). |
| Diffuse colour over nuclei, cytoplasm and empty areas, obscuring cell boundaries. | Treat this as background until a no-primary control and a known-positive section clarify whether detection chemistry or nonspecific binding contributes (general IHC practice). Diffuse staining alone does not reproduce HPA's nuclear pattern (HPA: tissue IHC profile). |
| No nuclear staining in bone marrow hematopoietic cells despite intact tissue (HPA: High). | First check the staining run with a known-positive control and confirm that the expected cells are present (general IHC practice). A blank result may reflect assay failure; it does not by itself revise the HPA observation (HPA: tissue IHC). |
💡Expected MCM7 appearanceCall a result positive when relevant proliferative cells show clear nuclear staining, with High signal possible in HPA-listed positive cell types; widespread membrane staining or diffuse colour without discernible nuclei is suspect (HPA: tissue IHC profile and levels; UniProt P33993: topology; general IHC practice).
| Cell population chosen for evaluation | HPA reports High staining in bronchial basal cells, esophageal squamous epithelial cells and lymph node germinal center cells, but Not detected in adipocytes and cardiomyocytes. Compare the named cell populations, not whole-tissue averages (HPA: tissue IHC; general IHC practice). |
| Cell-cycle context | MCM7 participates in replication initiation and elongation, and its chromatin association changes as replication progresses (UniProt P33993: function and localisation). Interpret variable nuclear staining alongside the HPA pattern in highly proliferative cells; nuclear intensity alone does not assign a precise cell-cycle phase (HPA: tissue IHC profile; general IHC practice). |
| Antibody validation | HPA lists 3 antibodies with Enhanced IHC validation: HPA003898, CAB002163 and CAB016312 (HPA: antibody validation). This supports the reported pattern but does not establish that an unlisted catalog antibody has identical specificity (general IHC practice). |
| IF/ICC Q&A: must every signal be nuclear? | No. HPA approves nucleoplasm as the main ICC-IF location and also reports cytosol and basal body (HPA: ICC-IF localisation). Those additional ICC-IF observations do not make predominantly cytoplasmic staining the expected paraffin IHC result (HPA: tissue IHC profile). |
| Epitope and protein forms | UniProt lists 3 isoforms and several modified residues, but the supplied record does not map an antibody epitope to them (UniProt P33993: isoforms and modified residues; HPA: antibody list). Do not infer isoform selectivity, retrieval requirements or modification-dependent staining from these entries. |
| Processing and topology | UniProt reports no signal peptide, no propeptide and no transmembrane segment (UniProt P33993: processing and topology). A membrane-dominant IHC pattern therefore needs independent verification; these annotations alone do not predict fixation sensitivity or a particular antigen-retrieval condition. |
| Situation | Likely cause | Next action |
|---|---|---|
| Known-positive bone marrow section lacks nuclear signal (HPA: High in hematopoietic cells). | The run may have failed, or the examined field may lack the named positive cells (general IHC practice). | Confirm cell identity and inspect the run control, then check the antibody and detection steps against their instructions (general IHC practice). |
| Strong colour persists across cells and empty areas. | Nonspecific binding or detection background may obscure a true nuclear pattern (general IHC practice; HPA: tissue IHC profile). | Compare a no-primary control; review blocking, washes and detection exposure using general IHC practice. Do not score diffuse colour as nuclear positivity. |
| Signal is mainly at membranes or in cytoplasm on paraffin IHC. | The compartment disagrees with HPA tissue IHC and UniProt localisation (HPA: nuclear profile; UniProt P33993: nucleus and chromosome). | Check morphology and a known-positive nuclear control; investigate nonspecific staining before interpreting the signal as MCM7 (general IHC practice). |
