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NPTX2 / Neuronal pentraxin-2 · IHC design guide
Design Immunohistochemistry for NPTX2
Plan chromogenic NPTX2 IHC on paraffin sections using pancreatic endocrine cells as a positive reference (HPA tissue IHC). This guide covers fixation, retrieval and interpretation of cytoplasmic staining, with a catalog antibody IHC range of 2–5 μg/mL (HPA tissue IHC; datasheet A09667-1).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
NPTX2 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Cytoplasmic tissue staining (HPA tissue IHC) | |
| Staining pattern | Cytoplasm: islets, cortical neurons, pituitary, adrenal medulla (HPA tissue IHC) | |
| Antigen retrieval | EDTA pH 8.0 HIER, heat-mediated (datasheet A09667-1) | |
| Positive control | Pancreas+2 more · see all | |
| Negative control | Adipose tissue+4 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Keep paraffin-section fixation consistent. (standard IHC practice; not target-specific) | |
| Caveat | Secreted protein may stain away from its RNA source (HPA tissue IHC) | |
| Regulation | Pituitary-enriched RNA (HPA tissue RNA) | |
| Isoform / epitope | No listed isoforms; mature chain lacks signal peptide 1–15 (UniProt) |
Section 1
Recommended NPTX2 IHC & IF Protocols
The catalog antibody protocol is followed by three published chromogenic IHC protocols for NPTX2, covering paraffin sections and brain sections (PMC7985451; PMC6046040; PMC10813937).
| Sample | Paraffin-embedded mouse pancreas tissue; fixative not specified (datasheet A09667-1) |
| Fixation | Image fixative and duration unreported (datasheet A09667-1); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: EDTA pH 8.0 (datasheet A09667-1); 20 min, 95–100 °C (standard) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% goat serum (datasheet A09667-1) |
| Primary antibody | Rabbit anti-NPTX2, 2-5 μg/ml (datasheet A09667-1) |
| Primary incubation | Overnight at 4 °C (datasheet A09667-1) |
| Detection | HRP-conjugated secondary, DAB chromogen (datasheet A09667-1) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | NPTX2-positive staining in pancreatic endocrine cells of pancreas (HPA tissue IHC: High). HPA tissue profile: Cytoplasmic expression in islets of Langerhans, neuronal cells in cerebral cortex, and in pituitary gland and adrenal medulla. No signal in the no-primary control. |
💡Decision noteStart with heat-mediated EDTA retrieval at pH 8.0 for the catalog antibody (datasheet: A09667-1); published alternatives use citrate or pH 9 retrieval (PMC6046040; PMC10813937).
Section 2
What Is the Expected NPTX2 Staining Pattern?
NPTX2 is secreted and has no transmembrane segment (UniProt P47972 topology). In paraffin-section IHC, expect chiefly cytoplasmic staining in pancreatic endocrine cells and anterior pituitary cells, with weaker staining in adrenal medullary cells and cerebral cortical neurons (HPA tissue IHC). HPA rates the tissue profile Enhanced; because NPTX2 is secreted, its protein location need not match the cells with the most RNA (HPA tissue IHC).
| Strong cytoplasmic staining in pancreatic islets or anterior pituitary cells. | This matches the reported high staining in pancreatic endocrine and anterior pituitary cells (HPA tissue IHC). Assess the named cells and their compartment, rather than treating any staining in the organ as confirmation. |
| Predominantly nuclear staining in cells expected to be positive. | A nuclear-dominant pattern conflicts with the reported cytoplasmic tissue profile (HPA tissue IHC). Treat it as suspect; compare with a known-positive section and the negative control before interpreting it as NPTX2. |
| Strong staining in adipocytes or another cell type reported as unstained. | HPA reports adipocytes as not detected in adipose tissue and breast (HPA tissue IHC). Consider antibody cross-reactivity or endogenous chromogenic detection activity; check staining in a no-primary control (general IHC practice). |
| Diffuse color across cells and surrounding tissue, obscuring cell boundaries. | This does not establish the cell-restricted cytoplasmic pattern reported by HPA (HPA tissue IHC). General IHC practice: review background controls, detection activity, blocking, washes and primary-antibody concentration before scoring. |
| No staining in pancreatic endocrine cells or anterior pituitary cells. | Both are reported high-staining cell populations (HPA tissue IHC). An absent signal makes a negative result elsewhere difficult to interpret; inspect section quality and verify the antibody, retrieval and detection workflow (general IHC practice). |
💡Expected NPTX2 appearanceCall a positive result when cytoplasmic staining is strong in pancreatic endocrine or anterior pituitary cells (HPA: High); nuclear-dominant or broadly uniform staining is suspect against that profile (HPA tissue IHC).
