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- Table of Contents
MEIS2 / Homeobox protein Meis2 · Western blot design guide
Design a Western Blot for MEIS2
Real validated MEIS2 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-MEIS2 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
MEIS2 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~51.8 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Adrenal gland (IHC candidate; verify WB) +4 more | |
| Negative control | Cerebellum (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 8 isoform(s) |
Section 1
Real Curated MEIS2 Western Blot Protocols
The M04264-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | HepG2 cell lysate (catalog M04264-1) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | M04264-1; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected MEIS2 Western Blot Band Size?
MEIS2 has a predicted monomer mass of 51.8 kDa; isoforms and homodimerization could affect bands, but their migration has not been demonstrated here.
| Band near 51.8 kDa | Consistent with the predicted MEIS2 monomer; confirm identity with controls |
| Several bands at different positions | Could reflect MEIS2 isoforms 1 through 8; their migration is not supplied |
| Band near 104 kDa | Could reflect an SDS-stable MEIS2 homodimer; homodimer formation alone does not establish this band |
| Little or no band in a cytoplasmic fraction | Consistent with MEIS2 nuclear localization; check a nuclear fraction |
💡Expected MEIS2 appearanceMEIS2 has a predicted monomer mass of 51.8 kDa, but no empirical band size is supplied; verify any candidate band with antibody specificity and appropriate fractionation controls.
| Predicted monomer mass | Sets a 51.8 kDa sequence-based reference |
| Monomeric MEIS2 | May migrate near the predicted monomer size; actual migration is unreported |
| MEIS2 homodimer | Could yield a band near twice the monomer size if it survives sample preparation |
| Splice isoforms 1, 2, 3, 4, 5, 6, 7 and 8 | May differ in apparent size; individual masses and resolvable differences are not supplied |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Nuclear MEIS2 may be insufficiently recovered or diluted in whole-cell lysate | Check a nuclear extract and verify fraction recovery |
| Band higher than expected | An SDS-stable MEIS2 homodimer is possible | Compare reducing and nonreducing samples and confirm band identity |
| Band lower than expected | A MEIS2 splice isoform is possible, but its mass is unknown | Check antibody epitope coverage and compare with an independent MEIS2 antibody |
| Multiple bands | MEIS2 has eight named splice isoforms, though distinct bands are unproven | Compare isoform expression and use MEIS2 depletion to identify specific bands |
| Weak or no signal | Nuclear localization may reduce MEIS2 signal in an unfractionated sample | Enrich the nuclear fraction and check loading and antibody performance |
| Fragments below expected size | Sample degradation or nonspecific binding is possible; no MEIS2 cleavage feature is supplied | Check sample handling and confirm bands with MEIS2 depletion |
Experiment evidence
Western blot images showing MEIS2 band
M04264-1 · MEIS2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for MEIS2 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for MEIS2 in Western blot, you can use adrenal gland tissue, which has high HPA expression.
Positive control: Adrenal gland (IHC candidate; verify WB)
Negative control: Cerebellum (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: MEIS2 is nuclear and perinuclear, so ensure the lysate retains nuclear proteins.
HPA tissue expression evidence for MEIS2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | glandular cells | High | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | High | Protein (IHC) | HPA → |
| Caudate | neuronal cells | High | Protein (IHC) | HPA → |
| Cervix | glandular cells | High | Protein (IHC) | HPA → |
| Endometrium | cells in endometrial stroma | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cerebellum | cells in molecular layer | Not detected | Protein (IHC) | HPA → |
| Heart muscle | cardiomyocytes | Not detected | Protein (IHC) | HPA → |
| Liver | cholangiocytes | Not detected | Protein (IHC) | HPA → |
| Parathyroid gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Rectum | glandular cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced MEIS2 Western Blot Tips
Deeper troubleshooting and optimisation questions for MEIS2, answered from its protein features.
How should MEIS2 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Which MEIS2 isoform has the largest annotated deletion?
Isoforms · Isoform 5 lacks canonical residues 303–477 and replaces HP at 301–302 with VY. It could produce a substantially smaller product than canonical MEIS2. Check whether the antibody epitope is retained before assigning a smaller band to this isoform.
Isoforms 2, 3, 6, 7, and 8 lack canonical residues 402–477. Isoforms 3, 6, and 7 also lack 346–352; isoform 6 replaces residues 1–89 with M, while isoforms 7 and 8 lack 1–13. These differences suggest possible size differences, but do not establish where bands will migrate.
Isoforms 2, 3, 6, 7, and 8 lack canonical residues 402–477. Isoforms 3, 6, and 7 also lack 346–352; isoform 6 replaces residues 1–89 with M, while isoforms 7 and 8 lack 1–13. These differences suggest possible size differences, but do not establish where bands will migrate.
Can an annotated modification explain an unexpected MEIS2 band?
PTM · The supplied UniProt features list no modified residues or glycosylation sites. They therefore provide no basis for assigning an unexpected band to a specific modification. Likewise, the annotated isoform differences alone do not establish the identity of an observed band.
Does this guide establish induction of MEIS2?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for MEIS2?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M04264-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should MEIS2 bands be quantified across samples?
Quantitation · Use the same defined band or set of bands across samples, and record which isoforms the antibody epitope can detect. Eight annotated isoforms mean a single band may not represent total MEIS2, while summing bands may combine different isoforms. State which signal is being compared.
Why might MEIS2 migrate away from its predicted 51.8 kDa?
Interpretation · 51.8 kDa is the predicted mass for the 477-residue canonical sequence. MEIS2 has eight annotated isoforms with sequence differences that may change calculated mass. No observed band size is supplied, so these features alone cannot explain a particular migration difference.
Map the epitope against the annotated alternative sequences. An epitope within canonical residues 402–477 is absent from isoforms 2, 3, 5, 6, 7, and 8. An epitope within 303–477 is absent from isoform 5. This determines which isoforms an antibody could detect.
UniProt lists MEIS2 in the nucleus and in the cytoplasmic perinuclear region. A band in either fraction can therefore be consistent with its annotated localization. Compare fractions using the same antibody and account for its isoform coverage before interpreting differences in signal.
Map the epitope against the annotated alternative sequences. An epitope within canonical residues 402–477 is absent from isoforms 2, 3, 5, 6, 7, and 8. An epitope within 303–477 is absent from isoform 5. This determines which isoforms an antibody could detect.
UniProt lists MEIS2 in the nucleus and in the cytoplasmic perinuclear region. A band in either fraction can therefore be consistent with its annotated localization. Compare fractions using the same antibody and account for its isoform coverage before interpreting differences in signal.
Boster reagents
MEIS2 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-MEIS2 Rabbit Monoclonal Antibody
Cat # M04264-1
The catalog reports one anti-MEIS2 rabbit monoclonal antibody, M04264-1, with reported human, mouse, and rat reactivity. Its Western blot image uses HepG2 cell lysate; the supplied evidence does not show Western blots in mouse or rat samples.
Which to pick: M04264-1 is the only listed option and has a Western blot image from HepG2 cell lysate. For other sample types, check suitability for your experiment.
Source: BosterBio MEIS2 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry O14770.
- Human Protein Atlas. MEIS2 tissue expression.
- PMC6549210 — target-verified WB comparison
- PMC6459881 — target-verified WB comparison
- PMC11074520 — target-verified WB comparison