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- Table of Contents
MTTP antibody (A01715-1) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Monkey, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Flow Cytometry analysis of HEPG2 cells using anti-MTTP antibody (A01715-1).
Overlay histogram showing HEPG2 cells stained with A01715-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MTTP Antibody (A01715-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HEPG2 cells stained with A01715-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MTTP Antibody (A01715-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-MTTP Antibody Picoband®
MTTP antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
MTTP antibody was positively detected by WB in:
human HEPG2 whole cell, monkey liver tissue, rat liver tissue, rat small intestine tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MTTP antibody was positively detected by IHC in:
human liver cancer tissue, rat liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
MTTP antibody was positively detected by ICC/IF in:
HEPG2 cell
MTTP antibody was positively detected by FC in:
HEPG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/m
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Monkey
Tested
Recommend
Rat
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-MTTP Antibody Picoband®
View all MTTP Antibodies
View all MTTP Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MTTP recombinant protein (Position: N29-R702).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
99 kDa
Calculated Molecular Weight
99.4 kDa
Background of MTTP
Microsomal triglyceride transfer protein large subunit is a protein that in humans is encoded by the MTTP gene. It is mapped to 4q23. MTP encodes the large subunit of the heterodimeric microsomal triglyceride transfer protein. Protein disulfide isomerase (PDI) completes the heterodimeric microsomal triglyceride transfer protein, which has been shown to play a central role in lipoprotein assembly. Mutations in MTP can cause abetalipoproteinemia.
Cite This Product
Anti-MTTP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01715-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Rabbit
88%
Moderate Homology
Dog
88%
Moderate Homology
Cow
86%
Moderate Homology
Sheep
86%
Moderate Homology
Mouse
85%
Moderate Homology
Rat
85%
Moderate Homology
Zebrafish
53%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MTTP antibody
Immunohistochemistry (IHC) protocol for MTTP antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MTTP antibody
Flow Cytometry (FC) protocol for MTTP antibody
Publications
Application:
Species:
Metabolic characteristics of voriconazole – Induced liver injury in rats
Published: 2023-08-31
Journal: CHEMICO-BIOLOGICAL INTERACTIONS
Application: WB
Impact Factor: 5.10
Species: Rat
PMID: 37659626
Lipodystrophic gene Agpat2 deficiency aggravates hyperlipidemia and atherosclerosis in Ldlr−/− mice
Published: 2023-08-15
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
Application: WB
Impact Factor: 6.20
Species: Mouse
PMID: 37591406
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Product Promise
We promise Anti-MTTP Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A01715-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026