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Product Info Summary
| SKU: | A02518-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Milk Fat Globule 1/MFGE8 Antibody Picoband®
SKU/Catalog Number
A02518-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Milk Fat Globule 1/MFGE8 Antibody Picoband® catalog # A02518-3. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Milk Fat Globule 1/MFGE8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02518-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Milk Fat Globule 1/MFGE8 recombinant protein (Position: L24-C387).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02518-3 is reactive to MFGE8 in Human
Observed Molecular Weight
43 kDa
Calculated molecular weight
43.1 kDa
Background of MFGE8
MFGE8(Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al.(1997) mapped the MFGE8 gene to chromosome 15q25. Hanayama et al.(2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al.(2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon(PRKCE)-dependent mechanism.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02518-3 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human milk 5 μl, human milk 5 μl, human milk 25 μl, human milk 25 μl
IHC: human renal cancer tissue, human colorectal adenocarcinoma tissue
Validation Images & Assay Conditions
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Western blot analysis of Milk Fat Globule 1/MFGE8 using anti-Milk Fat Globule 1/MFGE8 antibody (A02518-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human milk 5 μl,
Lane 2: human milk 5 μl,
Lane 3: human milk 2.5 μl,
Lane 4: human milk 2.5 μl.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Milk Fat Globule 1/MFGE8 antigen affinity purified polyclonal antibody (Catalog # A02518-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Milk Fat Globule 1/MFGE8 at approximately 43 kDa. The expected band size for Milk Fat Globule 1/MFGE8 is at 43 kDa.
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IHC analysis of Milk Fat Globule 1/MFGE8 using anti-Milk Fat Globule 1/MFGE8 antibody (A02518-3).
Milk Fat Globule 1/MFGE8 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Milk Fat Globule 1/MFGE8 Antibody (A02518-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of Milk Fat Globule 1/MFGE8 using anti-Milk Fat Globule 1/MFGE8 antibody (A02518-3).
Milk Fat Globule 1/MFGE8 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Milk Fat Globule 1/MFGE8 Antibody (A02518-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Milk Fat Globule 1/MFGE8 Antibody Picoband® (A02518-3)
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