This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
1 Q&As
Facts about Microsomal triglyceride transfer protein large subunit.
.
Human | |
---|---|
Gene Name: | MTTP |
Uniprot: | P55157 |
Entrez: | 4547 |
Belongs to: |
---|
No superfamily |
88kD); 88kDa); ABL; MGC149819; microsomal triglyceride transfer protein large subunit; microsomal triglyceride transfer protein; MTPMGC149820
Mass (kDA):
99.351 kDA
Human | |
---|---|
Location: | 4q23 |
Sequence: | 4; NC_000004.12 (99564078..99623997) |
Liver and small intestine. Also found in ovary, testis and kidney.
Endoplasmic reticulum. Colocalizes with P4HB/PDI in the endoplasmic reticulum (PubMed:23475612, PubMed:26224785).
Boster Bio Anti MTTP Marker is a catalog number A01715. It is a mutagenic marker for humans as well as Rats, Mouses and Humans. It can be stored at 4°C over a month or at -20°C for one year. All Boster Bio antibodies have been validated with positive and negative samples. They are also labeled "anti-MTTP"
The MTTP Marker is a universal marking device utilized in the printing industry. Its unique design permits easy identification of the beginning and end points of the path. The marker is constructed of plastic resin, which is heated and forced into an mold. Once it has been molded, it cools and hardens. The barrels, plugs, caps, and caps of markers are made in the same manner. The nib is made from a mix of water and powder. It is molded into the desired shape, then baked to form the shape of a pointed or flat.
The MTTP Marker has many uses that include highlighting important log statements. For example, persistence related logs may be marked with "DB". The logs can be spread across multiple class files. You can mark the statement with "DB", using the filters specified in logback documentation. Other log analysis tools allow you to filter using the markers. The MTTP Marker can be used as a preprocessor.
The MTTP gene is a marker of cell type and plays a vital role with the transcription of many other genes. These genes are often expressed in tumor cells. These markers can be used to identify the cells that are involved in a specific process. This gene may be positive or negative in one cell type but not in another. The MTTP marker is widely utilized in the field of diagnosis of cancer.
J-N ELISA was used to determine the protein expression level of SO-RB50 cells. The relative levels of expression of IL-8, IL-6 were measured in SO-RB50 cells as well as Y79 cells using identical protocols. The results were compared with the control and vector groups. A similar analysis was performed using SCD (steroyl-CoA desaturase) and GSEA (gene set enrichment analysis) methods.
To determine the amount of protein in SORB50 cells, the following protocols were followed:
We employed the Y79 cell line from the American Type Culture Collection as well as SO-RB50 cell lines obtained from the Ophthalmic Center of Sun Yat-sen University. SO-RB50 cells were grown in RPMI-1640 medium with 1% penicillin/streptomycin. Both cell lines were maintained at 37°C in humidified , humidified air with 5 percent CO2.
For immunoassays, anti-IgG and anti-Ago2 beads were employed. We also used the Magna RIP kit, made by Millipore, Billerica, MA for assessing the levels of proteins in SO-RB50 cells. Furthermore, we utilized the Magna RIP kit to test miR-132-3p levels and coprecipitated RNA.
Curcumin is a potent antitumor agent that has been shown to inhibit the growth of cancerous cells. Curcumin treatment decreased the migration rate of SORB50 and the Y79 cell line. Western blot analysis revealed that curcumin inhibited the expression of several apoptotic protein genes. Caspase-3 and -9 were both cleaved significantly in SO-RB50 cells treated by curcumin. These results suggest that curcumin may be a pro-apoptotic agent in Rb cells.
Exosomes were isolated from RB patients. Exosomes were used to examine RB cell expansion, invasion, and Apoptosis. qRTPCR was utilized to detect the expression of circRNF20 after treatment. Exosomes have been shown to increase cell viability, growth, and colony formation. Exosomes exposed RB cells to an increased expression of circRNF20.
The cells were seeded in Transwell chambers that contained 10 percent FBS or DMEM medium. A tiny pipette tip was used to make an X-shaped mark in each well. The cells were then cultivated in a 37°C incubation chamber with an oxygen concentration of 5. The cells were then incubated at 37°C over 24 hours using 5% CO2. Following that an inverted optical microscope was used to view them. To place the cells during imaging the imaging, a 2 mm square was used.
A multistep approach to tumourigenesis has been utilized to examine the retinoblastoma. Numerous modifications to the Rb1 gene were discovered in the human cell line. In the RB cell line, miRNAs were identified as tumour suppressors and oncogens. However it is necessary to conduct more research to discover how RB tumorigenesis is triggered. The results will aid in the identification of RB treatments.
The study also identified the role played by miR-183 in RB development and progression. Further research is needed to determine if the LRP6/miR-183 axis is involved in the process of tumorigenesis. The study paves the way for novel approaches in treatment for RB. It isn't simple to determine the levels of protein of SO-RB50 cells. The results indicate that LRP6 and miR-183 are responsible for the expression of RB-associated proteins.
For a top-quality, validated anti-MTTP antigen, Boster Bio has a variety of choices. Their antibodies are both monoclonal and polyclonal, and they react with different animal samples. Boster Bio uses mouse and rabbit to develop its P4HB antibodies. This multifunctional protein is responsible for the formation and breaking of the disulfide bond and may also be involved in structural modifications of exofacial and nascent proteins.
PMID: 8111381 by Shoulders C.C., et al. Abetalipoproteinemia is caused by defects of the gene encoding the 97 kDa subunit of a microsomal triglyceride transfer protein.
PMID: 8361539 by Sharp D., et al. Cloning and gene defects in microsomal triglyceride transfer protein associated with abetalipoproteinaemia.