| Adipocytes or cardiomyocytes stain strongly (HPA: Not detected in those cells). | Cross-reactivity or endogenous detection activity is possible; the HPA comparison applies to the named cell types (general IHC practice; HPA: tissue IHC). | Review cell identity and no-primary controls, then compare a listed positive cell population in the same run (HPA: tissue IHC; general IHC practice). |
| Nuclear signal is weak in a section expected to contain positive cells. | The relevant cell population may be sparse, or assay conditions may reduce visibility; target-specific fixation sensitivity is unreported here (HPA: cell-specific levels; general IHC practice). | Locate the named cells, check run controls and review the antibody's IHC-P instructions before adjusting routine assay conditions (general IHC practice). |
| IF/ICC shows a small extranuclear signal alongside nucleoplasmic staining. | HPA also approves cytosol and basal body localisation in ICC-IF (HPA: ICC-IF localisation). | Interpret it with the ICC-IF evidence and suitable imaging controls; use the separate IF/ICC guide for assay setup (HPA: ICC-IF localisation; general IF practice). |
Experiment evidence
IHC & IF images showing MCM7 staining
PB9261 · IHCIHC analysis of MCM7 using anti-MCM7 antibody (PB9261). MCM7 was detected in a paraffin-embedded section of Mouse Testis tissue. Heat mediat
PB9261 · IHCIHC analysis of MCM7 using anti-MCM7 antibody (PB9261). MCM7 was detected in a paraffin-embedded section of Mouse Testis tissue. Heat mediat
PB9261 · IFIF analysis of MCM7 using anti-MCM7 antibody (PB9261). MCM7 was detected in immunocytochemical section of U20S cells. Enzyme antigen retriev
M01649-3 · IHCIHC analysis of MCM7 using anti-MCM7 antibody (M01649-3). MCM7 was detected in a paraffin-embedded section of human laryngeal squamous cell
M01649-3 · IHCIHC analysis of MCM7 using anti-MCM7 antibody (M01649-3). MCM7 was detected in a paraffin-embedded section of human adenocarcinoma of the co
M01649-3 · IFIF analysis of MCM7 using anti-MCM7 antibody (M01649-3). MCM7 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen re
PA1792 · IHCIHC analysis of MCM7 using anti-MCM7 antibody (PA1792). MCM7 was detected in a paraffin-embedded section of human bladder cancer tissue. Hea
PA1792 · IHCIHC analysis of MCM7 using anti-MCM7 antibody (PA1792). MCM7 was detected in a paraffin-embedded section of human ovarian serous adenocarcin
PA1792 · IFIF analysis of MCM7 using anti-MCM7 antibody (PA1792) and anti-Tubulin Alpha antibody (M03989-3). MCM7 was detected in immunocytochemical se
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000166508-MCM7/tissue/cerebral+cortex#imid_4208940
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000166508-MCM7/tissue/colon#imid_4208874
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000166508-MCM7/tissue/liver#imid_4208883
HPA · KidneyHuman Protein Atlas IHC, Kidney. Source: https://www.proteinatlas.org/ENSG00000166508-MCM7/tissue/kidney#imid_4208884
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody CAB016312; green = MCM7, blue = nucleus, red = microtubules.
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody CAB016312; green = MCM7, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for MCM7 IHC & IF
🧪Run bronchus first and look for nuclear staining in basal cells (HPA: High in bronchus basal cells; UniProt P33993: nucleus). Use heart muscle as the negative tissue and assess cardiomyocytes, where MCM7 was not detected (HPA: Not detected in heart muscle cardiomyocytes); on the bronchus slide, use adjacent cells that show no nuclear signal as internal negatives without assuming every non-basal cell is MCM7-negative.
Positive control tissue: Appendix (Endocrine cells, HPA High)
Negative control tissue: Adipose tissue (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show MCM7 in A-431, U-251MG, U2OS, MCF-7, ASC52telo, RPTEC/TERT1, hTERT-RPE1 (serum starved), with annotated localisation: Nucleoplasm (approved) (HPA subcellular).