| Secreted protein and processing | NPTX2 has a signal peptide at residues 1–15, a mature chain at 16–431 and no transmembrane segment (UniProt P47972). Interpret the observed cytoplasmic IHC profile without assuming a membrane-bound target (HPA tissue IHC; UniProt P47972 topology). |
| Tissue and cell choice | Pancreatic endocrine and anterior pituitary cells stain High; adrenal medullary cells stain Medium, while cortical neurons stain Low (HPA tissue IHC). Choose the high-staining cells to judge assay sensitivity. |
| RNA–protein location | HPA calls pituitary RNA tissue enriched but cautions that RNA and protein locations can differ for a secreted protein (HPA tissue IHC). Do not score an IHC result solely by RNA abundance. |
| Testis comparison | UniProt reports the highest tissue levels in testis (UniProt P47972 tissue specificity), while HPA records Low staining in pachytene spermatocytes (HPA tissue IHC). Keep assay-specific evidence separate when selecting an IHC control. |
| IHC antibody evidence | HPA049799 and CAB020801 have Enhanced IHC status (HPA antibodies). HPA defines this as a pattern supported by independent antibodies or orthogonal data; it does not establish the performance of every antibody. |
| IF/ICC Q: Should Golgi or filament staining define a tissue IHC positive? | No. HPA reports Golgi apparatus and actin filaments as main ICC-IF locations, with centrosome additional (HPA subcellular). Judge paraffin-section IHC against its tissue cytoplasmic profile (HPA tissue IHC). |
| Situation | Likely cause | Next action |
|---|---|---|
| Pancreatic islets and anterior pituitary both lack signal. | The absence conflicts with their reported High staining (HPA tissue IHC); the failed step is undetermined. | General IHC practice: verify primary-antibody identity and working dilution, antigen retrieval, detection reagents and a known-positive section before scoring negatives. |
| Only adrenal medulla stains faintly. | Adrenal medullary cells have a Medium rating, below the High ratings in pancreatic endocrine and anterior pituitary cells (HPA tissue IHC). | Evaluate a High-rated cell population alongside the section (HPA tissue IHC); use the positive control to decide whether faint staining reflects the assay (general IHC practice). |
| Color appears in adipocytes and in the no-primary control. | Adipocytes are reported Not detected (HPA tissue IHC); color without primary antibody suggests endogenous detection activity (general IHC practice). | General IHC practice: address endogenous chromogenic activity and repeat the no-primary control before attributing adipocyte color to NPTX2. |
| Nuclei stain more strongly than cytoplasm. | This conflicts with the cytoplasmic tissue profile (HPA tissue IHC); its specific cause is unresolved. | General IHC practice: compare a known-positive section and no-primary control, then review antibody concentration and detection conditions before interpretation. |
| The section has diffuse background that hides islet cells. | The reported cell-specific pattern cannot be assessed through widespread color (HPA tissue IHC); several workflow steps can contribute (general IHC practice). | General IHC practice: inspect the no-primary control and review blocking, washes, primary-antibody dilution and chromogen development. |
| Testis is weak despite an expectation of high staining. | UniProt reports highest tissue levels in testis, while HPA rates pachytene spermatocyte IHC staining Low (UniProt P47972; HPA tissue IHC). | Use pancreatic endocrine or anterior pituitary cells, both rated High, to assess the IHC run (HPA tissue IHC); do not infer assay failure from testis alone. |
Experiment evidence
IHC & IF images showing NPTX2 staining
A09667-1 · IHCIHC analysis of NPTX2 using anti-NPTX2 antibody (A09667-1). NPTX2 was detected in a paraffin-embedded section of mouse pancreas tissue. Heat
A09667-1 · IHCIHC analysis of NPTX2 using anti-NPTX2 antibody (A09667-1). NPTX2 was detected in a paraffin-embedded section of mouse pancreas tissue. Heat
A09667 · IHCImmunohistochemistry Validation of NPTX2 in Human Brain Immunohistochemical analysis of paraffin-embedded human brain using anti-NPTX2 antib
A09667 · IHCImmunohistochemistry Validation of NPTX2 in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain issue using ant
A09667 · IFImmunofluorescence Validation of NPTX2 in Human Brain Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labe
HPA · Pituitary glandHuman Protein Atlas IHC, Pituitary gland. Source: https://www.proteinatlas.org/ENSG00000106236-NPTX2/tissue/pituitary+gland#imid_928
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000106236-NPTX2/tissue/cerebral+cortex#imid_20300825
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000106236-NPTX2/tissue/colon#imid_20304631
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000106236-NPTX2/tissue/liver#imid_20304640
![HPA ICC-IF BJ [Human fibroblast] — NPTX2 (HPA058320)](https://images.proteinatlas.org/58320/1742_F8_18_cr5804950376bc9_blue_red_green.jpg)
HPA · ICC-IF BJ [Human fibroblast]Human Protein Atlas immunofluorescence, BJ [Human fibroblast], antibody HPA058320; green = NPTX2, blue = nucleus, red = microtubules.
