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- Table of Contents
MTERF1 / Transcription termination factor 1, mitochondrial · Western blot design guide
Design a Western Blot for MTERF1
Source-linked MTERF1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-MTERF1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled October 2026 · For research use; verify linked source records and product datasheet before use
MTERF1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~45.8 kDa | |
| Observed band | 72 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Adrenal gland (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated | |
| Caveat | Peptide-blocking control | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked MTERF1 Western Blot Protocol Options
The A30769 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | the lysates from HUVECcells (catalog A30769) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A30769; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected MTERF1 Western Blot Band Size?
MTERF1 is predicted at 45.8 kDa, while antibody QC reports a 72 kDa band; the cause of the difference is not established.
| Band near 72 kDa | Matches the empirical MTERF1 band; confirm its identity with antibody controls |
| Band near 45.8 kDa | Near the predicted mass; confirm its identity |
| One dominant band | Consistent with the annotated monomer and single isoform |
| Weak band in whole-cell lysate | Mitochondrial localization may limit detection |
💡Expected MTERF1 appearanceMTERF1 has a predicted mass of 45.8 kDa and an empirical band at 72 kDa; the cause of the difference is not established, so confirm band identity with antibody controls.
| UniProt predicted mass | Provides a sequence-based reference of 45.8 kDa |
| Predicted mass in daltons | 45,778 Da rounds to 45.8 kDa |
| Predicted mass versus observed migration | The empirical band is at 72 kDa, with no established cause for the difference |
| Predicted mass as a band reference | A band near 45.8 kDa would require identity confirmation |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Mitochondrial MTERF1 may be scarce in whole-cell lysate | Check a mitochondrial-enriched fraction and a positive-control lysate |
| Band higher than expected | The reported 72 kDa band exceeds the 45.8 kDa prediction for an unknown reason | Check peptide competition and confirm with an independent antibody |
| Band lower than expected | Band identity or sample integrity is uncertain | Check sample handling and confirm with an independent antibody |
| Multiple bands | Only one isoform is annotated; additional band identities are uncertain | Compare peptide-blocked and unblocked lanes |
| Weak or no signal | MTERF1 abundance in the sampled lysate may be low | Use a positive-control lysate or mitochondrial enrichment |
Experiment evidence
Western blot images showing MTERF1 band
A30769 · MTERF1
A30769 · MTERF1
A08459-1 · MTERF1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for MTERF1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for MTERF1 in Western blot, you can use adrenal gland lysate, which has medium HPA signal.
Positive control: Adrenal gland (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: MTERF1 is mitochondrial, so retain mitochondria when preparing the positive-control lysate.
HPA tissue expression evidence for MTERF1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | glandular cells | Medium | Protein (IHC) | HPA → |
| Appendix | glandular cells | Medium | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Medium | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | Medium | Protein (IHC) | HPA → |
| Caudate | neuronal cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Lymph node | germinal center cells | Not detected | Protein (IHC) | HPA → |
| Oral mucosa | squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
| Ovary | ovarian stroma cells | Not detected | Protein (IHC) | HPA → |
| Parathyroid gland | glandular cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced MTERF1 Western Blot Tips
Deeper troubleshooting and optimisation questions for MTERF1, answered from its protein features.
How should MTERF1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could an alternative isoform explain another MTERF1 band?
Isoforms · The supplied record lists one isoform and no alternative sequence. Do not assign a second band to a documented MTERF1 isoform on this evidence alone.
Does phosphorylation explain the 72 kDa band?
PTM · MTERF1 has a phosphoprotein keyword, but no modified residue or site is listed. That annotation alone does not demonstrate a visible band shift or explain the difference between 45.8 and 72 kDa.
Does this guide establish induction of MTERF1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for MTERF1 Western blot?
Transfer · The features do not specify a transfer method. Check transfer at both the predicted 45.8 kDa position and the reported 72 kDa position before interpreting a missing or unexpected signal.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A30769 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should MTERF1 bands be quantified?
Quantitation · Define the band used for quantitation consistently across samples. MTERF1 is annotated as mitochondrial and monomeric, but these features do not establish that a 72 kDa band represents the monomer. Confirm the band's identity before comparing its intensity.
Why is the observed MTERF1 band 72 kDa instead of 45.8 kDa?
Interpretation · The supplied predicted mass is 45.8 kDa, while the reported apparent band is 72 kDa. The listed features do not establish the cause of this difference. Verify band identity before assigning the 72 kDa signal to MTERF1.
MTERF1 is mitochondrial and has a transit-peptide keyword, but no cleavage position is supplied. Import processing may affect mass; these features cannot establish that it produces the 72 kDa band.
The record lists a monomer, one isoform, and no glycosylation sites or modified residue positions. These features do not identify additional bands. Check band identity before attributing them to processing or modification.
MTERF1 is mitochondrial and has a transit-peptide keyword, but no cleavage position is supplied. Import processing may affect mass; these features cannot establish that it produces the 72 kDa band.
The record lists a monomer, one isoform, and no glycosylation sites or modified residue positions. These features do not identify additional bands. Check band identity before attributing them to processing or modification.
Boster reagents
MTERF1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-MTERF Antibody
Cat # A30769
Real WB data
Anti-MTERF MTERF1 Antibody
Cat # A08459-1
The catalog reports two anti-MTERF1 antibodies, A30769 and A08459-1, with stated Human, Mouse, and Rat reactivity. Both have Western blot images. The captions document specific cell lysates; they do not establish performance across all samples of those species.
Which to pick: For HUVEC lysates, A30769 has a matching WB example. A08459-1 has WB examples using HeLa, Raw264.7, and PC12 lysates at 1:500. Choose by the sample and reported conditions; both list Human, Mouse, and Rat reactivity.
Source: BosterBio MTERF1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q99551.
- Human Protein Atlas. MTERF1 tissue expression.
- PMC10941516 — target-verified WB comparison
- PMC8606567 — target-verified WB comparison
- PMC10692114 — target-verified WB comparison