Technical controls: Include a no-primary, secondary-only control; a concentration-matched rabbit IgG control appropriate to the catalog antibody’s clonality (catalog antibody caption: rabbit anti-MCM7); and MCM7 knockout tissue or cells as a biological negative. For chromogenic detection in bronchus, quench endogenous peroxidase and check for endogenous biotin when using the caption’s biotinylated secondary and streptavidin–biotin detection (PB9261 tissue-IHC caption: biotinylated secondary, SABC and DAB).
⚠️Feasibility: A target-specific fixation window or fixation effect is unreported, and the selected caption does not state a fixative (PB9261 tissue-IHC caption: fixative not stated). The paraffin-section example uses heat retrieval in EDTA at pH 8.0, but whether retrieval is required is unreported (PB9261 tissue-IHC caption: EDTA heat retrieval). Neither frozen sections nor IF are established as easier by the supplied evidence; for IF, interpret nuclear signal alongside possible cytosolic and basal-body signal, and check bronchial autofluorescence (HPA subcellular: approved nucleoplasm, cytosol and basal body locations; standard IF practice).
HPA tissue IHC evidence for MCM7
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Enhanced — High consistency between antibody staining and RNA expression data.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Appendix | Endocrine cells | High | Protein (IHC) | HPA → |
| Bone marrow | Hematopoietic cells | High | Protein (IHC) | HPA → |
| Bronchus | Basal cells | High | Protein (IHC) | HPA → |
| Colon | Endocrine cells | High | Protein (IHC) | HPA → |
| Duodenum | Endocrine cells | High | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | Adipocytes | Not detected | Protein (IHC) | HPA → |
| Caudate | Glial cells | Not detected | Protein (IHC) | HPA → |
| Cerebellum | Cells in granular layer | Not detected | Protein (IHC) | HPA → |
| Cerebral cortex | Endothelial cells | Not detected | Protein (IHC) | HPA → |
| Heart muscle | Cardiomyocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced MCM7 IHC Tips
Troubleshoot MCM7 staining by checking retrieval, nuclear localisation, cell type and detection controls before comparing chromogenic IHC scores.
How should I retrieve MCM7 in paraffin sections when nuclear staining is weak?
Start with heat mediated retrieval in EDTA at pH 8.0 for paraffin sections (datasheet PB9261). The catalog antibody produced MCM7 staining after this retrieval in a mouse testis paraffin section, with 1 μg/ml primary antibody incubated overnight at 4°C (datasheet PB9261). If nuclei remain weak, check that sections stayed covered by retrieval buffer and compare a fresh retrieval run before increasing primary antibody concentration (standard IHC practice). Assess signal in cells expected to proliferate and preserve nuclear detail, since MCM7 is chiefly nuclear and associated with chromatin before DNA replication progresses (HPA tissue IHC; UniProt P33993 localisation).
Could fixation explain weak MCM7 staining in my paraffin sections?
Target specific MCM7 sensitivity to fixation is unknown from the supplied evidence; the catalog image identifies a paraffin section but does not state its fixative (datasheet PB9261). Record the fixative, fixation duration and tissue thickness for each specimen, then compare sections processed under matched conditions (standard IHC practice). If nuclear signal varies across a batch, check tissue preservation and retrieval consistency before attributing the difference to MCM7 biology (standard IHC practice). Use the reported EDTA retrieval at pH 8.0 as the starting condition, and assess nuclear staining with an appropriate positive control (datasheet PB9261; HPA tissue IHC).
What should I make of cytoplasmic MCM7 staining in chromogenic IHC?
Prioritise nuclear staining when scoring tissue sections: MCM7 is reported in the nucleus and on chromosomes, and tissue IHC shows nuclear expression in highly proliferative cells (UniProt P33993 localisation; HPA tissue IHC). HPA cell imaging also reports cytosol and basal body localisation in addition to a mainly nucleoplasmic pattern, so cytoplasmic signal alone is not conclusive evidence of an artefact (HPA subcellular). Compare nuclear and cytoplasmic staining separately, using matched exposure and chromogen development across sections (standard IHC practice). If diffuse cytoplasmic colour obscures nuclei, inspect the no primary control and reduce background before assigning cell positivity (standard IHC practice).