![HPA ICC-IF BJ [Human fibroblast] — NPTX2 (HPA058320)](https://images.proteinatlas.org/58320/1742_F8_22_cr5804950d083f7_blue_red_green.jpg)
HPA · ICC-IF BJ [Human fibroblast]Human Protein Atlas immunofluorescence, BJ [Human fibroblast], antibody HPA058320; green = NPTX2, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for NPTX2 IHC & IF
🧪Run pancreas first: pancreatic endocrine cells should stain strongly (HPA: High in pancreatic endocrine cells). Use adipose tissue as the negative tissue (HPA: Not detected in adipocytes); on the pancreas slide, assess neighboring non-endocrine cells for background staining rather than assuming they are validated negatives.
Positive control tissue: Pancreas (Pancreatic endocrine cells, HPA High)
Negative control tissue: Adipose tissue (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show NPTX2 in BJ [Human fibroblast], LHCN-M2, U2OS, with annotated localisation: Golgi apparatus (approved), Actin filaments (approved) (HPA subcellular).
Technical controls: Include a no-primary (secondary-only) slide, a concentration-matched rabbit IgG isotype control, and NPTX2-knockout tissue if available (caption: rabbit primary antibody; standard IHC controls). Quench endogenous peroxidase in pancreas sections before HRP–DAB detection (caption: HRP–DAB detection; standard IHC practice).
⚠️Feasibility: The selected A09667-1 paraffin-section caption does not report a fixative or a target-specific fixation window (caption: fixative unreported). It uses heat retrieval in EDTA at pH 8.0, but does not establish whether retrieval is required; the supplied evidence also does not establish whether frozen sections or IF are easier (caption: EDTA heat retrieval). Because NPTX2 is secreted, assess extracellular staining cautiously, and use the peroxidase control to identify pancreatic background (UniProt P47972: Secreted; caption: HRP–DAB detection).
HPA tissue IHC evidence for NPTX2
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Enhanced — High consistency between antibody staining and RNA expression data. Secreted protein, tissue location of RNA and protein is expected to differ. External characterization data supports antibody staining.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Pancreas | Pancreatic endocrine cells | High | Protein (IHC) | HPA → |
| Pituitary gland | Cells in anterior | High | Protein (IHC) | HPA → |
| Adrenal gland | Medullary cells | Medium | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | Adipocytes | Not detected | Protein (IHC) | HPA → |
| Appendix | Endocrine cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | Hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Breast | Adipocytes | Not detected | Protein (IHC) | HPA → |
| Bronchus | Respiratory epithelial cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced NPTX2 IHC Tips
For NPTX2 chromogenic IHC in paraffin sections, anchor troubleshooting to the catalog antibody’s pancreas example and interpret staining by cell type and compartment.
Where should I start if NPTX2 staining is weak after antigen retrieval?
Start with heat-mediated antigen retrieval in EDTA at pH 8.0 for paraffin sections (datasheet A09667-1). The catalog example detected NPTX2 in mouse pancreas after this retrieval, followed by 2 μg/ml primary antibody overnight at 4°C (datasheet A09667-1). If staining remains weak, compare retrieval heating times on adjacent sections while holding antibody concentration and DAB development constant (standard IHC practice). Include pancreatic endocrine cells as a reference for the expected signal, and check whether tissue architecture deteriorates as heating increases (HPA: high in pancreatic endocrine cells; standard IHC practice). Record the retrieval conditions alongside each stained section so changes in signal remain interpretable (standard IHC practice).
Could fixation explain weak or uneven NPTX2 staining?
The selected pancreas caption identifies a paraffin-embedded section but does not state its fixative, so target-specific fixation sensitivity is unknown (datasheet A09667-1). Document the fixative and fixation duration for each specimen before comparing NPTX2 staining across blocks (standard IHC practice). If staining differs between blocks, compare sections processed with the same retrieval, 2 μg/ml primary antibody, and DAB development conditions used in the catalog example (datasheet A09667-1; standard IHC practice). Review morphology and staining of a reference section from the same processing batch before attributing weak signal to NPTX2 biology (standard IHC practice). Neither the tissue expression profile nor the secreted topology establishes a fixation effect for this antibody (HPA: tissue IHC profile; UniProt P47972 topology).
