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- Table of Contents
MGLL / Monoglyceride lipase · Western blot design guide
Design a Western Blot for MGLL
Real validated MGLL Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-MGLL WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
MGLL Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~33.3 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Cerebral cortex (IHC candidate; verify WB) +4 more | |
| Negative control | Esophagus (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 2 isoform(s) |
Section 1
Real Curated MGLL Western Blot Protocols
The M04317-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human heart lysate (catalog M04317-1) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | M04317-1; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected MGLL Western Blot Band Size?
MGLL's predicted mass is 33.3 kDa; homodimerization and splice isoforms could affect bands, but their migration has not been demonstrated here.
| Band near 33.3 kDa | Consistent with the predicted MGLL mass; confirm identity with a specific control. |
| Band near 66.6 kDa | Could reflect an MGLL homodimer if it survives denaturing electrophoresis. |
| Several discrete bands | Could include isoforms 1 and 2, although distinct migration is unverified. |
| Different band intensities across soluble and membrane fractions | Consistent with MGLL's cytosolic and peripheral membrane locations. |
💡Expected MGLL appearanceMGLL has a predicted mass of 33.3 kDa; no empirical band size or isoform migration is supplied, so confirm any candidate band with ordinary identity controls.
| Predicted MGLL mass | Places the reference band near 33.3 kDa. |
| MGLL homodimer | Could appear near twice the monomer mass if it survives denaturing electrophoresis. |
| Splice isoform 1 | May migrate differently from isoform 2; its relative size is unknown. |
| Splice isoform 2 | May migrate differently from isoform 1; its relative size is unknown. |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Membrane-associated MGLL may be poorly recovered by the extraction method. | Check extraction conditions and compare soluble and membrane fractions. |
| Band higher than expected | An MGLL homodimer may persist during electrophoresis. | Compare denaturing conditions and verify band identity with an MGLL control. |
| Band lower than expected | A different splice isoform is possible, but its size is unknown. | Check band identity with an isoform-specific control. |
| Multiple bands | Isoforms 1 and 2 or a persistent homodimer could contribute. | Compare denaturing conditions and use isoform-specific controls. |
| Weak or no signal | MGLL may be distributed between soluble and membrane fractions. | Examine both fractions and include a positive lysate control. |
Experiment evidence
Western blot images showing MGLL band
M04317-1 · MGLL
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for MGLL Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for MGLL in Western blot, you can use cerebral cortex lysate, which HPA rates as highly positive.
Positive control: Cerebral cortex (IHC candidate; verify WB)
Negative control: Esophagus (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: Tissue lysate controls are feasible because HPA reports high expression in cerebral cortex and no detection in esophagus.
HPA tissue expression evidence for MGLL
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cerebral cortex | glial cells | High | Protein (IHC) | HPA → |
| Colon | glandular cells | High | Protein (IHC) | HPA → |
| Small intestine | glandular cells | High | Protein (IHC) | HPA → |
| Adipose tissue | adipocytes | Medium | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Esophagus | squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
| Heart muscle | cardiomyocytes | Not detected | Protein (IHC) | HPA → |
| Oral mucosa | squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
| Ovary | ovarian stroma cells | Not detected | Protein (IHC) | HPA → |
| Seminal vesicle | glandular cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced MGLL Western Blot Tips
Deeper troubleshooting and optimisation questions for MGLL, answered from its protein features.
Where should the main MGLL band appear?
Band shift · The predicted mass of canonical MGLL is 33.3 kDa. No observed Western blot band position is supplied, so use this as a reference rather than an expected apparent mass. The listed modifications alone do not establish a visible shift.
Could MGLL isoforms produce different bands?
Isoforms · UniProt lists isoforms 1 and 2. In isoform 2, the initial M is replaced by METGPEDPSSM and residues 161–190 are missing, making it 20 residues shorter overall. Check whether the antibody epitope is retained before assigning a second band to isoform 2.
Decide whether to quantify isoforms together or separately. Isoform 2 lacks canonical residues 161–190 and has a changed N terminus, so antibody epitope placement can affect which isoforms contribute to the signal. Report the band or bands included in the measurement.
Decide whether to quantify isoforms together or separately. Isoform 2 lacks canonical residues 161–190 and has a changed N terminus, so antibody epitope placement can affect which isoforms contribute to the signal. Report the band or bands included in the measurement.
How should phosphorylation affect MGLL band interpretation?
PTM · UniProt lists phosphothreonine at position 10 of the canonical sequence. An antibody sensitive to this site may detect a different fraction of MGLL, but the feature alone does not establish a visible mobility shift. Check the numbering convention when comparing isoforms or antibody documentation.
Does this guide establish induction of MGLL?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for MGLL?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M04317-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should MGLL be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
What does the MGLL nitration site mean for blot design?
Interpretation · UniProt lists 3'-nitrotyrosine at canonical position 58. For a modification-specific assay, verify that the antibody targets this modification and that its epitope is present in the isoform being measured. The listed site alone does not establish a separate band.
UniProt places MGLL in the cytosol and at membranes as a peripheral membrane protein. Consider both soluble and membrane-associated fractions when assessing recovery; a weak band in one fraction alone may underrepresent total MGLL.
Compare bands with the 33.3 kDa predicted canonical mass and consider isoform 2's sequence changes. UniProt also describes MGLL as a homodimer, but that feature alone does not establish a dimer band on a Western blot. Without an observed band position or validation data, these features cannot identify an unexpected band.
UniProt places MGLL in the cytosol and at membranes as a peripheral membrane protein. Consider both soluble and membrane-associated fractions when assessing recovery; a weak band in one fraction alone may underrepresent total MGLL.
Compare bands with the 33.3 kDa predicted canonical mass and consider isoform 2's sequence changes. UniProt also describes MGLL as a homodimer, but that feature alone does not establish a dimer band on a Western blot. Without an observed band position or validation data, these features cannot identify an unexpected band.
Boster reagents
MGLL Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Monoacylglycerol Lipase Rabbit Monoclonal Antibody
Cat # M04317-1
The listed anti-MGLL rabbit monoclonal antibody has reported human reactivity and a Western blot image showing Monoacylglycerol Lipase expression in human heart lysate. The supplied evidence covers this tested context only.
Which to pick: M04317-1 is the only listed option. Its reported reactivity is human, and its Western blot image uses human heart lysate; consider whether that sample context fits your experiment.
Source: BosterBio MGLL gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q99685.
- Human Protein Atlas. MGLL tissue expression.
- PMC6980785 — target-verified WB comparison
- PMC9780207 — target-verified WB comparison
- PMC7255032 — target-verified WB comparison