MOK / MAPK/MAK/MRK overlapping kinase · Western blot design guide

Design a Western Blot for MOK

Real validated MOK Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-MOK WB antibodies. Everything you need to plan the experiment before you commit precious samples.

Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for MOK: expected band ~48 kDa, hero antibody A01004-4, catalog values and labelled standard workflow; separate PMC comparisons on the guide — Printable **MOK** Western blot protocol sheet — expected band ~48 kDa, antibody A01004-4, controls and PMC citations. Open the full MOK WB guide →

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MOK Western Blot Experimental Design Guide

Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	~48 kDa
	Observed band	~48 kDa
	Gel	10% (catalog A01004-4)
	Negative control ⓘ	Suggested KO / knockdown lysate

Important caveats

Reasons your observed band may differ from the expected size.

	PTM	Phosphorylated
	Caveat	Phosphorylation-state controls
	Gene-set association	MSigDB C7 membership
	Isoform	6 isoform(s)

Section 1

Real Curated MOK Western Blot Protocols

The A01004-4 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.

Recommended Western blot protocol parameters
Sample / lysate	human PC-3, human A549, human 293T (catalog A01004-4)
Gel %	10% (catalog A01004-4)
Load	30 ug; reducing conditions (catalog A01004-4)
Transfer	a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A01004-4)
Membrane	nitrocellulose membrane (catalog A01004-4)
Blocking	5% non-fat milk/TBS for 1.5 hour at RT (catalog A01004-4)
Primary antibody	A01004-4 · 0.5 μg/mL (catalog A01004-4)
Primary incubation	overnight at 4°C (catalog A01004-4)
Secondary antibody	goat anti-rabbit IgG-HRP, 1:5000 (catalog A01004-4)
Secondary incubation	1.5 hour at RT (catalog A01004-4)
Wash	TBS-0.1%Tween 3 times with 5 minutes each (catalog A01004-4)
Detection	ECL (catalog A01004-4)

Section 2

What Is the Expected MOK Western Blot Band Size?

MOK is predicted at 48 kDa and observed at ~48 kDa; six annotated isoforms have no supplied individual band sizes.

What am I looking at on my blot?
Band at ~48 kDa	Matches the predicted mass and the observed MOK band.
Single sharp band near ~48 kDa	Consistent with the reported MOK band; other isoforms need not resolve separately.
Additional bands at different positions	Could reflect alternative isoforms, but their migration has not been established.
No distinct secondary bands	Does not rule out the six annotated isoforms.

💡Expected MOK appearanceMOK has a predicted mass of 48 kDa and an empirical band at ~48 kDa in reducing whole-cell lysates; confirm band identity with an appropriate control.

How each factor affects band size
Predicted MOK mass	Predicts 48 kDa, matching the observed ~48 kDa band.
Splice isoforms 1 and 2	May differ in apparent size; their individual migration is unknown.
Splice isoforms 3 and 4	May differ in apparent size; their individual migration is unknown.
Splice isoforms 5 and 6	May differ in apparent size; their individual migration is unknown.

Why is my band missing or off?
Situation	Likely cause	Next action
No band in lysate	MOK signal may be below detection in the sampled lysate.	Check lysate quality and use a positive control with a verified ~48 kDa MOK band.
Band higher than expected	An alternative isoform is possible, but its migration is unknown.	Compare with the ~48 kDa reference and verify identity by MOK depletion.
Band lower than expected	An alternative isoform is possible, but its migration is unknown.	Check sample integrity and verify identity by MOK depletion.
Multiple bands	Six MOK splice isoforms are annotated, without established band positions.	Use MOK depletion to identify specific bands.
Weak or no signal	MOK may be scarce in the sampled cellular material.	Check loading and detection, then compare with a positive-control lysate.

