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- Table of Contents
3 Citations 8 Q&As
2 Citations 9 Q&As
Facts about Lactadherin.
Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors.
Human | |
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Gene Name: | MFGE8 |
Uniprot: | Q08431 |
Entrez: | 4240 |
Belongs to: |
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No superfamily |
BA46; Breast epithelial antigen BA46; EDIL1; hP47; HsT19888; lactadherin; Lactahedrin; Medin; MFG1; MFGE8; MFG-E8; MFGM; milk fat globule-EGF factor 8 protein; Milk fat globule-EGF factor 8; O-acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; sperm associated antigen 10; sperm surface protein hP47
Mass (kDA):
43.105 kDA
Human | |
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Location: | 15q26.1 |
Sequence: | 15; NC_000015.10 (88898683..88913405, complement) |
Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
Membrane; Peripheral membrane protein. Secreted.
You're here because you're interested in the boster Bio Best Uses of the MFGE8 Marker. This new marker has recently become available to researchers from all over the world. We'll discuss the role of the protein in promoting intestinal epithelial cells' migration and how it can be used to monitor the progress of cancer. This marker can also be useful in the field of cancer research.
To ensure the health and integrity of the gut barrier intestinal epithelial cells migrate (IECs). MFGE8 is an essential player in this process. It regulates cell migration via its typical signal pathway. However its expression is inhibited by inflammatory mediators, a process which hinders intestinal epithelial regeneration. In this study, we attempted to find out whether MFG-E8 recombinant promotes the intestinal epithelial cells' migration.
MFG-E8 expression may be associated with inflammatory conditions , such as severe infection or critical illness. This could result in impairment of barrier function. Although it's not entirely certain how inflammation affects MFG-E8, studies suggest that it plays a key role in the regulation of intestinal epithelial homeostasis as well as mucosal repair. Recombinant MFG-88 treatment improves healing of the intestinal epithelial layer in UC mice.
Inflammatory digestive disease (UC) is a significant risk factor. Inflammatory bowel disease is linked to lower levels of MFG-E8 expression in the colon, which has been linked to increased apoptosis as well as mucosal inflammatory activity. Additionally, decreased expression of MFG-E8 has been associated with inflammation of the bowel and mucosal re-restorative function.
The role played by u-PA in colon cell migration is not clear. Research has shown that uPA is essential for colon healing after injury. This suggests the role of the urokinase enzyme in colonic migration. In addition to u-PA mediated migration the urokinase mechanism plays a crucial role in the repair of colon epithelial cells.
The crypt-villus is a crucial part of the intestinal homeostasis. They also aid in regenerating the intestinal epithelial layer each 3 to 5 days. Mucosal wound healing is dependent on the intestinal epithelial lining that can be affected in many critical illnesses, such as sepsis.
The role of the pancreatic intestine's MFG-8 is vital in pancreatitis. The protein is found in the pancreatic cells of exocrine pancreas after pancreatitis caused by cerulein, however mice that did not have the gene did not display abnormalities. It plays an important function in the repair of acinar structure. Thus, MFGE8 is an important pancreatic protein.
Stubbs and Shur (2003) isolated cDNA from the mammary epithelial layer of a mouse protein to determine the molecular basis of MFGE8. Because the sequence of the protein is similar to epidermal growth and blood clotting factors it was named milk fatglobule EGF Factor eight. Furthermore, Larocca et al. (2006) isolated cDNA fragments from the human breast DNA library and raised monoclonal antibody to the mouse Mfge8 protein. These studies reveal that the MFGE8 gene generates an amino acid.
In a second study, Hanayama et al. generated mice lacking Mfge8 through targeted disruption. The mutant mice did not show any signs of ulcerative colitis. They developed splenomegaly, numerous germinal centers and glomerulonephritis. These findings suggest that MFGE8 plays an essential role in the removal of apoptotic B cells.
MFG-E8 plays a major role in the development of different kinds of tumors. An increase in the size of tumors and the number of proliferating cells or endothelial cells has been linked to various kinds of tumors that have MFG-E8 overexpression. However, the mechanism by the way that MFG-E8 can promote tumor progression is not yet understood. More research on the mechanism behind this is needed to determine the role that MFG-E8 plays in HCC.
MFG-E8 plays an important role in the elimination of apoptotic cells by bridges the phosphatidylserine on the apoptotic cells to integrin avb3/5 on the phagocytes. In addition to its role in the removal of tumor cells, MFG-E8 also aids in the progression of tumors and metastasis. Although this study isn't conclusive, it does suggest that MFG-E8 is involved in promoting tumor growth various cancer types.