Can isoforms or epitope changes explain discordant MCM7 IHC results?
MCM7 has 3 annotated isoforms and several modified residues, including phosphoserines at positions 121, 314, 365 and 500 (UniProt P33993 isoforms; UniProt P33993 modified residues). Its MCM C terminal AAA(+) ATPase domain spans residues 316–647, but the supplied catalog caption does not identify the antibody epitope (UniProt P33993 domains; datasheet PB9261). Therefore, an isoform or modification effect on this antibody's IHC signal remains unestablished (UniProt P33993 isoforms; datasheet PB9261). Compare matched sections and controls before invoking epitope biology; request mapped epitope information if isoform specific interpretation is essential (standard IHC practice).
How can IF help assess MCM7 localisation alongside chromogenic IHC?
For a separate IF experiment, multiplex MCM7 with a marker that identifies the expected cell population, and evaluate nuclear overlap at the single cell level (HPA tissue IHC; standard IF practice). Choose a fluorophore channel with low background from the specimen's autofluorescence, and check each channel with single stain controls (standard IF practice). MCM7 has no transmembrane segment and is mainly nucleoplasmic, so use permeabilisation suitable for access to an intracellular nuclear epitope, then verify that nuclear morphology remains intact (UniProt P33993 topology; HPA subcellular; standard IF practice). Keep IF localisation observations distinct from the catalog antibody's paraffin section IHC result (datasheet PB9261).
How do I reduce diffuse or nonspecific DAB staining in MCM7 IHC?
First inspect a no primary control and shorten chromogen development if brown colour appears across the section (standard IHC practice). The catalog image used 10% goat serum blocking, a biotinylated secondary antibody, streptavidin biotin detection and DAB; those steps identify plausible points for nonspecific or endogenous background checks (datasheet PB9261; standard IHC practice). Apply a peroxidase block appropriate to the tissue, and include a detection only control when investigating endogenous enzyme or biotin related colour (standard IHC practice). Judge improvement by clearer nuclear contrast in expected proliferating cells, while retaining comparable counterstaining and section handling (HPA tissue IHC; standard IHC practice).
How should I score MCM7 staining across tissue sections? ⚠ ANSWER MARKED FOR VERIFICATION
Define the cell compartment and region before scoring, then report the percentage of positive nuclei or a nuclear H-score using consistent intensity categories (HPA tissue IHC; standard IHC practice). For spatial comparisons, positive nuclei per mm² can supplement the cell based score if the sampled area is recorded (standard IHC practice). Normalise positive counts to the number of evaluable nuclei in the same annotated cell population, and document excluded damaged or necrotic areas (standard IHC practice). Keep retrieval, antibody concentration and DAB development consistent; the catalog caption specifies EDTA pH 8.0 and 1 μg/ml antibody for its image (datasheet PB9261).
How can I distinguish genuine MCM7 positivity from staining artefacts?
A credible tissue pattern places signal in nuclei of an appropriate cell population, consistent with MCM7's nuclear localisation and HPA's staining of highly proliferative cells (UniProt P33993 localisation; HPA tissue IHC). High staining in bone marrow hematopoietic cells or lymph node germinal center cells can serve as a pattern check, while the supplied HPA profile reports no detection in adipocytes (HPA tissue IHC). Be cautious with isolated cytoplasmic colour, section edge staining or necrotic regions, and compare these areas with intact internal tissue and a no primary control (standard IHC practice). If DAB appears in the detection control, investigate endogenous peroxidase or detection reagent background before scoring nuclei (standard IHC practice).
Boster reagents
Best MCM7 / DNA replication licensing factor MCM7 IHC Antibodies
Anti-MCM7 antibodies have IHC images from paraffin sections of human, mouse, and rat tissues, plus IF images from human cells and colon sections (catalog image captions).
