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- Table of Contents
MOV10 / Helicase MOV-10 · Western blot design guide
Design a Western Blot for MOV10
Source-linked MOV10 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-MOV10 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
MOV10 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~113.7 kDa | |
| Observed band | ~114 kDa | |
| Gel | 5–20% (catalog A04952) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 3 isoform(s) |
Section 1
Source-Linked MOV10 Western Blot Protocol Options
The A04952 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human Hela, human 293T, human HepG2, human Jurkat (catalog A04952) |
| Gel % | 5–20% (catalog A04952) |
| Load | 30 ug; reducing conditions (catalog A04952) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A04952) |
| Membrane | nitrocellulose membrane (catalog A04952) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A04952) |
| Primary antibody | A04952 · 0.5 μg/mL (catalog A04952) |
| Primary incubation | overnight at 4°C (catalog A04952) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A04952) |
| Secondary incubation | 1.5 hour at RT (catalog A04952) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A04952) |
| Detection | ECL (catalog A04952) |
Section 2
What Is the Expected MOV10 Western Blot Band Size?
MOV10 is predicted at 113.7 kDa and observed at ~114 kDa; the supplied evidence does not establish a cause for the small difference.
| Band at ~114 kDa | Matches the empirical MOV10 band and the 113.7 kDa predicted mass. |
| Single sharp band near ~114 kDa | Consistent with MOV10; the three named isoforms are not known to resolve separately. |
| Several bands at different sizes | MOV10 isoforms 1, 2 and 3 are possible contributors; band identity needs confirmation. |
| Band below ~114 kDa | Could represent a MOV10 isoform, but its size and identity are unestablished. |
💡Expected MOV10 appearanceMOV10 has a predicted mass of 113.7 kDa and an empirical band at ~114 kDa; confirm band identity with appropriate controls because isoform migration is not established.
| UniProt predicted mass | Predicts 113.7 kDa, consistent with the empirical ~114 kDa band. |
| Isoform 1 | Its individual size and migration relative to the other isoforms are not supplied. |
| Isoform 2 | Its individual size and migration relative to the other isoforms are not supplied. |
| Isoform 3 | Its individual size and migration relative to the other isoforms are not supplied. |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | MOV10 recovery from the lysate may be poor. | Check lysate loading and recovery with appropriate controls. |
| Band higher than expected | The supplied features do not establish a higher migrating MOV10 species. | Confirm identity with an independent antibody or MOV10 depletion. |
| Band lower than expected | An isoform is possible, but isoform masses are unknown. | Confirm identity with MOV10 depletion and an isoform appropriate antibody. |
| Multiple bands | Isoforms 1, 2 and 3 may contribute, but their migration is uncharacterized. | Use MOV10 depletion to identify specific bands. |
| Weak or no signal | MOV10 recovery or antibody detection may be insufficient. | Check loading, transfer and antibody performance with controls. |
Experiment evidence
Western blot images showing MOV10 band
A04952 · MOV10
M04952 · MOV10
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for MOV10 Western blot
🧪For positive controls for MOV10 in Western blot, you can use a validated MOV10-expressing cell lysate; no HPA positive tissue or cell line is supplied.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: HPA supplies no tissue or cell candidates, so tissue controls cannot be selected from this evidence.
HPA tissue expression evidence for MOV10
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced MOV10 Western Blot Tips
Deeper troubleshooting and optimisation questions for MOV10, answered from its protein features.
How should MOV10 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could MOV10 isoforms produce different bands?
Isoforms · Yes. Isoform 2 replaces residues 838–900 and lacks 901–1003; isoform 3 changes 47–56 and lacks 57–1003. These sequence differences could affect band size and antibody recognition. The supplied features do not establish where either isoform migrates.
Check the epitope against the listed alternative sequences. An epitope within 901–1003 is absent from isoforms 2 and 3; one within 57–837 is absent from isoform 3. Residues 47–56 and 838–900 differ in isoforms 3 and 2, respectively.
Check the epitope against the listed alternative sequences. An epitope within 901–1003 is absent from isoforms 2 and 3; one within 57–837 is absent from isoform 3. Residues 47–56 and 838–900 differ in isoforms 3 and 2, respectively.
Which MOV10 modifications matter when interpreting bands?
PTM · UniProt lists N6-acetyllysine at 148, phosphothreonine at 160 and 254, and phosphoserine at 432, 969 and 977. These are UniProt canonical-sequence coordinates; antibody or paper numbering may differ. Their presence alone does not establish a visible shift or explain an apparent mass difference.
Does this guide establish induction of MOV10?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for MOV10 Western blot?
Transfer · MOV10’s canonical predicted mass is 113.7 kDa. Choose and verify transfer conditions that recover proteins around 114 kDa on the membrane; check transfer before interpreting a weak or missing MOV10 signal. The supplied features do not specify a particular transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A04952 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
What should I consider when quantifying MOV10?
Quantitation · Keep the sampled fraction consistent: MOV10 is listed in the cytoplasm, P-bodies and cytoplasmic ribonucleoprotein and stress granules, with a nuclear annotation. If comparing isoforms, confirm that the antibody epitope is present in each isoform being measured.
How does MOV10’s observed band compare with its predicted mass?
Interpretation · The reported band at ~114 kDa agrees with the predicted 113.7 kDa for canonical MOV10. The listed modifications do not, by themselves, establish a visible band shift.
Compare their sizes with canonical MOV10 near 114 kDa and consider the listed isoform sequence changes. Check whether the antibody epitope is retained in each isoform. The six listed modification sites identify possible molecular differences, but do not prove that a particular extra band results from a modification.
Compare their sizes with canonical MOV10 near 114 kDa and consider the listed isoform sequence changes. Check whether the antibody epitope is retained in each isoform. The six listed modification sites identify possible molecular differences, but do not prove that a particular extra band results from a modification.
Boster reagents
MOV10 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-MOV10 Antibody Picoband®
Cat # A04952
Real WB data
Anti-Mov10 Rabbit Monoclonal Antibody
Cat # M04952
Both listed anti-MOV10 antibodies have Western blot images. A04952 shows an approximately 114 kDa band in HeLa, 293T, HepG2 and Jurkat lysates. M04952 shows results in 293 and NIH/3T3 lysates. The images document these tested samples only.
Which to pick: For human samples, A04952 has a detailed image across four human cell lysates. M04952 lists human, mouse and rat reactivity, with images from 293 and NIH/3T3 lysates; the supplied image does not show a rat sample.
Source: BosterBio MOV10 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q9HCE1.
- Human Protein Atlas. MOV10 tissue expression.
- PMC5492891 — target-verified WB comparison
- PMC8214447 — target-verified WB comparison