Source: UniProt Q9UQ07 Human Protein Atlas product datasheet (A01004-4)

Experiment evidence

Western blot images showing MOK band

A01004-4 · MOK

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Sample controls for MOK Western blot

🧪For positive controls for MOK in Western blot, you can use no HPA-supported sample because no positive tissue or cell is supplied.

Positive control: No high/medium HPA tissue identified

Negative control: Suggested KO / knockdown lysate

Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.

⚠️Feasibility: HPA provides no expression data, so suitable positive and negative samples cannot be identified from the supplied evidence.

HPA tissue expression evidence for MOK

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.

Higher expression tissues · candidate positive controls from IHC

Tissue	Cell type	Level	Evidence	Source
No high/medium HPA tissues identified in the supplied evidence.

Lower expression tissues · IHC evidence, not confirmed WB-negative controls

Tissue	Cell type	Level	Evidence	Source
No lower-expression tissue rows available in the supplied evidence.

Section 3

Advanced MOK Western Blot Tips

Deeper troubleshooting and optimisation questions for MOK, answered from its protein features.

How should MOK band migration be interpreted?

Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.

Could MOK isoforms produce bands at different sizes?

Isoforms · UniProt lists six isoforms. Relative to the 419-residue canonical sequence, isoform 2 lacks residues 232–419, isoform 3 lacks 1–346, and isoform 5 lacks 42–71. These sequence differences could change apparent band positions, but the features do not establish which isoforms are expressed or detected.

Check the antibody epitope against each isoform. In UniProt canonical coordinates, isoform 4 lacks residues 1–234 and 330–419 and replaces 290–329; isoform 6 lacks residue 138. An antibody targeting an absent or altered region may miss that isoform.

Does phosphorylation explain a shifted MOK band?

PTM · MOK is annotated as a phosphoprotein, but the supplied features list no modified residue coordinates. Phosphorylation is therefore a possibility to investigate, not an explanation established by these features. Compare treated and control samples before assigning a shifted band to phosphorylation.

Does this guide establish induction of MOK?

Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.

What transfer method to use for MOK Western blot?

Transfer · MOK is predicted at 48 kDa, so use transfer conditions suitable for a protein near that size. Check transfer efficiency around 48 kDa with a total-protein stain or marker before interpreting a weak signal.

How should blocking be optimized?

Blocking · Standard workflow guidance: follow the A01004-4 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.

How should multiple MOK bands be quantified?

Quantitation · Quantify a consistently identified band separately from other bands. The observed band is approximately 48 kDa, while the six annotated isoforms have different sequences. Do not combine bands as total MOK unless their identities and antibody recognition are established.

Should MOK appear at its predicted 48 kDa mass?

Interpretation · The supplied observed band is approximately 48 kDa, matching the predicted mass. Use that position as a reference, but confirm identity with an appropriate control; apparent mass alone is insufficient.

First compare their positions with the approximately 48 kDa observed band and check antibody epitope coverage across the six isoforms. UniProt lists substantial sequence deletions in several isoforms, but those features alone do not identify an unexpected band or prove a visible shift.

Boster reagents

MOK Western Blot Antibodies

Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.

Real WB data

Western blot analysis of MOK using anti-MOK antibody (A01004-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOK antigen affinity purified polyclonal antibody (A01004-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MOK at approximately 48 kDa. The expected band size for MOK is at 48 kDa.

Anti-MOK Antibody Picoband®

Cat # A01004-4

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A01004-4 is a human-reactive anti-MOK antibody with a Western blot image showing an approximately 48 kDa band in PC-3, A549, and 293T whole-cell lysates. The supplied evidence includes no independent publication validation.

Which to pick: A01004-4 is the only listed option. Its WB image uses human cell lysates, making it the documented choice for those sample contexts; performance in other samples is not established here.

Source: BosterBio MOK gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.

References

UniProt Consortium. UniProt entry Q9UQ07.
Human Protein Atlas. MOK tissue expression.
PMC3820702 — target-verified WB comparison
PMC4171540 — target-verified WB comparison