Real IHC data
Anti-MCM7 Antibody ®
Cat # PB9261
Real IHC data
Anti-MCM7 Antibody ® (monoclonal, 3H11)
Cat # M01649-3
Real IHC data
Anti-MCM7 Antibody ®
Cat # PA1792
PB9261 has IHC images from mouse testis, rat intestine, and human lung cancer; M01649-3 has IHC images from human laryngeal squamous cell carcinoma, colon adenocarcinoma, and chronic tonsillitis (catalog IHC captions). PA1792 has IHC images from human bladder cancer, ovarian serous adenocarcinoma, and rectal adenocarcinoma (catalog IHC captions).
Which to pick: For human tissue IHC, PA1792 has a paraffin-section image using EDTA retrieval at pH 8.0 and 2 μg/ml primary antibody (PA1792 IHC caption). For human IF/ICC, the mouse monoclonal M01649-3 has an IF image in PC-3 cells and lists both applications (M01649-3 catalog and IF caption). For IHC across human, mouse, and rat samples, PB9261 lists all three species and has paraffin-section images from each; the captions do not report the fixative (PB9261 catalog and IHC captions).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry P33993 (MCM7_HUMAN, DNA replication licensing factor MCM7).
- Human Protein Atlas. MCM7 tissue IHC expression (reliability: Enhanced).
- Human Protein Atlas. MCM7 subcellular location (ICC-IF): Mainly localized to the nucleoplasm. In addition localized to the cytosol and basal body..
- Human Protein Atlas. MCM7 antibody validation summary (3 antibodies).
- Minichromosome maintenance protein 7 as a potential prognostic factor for progression-free survival in high-grade serous carcinomas of the ovary. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2011 — PMC3964599.
- Minichromosome Maintenance Protein 7 is a potential therapeutic target in human cancer and a novel prognostic marker of non-small cell lung cancer. Molecular cancer 2011 — PMC3125391.
- Aberrant expression of NEK2 and its clinical significance in non-small cell lung cancer. Oncology letters 2014 — PMC4156209.
- MCM4 and MCM7, potential novel proliferation markers, significantly correlated with Ki-67, Bmi1, and cyclin E expression in esophageal adenocarcinoma, squamous cell carcinoma, and precancerous lesions. Human pathology 2016 — PMC5250507.
- PubMed PMID:8626784 — UniProt-cited evidence.
- PubMed PMID:14702039 — UniProt-cited evidence.
- PubMed PMID:12690205 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for MCM7 (DNA replication licensing factor MCM7). Expected localisation Nuclear staining in highly proliferative cells (HPA tissue IHC). Antigen retrieval: EDTA pH 8.0 HIER, heat-mediated (datasheet PB9261). Validated IHC antibody: PB9261 at 0.5-1μg/ml (the product's own IHC validation image is shown). Controls (HPA): positive Appendix (Endocrine cells); negative Adipose tissue. IHC workflow; specimen preparation unspecified. Protocol steps: 1. Sample: Paraffin-embedded Mouse Testis tissue; fixative not specified (datasheet PB9261); 2. Fixation: Image fixative and duration unreported (datasheet PB9261); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: EDTA pH 8.0 (datasheet PB9261); 20 min, 95–100 °C (standard); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% goat serum (datasheet PB9261); 8. Primary antibody: Rabbit anti-MCM7, 0.5-1μg/ml (datasheet PB9261); 9. Primary incubation: Overnight at 4 °C (datasheet PB9261). Watch for: Fixation: Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image PB9261); Caveat: Adipocytes may be unstained despite strong marrow staining (HPA tissue IHC); Isoform / epitope: Three isoforms; epitope coverage needs validation (UniProt); Regulation: Higher in highly proliferative cells (HPA tissue IHC). Evidence: UniProt P33993, HPA tissue IHC · Enhanced, HPA ICC-IF · Mainly localized to the nucleoplasm. In addition localized to the…, Datasheet PB9261, PMC3964599, PMC3125391. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/MCM7/immunohistochemistry-ihc-guide.