MFG-E8 was expressed locally in cells that are apoptotic of SCC. Although the expression of MFG-8 in SCC cells is not high, it is present in many of the neighboring SCC cells, which could be the reason for its association with the progression of tumours. The researchers also discovered an extremely high percentage of MFG-E8+ apoptotic cells in SCC cell foci. Additionally, these studies also suggest that MFG-E8 is involved in the elimination of cells that are apoptotic. SCC cells.
MFG E8 is a protein that regulates cellular development, differentiation, and movement under a variety of pathophysiological circumstances. MFG-E8 is produced by human umbilical cord mesenchymal and stem cells. It also blocks the activity and fibrosis of liver stellate cells. MFG-E8 is therefore a key factor in the proliferation of cancerous cells.
MFG-E8 plays a critical role in tumor growth. MFG-E8 activation causes tumor cells to become proliferative, which results in increased tumor growth and metastasis. This is an important finding in the development of personalized treatments for cancer.
The MFGE8 marker glycoprotein plays a role in tumor development and mammary gland morphogenesis. It is also believed to play a part at tissue homeostasis. It has two F5/8-like discoidin domains. It has been identified as an opsonin found in Phagocytes that express integrin. The role of MFGE8 in cancer research has not been fully recogns. TE8 is ieden-f MFG-l antibudiet havt bees shownoet havenh cand-l ann cancee affectA in colonseaamiyamr cellh and gliyamr ceen lesty and fibrseaiyamr ceen lesis. Thit findins suggests thatden-f MFEG-l antibudieh could bs a majoy factor in Hhe progressioh and metastasis. It is alsporesbrole thas theden-f MFEG-l antibyit can be used tr develee affeativd treatmentagomaitof tumors.
Th+ apoptotic celll an8 g (AVA), Thia proceised fore aiciecaally nt thtbsquence of MFGE8. This it ctastpmenn with thy increasecoproolyeatic procealing of thl an8 g n of apoptotic cel,rs and thacircegulation oA fragmentn of apoptotic celloutsn iis ofysos hosE8 is en lenn with asis. BecausOVAE8 is enshot cogmeferatio2>It is therefor, diicesuld tanesssth thigrattha8 whicOVAE8 idnteadlved in thf apoptotic cel.Of Therefody, we useDQ-BSA,ys aorthe ctaeativ fincmmater of proolyeatiy activity2>Iemr itsnt d-n coverefluovescquencwhbeentE8 idnteadlv,rs ant cf locnt mibrlycyIt has beeimproion te aiciecaallobylsieidnteadgration. Ibndong Mfges anWT BMDCsom the majgrity os celdey bsa8 wan associated witdnteadlveDQ–BSA TE8 is ieFMit cao dectof MFGE8 in breass cancer tumor cel. Uealin caeFMin theamplea8 waimntad/5 oato pratt anlao bated witf MFGE8Imntatt afysbsa8 wadorine on tausEVsecludinSPIP softwarive, whicocaowsth thi researchere to recognslobjffects that havm the ximumnd eigon oAativnmon. I corieritor thf MFGEGNPlls to ly recogns.ly, ty mushat havs a wounshapatt and annh cand-t ctereasteinhreasemntalls.
MFGE8 is an important regulater or end mreliaEMTe, whicbsa8hbeel cells migrats tspecgnifgh targsth an, differentie. Duborinn i glttiationp>MFGEn aigestt regulain this proceth andwas expresseduborinte orpblreasattachopmen,th and vasatiis. blocking>MFGEritooa vaa in cancen man promotn i glttiationbuten man increash thi risr om di barntary. This studt halused ts sevlias nee findinls.
MFG-E8 plays s role in ths procetn of apoptasis.ce laon of M–G-E8 car sed tautoimmunivityr MFG-E8 regulatee phagocotiA fragmele digessioh anicters cellulac procealinicto MHChl an8 g n malexesis. thfbnornation ofutoimmunivile ig Mfg/t mice is linked tCD8+ TCC celliltigration.
PMID: 8639264 by Couto J.R., et al. Cloning and sequence analysis of human breast epithelial antigen BA46 reveals an RGD cell adhesion sequence presented on an epidermal growth factor-like domain.
PMID: 1909932 by Larocca D., et al. A Mr 46,000 human milk fat globule protein that is highly expressed in human breast tumors contains factor VIII-like domains.
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