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- Table of Contents
MEIG1 / Meiosis expressed gene 1 protein homolog · Western blot design guide
Design a Western Blot for MEIG1
Source-linked MEIG1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-MEIG1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled October 2026 · For research use; verify linked source records and product datasheet before use
MEIG1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~10.8 kDa | |
| Observed band | 68 kDa | |
| Gel | 15% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | Blocking peptide control | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked MEIG1 Western Blot Protocol Options
The A16264 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | mouse lung tissue lysate (catalog A16264) |
| Gel % | 15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.2 µm PVDF (standard starting point) |
| Blocking | Western blot analysis of MEIG in mouse lung tissue lysate with MEIG antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide (catalog A16264) |
| Primary antibody | A16264 · 1 μg/mL (catalog A16264) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected MEIG1 Western Blot Band Size?
MEIG1 is predicted at 10.8 kDa, but the supplied empirical band is 68 kDa; the cause of the difference is not established.
| Band near 10.8 kDa | Near the UniProt predicted mass; confirm MEIG1 identity. |
| Band at 68 kDa | Matches the supplied empirical band; MEIG1 identity requires controls. |
| Bands near 10.8 and 68 kDa | Both the predicted position and empirical band appear; their relationship is unestablished. |
| No band near 10.8 kDa | The predicted mass does not establish where detectable MEIG1 migrates. |
💡Expected MEIG1 appearanceUniProt predicts 10.8 kDa, while antibody QC reports a 68 kDa band; the cause of the difference is unestablished, so confirm identity with peptide competition and genetic controls.
| UniProt predicted molecular weight | Places the sequence-based prediction near 10.8 kDa. |
| Predicted full-length mass | Provides a 10.8 kDa reference for the intact sequence. |
| Predicted unmodified mass | Provides a 10.8 kDa reference without an annotated modification effect. |
| Predicted mass versus empirical band | The 10.8 kDa prediction does not explain the observed 68 kDa band. |
| Situation | Likely cause | Next action |
|---|---|---|
| Band higher than expected | The observed 68 kDa band differs from the 10.8 kDa prediction for an unknown reason. | Test peptide competition and MEIG1 depletion to establish identity. |
| Band lower than expected | No annotated cleavage explains a smaller band. | Check sample integrity and test whether MEIG1 depletion removes the band. |
| Multiple bands | The supplied features do not establish distinct MEIG1 products. | Compare each band using peptide competition and MEIG1 depletion. |
| Broad smear instead of sharp band | No annotated modification establishes a MEIG1 smear. | Check sample quality, loading, and antibody specificity. |
| Weak or no signal | MEIG1 abundance or assay sensitivity may be insufficient in the tested sample. | Verify loading and transfer, then test a suitable positive-control lysate. |
Experiment evidence
Western blot images showing MEIG1 band
A16264 · MEIG1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for MEIG1 Western blot
🧪For positive controls for MEIG1 in Western blot, you can use a validated MEIG1-positive sample; the supplied HPA data identify no tissue or cell line.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA data identify no positive or negative tissue, so suitable controls require independent validation.
HPA tissue expression evidence for MEIG1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced MEIG1 Western Blot Tips
Deeper troubleshooting and optimisation questions for MEIG1, answered from its protein features.
How should MEIG1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could a MEIG1 isoform account for the 68 kDa band?
Isoforms · Only one isoform is listed, with an 88-residue canonical sequence. The supplied features provide no alternative sequence that explains a 68 kDa band.
Could annotated modifications explain a MEIG1 band shift?
PTM · No modified residues or glycosylation sites are listed. The supplied features therefore provide no specific modification to test as the cause of a shift. Lack of annotation does not establish that modification cannot occur.
Does this guide establish induction of MEIG1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for MEIG1 Western blot?
Transfer · Plan transfer around the predicted 10.8 kDa protein: use conditions and a membrane that retain small proteins, then check that MEIG1 has not passed through. A 68 kDa observed band does not establish the size of the MEIG1 species being transferred.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A16264 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
Which MEIG1 band should be used for quantitation?
Quantitation · Quantify a band only after establishing its identity and using the same band consistently across samples. The predicted mass is 10.8 kDa, whereas the supplied observed band is 68 kDa; the supplied features do not establish that the 68 kDa signal represents MEIG1.
Why might MEIG1 appear at 68 kDa instead of 10.8 kDa?
Interpretation · The supplied observed band is 68 kDa, while the predicted mass is 10.8 kDa. These features do not explain the difference. Verify the 68 kDa band’s identity before assigning it to MEIG1.
MEIG1 is reported to interact with PACRG and MORN3. Those interactions alone do not show that a complex survives Western blot preparation or migrates at 68 kDa. Confirm band identity before attributing it to a partner complex.
Compare them with the predicted 10.8 kDa mass and verify their identity. The listed features show one isoform and no annotated modifications or glycosylation sites, so they do not support assigning extra bands to specific isoforms or modifications.
MEIG1 is reported to interact with PACRG and MORN3. Those interactions alone do not show that a complex survives Western blot preparation or migrates at 68 kDa. Confirm band identity before attributing it to a partner complex.
Compare them with the predicted 10.8 kDa mass and verify their identity. The listed features show one isoform and no annotated modifications or glycosylation sites, so they do not support assigning extra bands to specific isoforms or modifications.
Boster reagents
MEIG1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-MEIG1 Antibody
Cat # A16264
The catalog reports one anti-MEIG1 antibody, A16264, for Western blotting. Its reported reactivity is human, mouse and rat. The supplied image shows mouse lung tissue lysate tested with and without blocking peptide; it does not demonstrate Western blot performance in every listed species.
Which to pick: A16264 is the only listed option. Its Western blot image uses mouse lung tissue lysate at 1 μg/mL, with and without blocking peptide. For human or rat samples, reactivity is listed, but no sample-specific Western blot image is supplied.
Source: BosterBio MEIG1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q5JSS6.
- Human Protein Atlas. MEIG1 tissue expression.
- PMC4120260 — target-verified WB comparison
- PMC4698733 — target-verified WB comparison