This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
MYF6 / Myogenic factor 6 · Western blot design guide
Design a Western Blot for MYF6
Source-linked MYF6 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-MYF6 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
MYF6 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~27 kDa | |
| Gel | 12–15% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | WB band size unreported | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked MYF6 Western Blot Protocol Options
The A06455 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | THP-1 (40ug), MG63 (20ug), PC3 (10ug), H9C2 (40ug), SP2/0 (20ug) (catalog A06455) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A06455; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected MYF6 Western Blot Band Size?
MYF6 is predicted at 27 kDa; no empirical band is supplied, and its listed features do not demonstrate altered migration.
| Single band near 27 kDa | Consistent with the predicted size of MYF6; confirm identity with controls |
| No band near 27 kDa in whole-cell lysate | Nuclear MYF6 may be difficult to detect in the sampled lysate |
| Band above 27 kDa | Its identity and altered migration are not established by the supplied features |
| Band below 27 kDa | Its identity and altered migration are not established by the supplied features |
💡Expected MYF6 appearanceMYF6 has a predicted mass of 27 kDa, but no empirical band size is supplied; use band-identity controls to assess a signal near that size.
| UniProt predicted mass of 27 kDa | Provides the nominal band-size reference |
| Calculated mass of 26,953 Da | Rounds to approximately 27 kDa |
| Predicted MYF6 mass | Does not establish an empirical migration position |
| Predicted MYF6 mass | Does not establish the identity of bands at other sizes |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Nuclear MYF6 may be insufficient in the sampled whole-cell lysate | Check a nuclear fraction and a suitable positive control |
| Band higher than expected | The supplied features do not establish a size-increasing modification or homodimer band | Confirm identity with an independent antibody or MYF6 depletion |
| Band lower than expected | The supplied features do not establish cleavage or a smaller isoform | Confirm identity with an independent antibody or MYF6 depletion |
| Multiple bands | Only one isoform is listed; the other bands have unconfirmed identities | Check which bands change with MYF6 depletion |
| Weak or no signal | MYF6 may be scarce in the tested sample | Check a suitable positive control and enrich the nuclear fraction |
Experiment evidence
Western blot images showing MYF6 band
A06455 · MYF6
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for MYF6 Western blot
🧪For positive controls for MYF6 in Western blot, you can use a sample with independently confirmed MYF6 expression; the supplied HPA evidence identifies no positive tissue or cell line.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA provides no tissue data, so positive and tissue-negative controls require independent validation.
HPA tissue expression evidence for MYF6
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced MYF6 Western Blot Tips
Deeper troubleshooting and optimisation questions for MYF6, answered from its protein features.
How should MYF6 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Do UniProt isoforms predict multiple MYF6 bands?
Isoforms · The supplied record lists one isoform and no alternative sequence. It therefore provides no isoform-based explanation for multiple bands.
Do annotated modifications prove a band shift?
PTM · The linked UniProt record describes protein features. A modification annotation alone does not demonstrate a visible shift; retain any condition or experimental qualifier attached to it.
Does this guide establish induction of MYF6?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for MYF6 Western blot?
Transfer · Choose a transfer setup validated for proteins around the predicted 27 kDa size, and verify transfer and retention on the membrane. The supplied features do not specify a particular transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A06455 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should MYF6 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
Should MYF6 migrate exactly at its predicted 27 kDa?
Interpretation · Use 27 kDa as a reference, not an exact band position. No observed band size is supplied, so these features cannot establish an apparent mass or explain a difference from the prediction.
MYF6 is listed as nuclear. A nuclear fraction may help assess a weak signal; compare it with a whole-cell sample and check fraction quality before interpreting enrichment.
Efficient DNA binding requires dimerization with another bHLH protein, and MYF6 interacts with CSRP3. Those interactions alone do not establish that a higher-mass species will survive Western-blot preparation; verify the band before assigning it to a complex.
Compare them with the 27 kDa prediction and test whether they track with MYF6-specific detection. The record lists no alternative sequence or modified residues, and its listed features alone cannot identify an unexpected band.
MYF6 is listed as nuclear. A nuclear fraction may help assess a weak signal; compare it with a whole-cell sample and check fraction quality before interpreting enrichment.
Efficient DNA binding requires dimerization with another bHLH protein, and MYF6 interacts with CSRP3. Those interactions alone do not establish that a higher-mass species will survive Western-blot preparation; verify the band before assigning it to a complex.
Compare them with the 27 kDa prediction and test whether they track with MYF6-specific detection. The record lists no alternative sequence or modified residues, and its listed features alone cannot identify an unexpected band.
Boster reagents
MYF6 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Myf-6 (R150) Antibody
Cat # A06455
A06455 is an anti-MYF6 antibody listed for human, mouse, and rat reactivity. Its Western blot image shows five named cell lysates tested at 1:1000 dilution; no publication evidence is supplied.
Which to pick: A06455 is the only listed option. It has a Western blot image using THP-1, MG63, PC3, H9C2, and SP2/0 whole cell lysates; check whether those tested samples and conditions fit your experiment.
Source: BosterBio MYF6 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P23409.
- Human Protein Atlas. MYF6 tissue expression.
- PMC6940881 — target-verified WB comparison
- PMC11481675 — target-verified WB comparison
- PMC7010284 — target-verified WB comparison