Which NPTX2 staining pattern should I expect in tissue sections?
Assess NPTX2 within the tissue context: HPA reports cytoplasmic staining in pancreatic islets, cerebral cortical neurons, pituitary cells, and adrenal medulla (HPA: tissue IHC profile). Pancreatic endocrine cells and anterior pituitary cells show high staining, whereas adrenal medullary cells show medium staining in that profile (HPA: tissue IHC profile). NPTX2 is annotated as secreted and has no transmembrane segment, so a sharp, continuous plasma-membrane outline alone needs scrutiny (UniProt P47972 topology). An intracellular signal can still be compatible with protein passing through the secretory pathway; HPA IF also reports Golgi localisation (UniProt P47972: secreted; HPA: subcellular profile). Compare cell identity, compartment, and nearby tissue rather than treating any brown deposit as specific (standard IHC practice).
How do processing and epitope uncertainty affect NPTX2 IHC?
The record lists 0 isoforms and a signal peptide at residues 1–15, followed by the annotated chain at 16–431 (UniProt P47972). It also lists glycosylation sites at 148, 189, and 393, with a pentraxin domain at 223–424 (UniProt P47972). The supplied product caption does not identify the antibody epitope, so it cannot establish whether processing or glycosylation masks the recognized site (datasheet A09667-1). If retrieval changes staining, compare matched serial sections and their morphology before assigning the difference to epitope accessibility (standard IHC practice). Use the same antibody lot and detection conditions for that comparison, and report epitope identity as unknown (standard IHC practice; datasheet A09667-1).
How should I check an IF result against this chromogenic IHC pattern?
For an IF comparison, multiplex NPTX2 with a marker of the expected cell type, such as a pancreatic endocrine-cell marker when examining islets (HPA: pancreatic endocrine-cell staining; standard IF practice). Choose fluorophores after checking an unstained section for tissue autofluorescence, and keep channels spectrally separated (standard IF practice). Because NPTX2 is secreted and lacks a transmembrane segment, choose permeabilisation according to the compartment being tested: omit it for accessible extracellular antigen and compare a permeabilised condition for intracellular signal (UniProt P47972 topology; standard IF practice). HPA reports Golgi, actin-filament, and additional centrosome localisation in its subcellular IF profile, which can guide compartment checks (HPA: subcellular profile). Score IF alongside the chromogenic section by cell identity and distribution; the IHC caption does not establish an IF protocol (datasheet A09667-1; standard IF practice).
How can I reduce diffuse brown background without losing NPTX2 signal?
First compare a no-primary control with the stained section to identify signal from the detection system or tissue itself (standard IHC practice). The catalog pancreas example used 10% goat serum blocking, 2 μg/ml primary antibody overnight at 4°C, and DAB detection (datasheet A09667-1). If background persists, check peroxidase blocking and shorten DAB development in matched sections while keeping retrieval fixed (standard IHC practice). Compare diffuse deposit with the expected cytoplasmic signal in pancreatic endocrine cells, rather than calling all islet-adjacent staining positive (HPA: pancreatic endocrine-cell staining; standard IHC practice). Inspect section edges, folds, and damaged regions separately because uneven reagent exposure can complicate interpretation there (standard IHC practice).
What should I measure when comparing NPTX2 across paraffin sections? ⚠ ANSWER MARKED FOR VERIFICATION
Define the cell population before scoring: HPA reports high NPTX2 staining in pancreatic endocrine cells and anterior pituitary cells (HPA: tissue IHC profile). For a defined population, report the percentage of positive cells and an H-score based on staining intensity and the percentage at each intensity (standard IHC practice). If measuring stained area or positive-cell density per mm², normalise to the viable area of the relevant compartment rather than the whole section (standard IHC practice). Apply one retrieval, antibody, DAB, and counterstain workflow across comparison groups, using the catalog conditions as a documented starting point (datasheet A09667-1; standard IHC practice). State the positivity threshold and exclude folds, necrosis, and edges consistently before comparing groups (standard IHC practice).
When should apparent NPTX2 positivity be treated as artefact?
Give greatest weight to reproducible cytoplasmic staining in the expected cells, including pancreatic endocrine cells and anterior pituitary cells (HPA: tissue IHC profile). A uniform membrane rim or widespread staining of unrelated cells needs further checking because NPTX2 is secreted and lacks a transmembrane segment (UniProt P47972 topology; standard IHC practice). Compare suspect deposits with a no-primary control and inspect whether signal concentrates at edges, folds, or necrotic areas (standard IHC practice). Check endogenous peroxidase activity when brown DAB signal persists without primary antibody (standard IHC practice). HPA reports enhanced reliability but cautions that tissue locations of RNA and secreted protein can differ, so RNA location alone cannot settle an ambiguous stain (HPA: reliability description).
Boster reagents
Best NPTX2 / Neuronal pentraxin-2 IHC Antibodies
Anti-NPTX2 antibodies have IHC images from human and mouse brain and mouse and rat pancreas; IF images cover human and mouse brain (catalog image captions).
Real IHC data
Anti-NPTX2 Antibody ®
Cat # A09667-1
Real IHC data
Anti-Neuronal pentraxin-2 NPTX2 Antibody
Cat # A09667
A09667 has paraffin-section IHC images from formaldehyde-fixed human and mouse brain and IF images from paraformaldehyde-fixed human and mouse brain (A09667 image captions). A09667-1 has paraffin-section IHC images from mouse and rat pancreas; its captions do not report a fixative (A09667-1 image captions).
Which to pick: For brain IHC, choose A09667 for human or mouse sections (A09667 IHC captions); for mouse or rat pancreas IHC, choose A09667-1, whose paraffin-section captions leave the fixative unreported (A09667-1 IHC captions). For IF, choose A09667 for human or mouse brain; ICC validation is not reported (A09667 IF captions and application list). For work across species, both list human, mouse and rat reactivity, but the documented IHC examples cover human and mouse for A09667 and mouse and rat for A09667-1; clonality is unreported for both (catalog reactivity, image captions and clone fields).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry P47972 (NPTX2_HUMAN, Neuronal pentraxin-2).
- Human Protein Atlas. NPTX2 tissue IHC expression (reliability: Enhanced).
- Human Protein Atlas. NPTX2 subcellular location (ICC-IF): Mainly localized to the Golgi apparatus and actin filaments. In addition localized to the centrosome..
- Human Protein Atlas. NPTX2 antibody validation summary (3 antibodies).
- Overexpression of NPTX2 Promotes Malignant Phenotype of Epithelial Ovarian Carcinoma via IL6-JAK2/STAT3 Signaling Pathway Under Hypoxia. Frontiers in oncology 2021 — PMC7985451.
- NPTX2 is a key component in the regulation of anxiety. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 2018 — PMC6046040.
- Aberrant DNA Methylation of NPTX2 as an Indicator of Malignant Behavior in Thymic Epithelial Tumors. Cancers 2024 — PMC10813937.
- Synergism of Carbamoylated Erythropoietin and Insulin-like Growth Factor-1 in Immediate Early Gene Expression. Life (Basel, Switzerland) 2023 — PMC10532867.
- PubMed PMID:8530029 — UniProt-cited evidence.
- PubMed PMID:12690205 — UniProt-cited evidence.
- PubMed PMID:12853948 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for NPTX2 (Neuronal pentraxin-2). Expected localisation Cytoplasmic tissue staining (HPA tissue IHC). Antigen retrieval: EDTA pH 8.0 HIER, heat-mediated (datasheet A09667-1). Validated IHC antibody: A09667-1 at 2-5 μg/ml (the product's own IHC validation image is shown). Controls (HPA): positive Pancreas (Pancreatic endocrine cells); negative Adipose tissue. IHC-P workflow on paraffin sections; fixative unspecified. Protocol steps: 1. Sample: Paraffin-embedded mouse pancreas tissue; fixative not specified (datasheet A09667-1); 2. Fixation: Image fixative and duration unreported (datasheet A09667-1); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: EDTA pH 8.0 (datasheet A09667-1); 20 min, 95–100 °C (standard); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% goat serum (datasheet A09667-1); 8. Primary antibody: Rabbit anti-NPTX2, 2-5 μg/ml (datasheet A09667-1); 9. Primary incubation: Overnight at 4 °C (datasheet A09667-1). Watch for: Fixation: Keep paraffin-section fixation consistent. (standard IHC practice; not target-specific); Caveat: Secreted protein may stain away from its RNA source (HPA tissue IHC); Isoform / epitope: No listed isoforms; mature chain lacks signal peptide 1–15 (UniProt); Regulation: Pituitary-enriched RNA (HPA tissue RNA). Evidence: UniProt P47972, HPA tissue IHC · Enhanced, HPA ICC-IF · Mainly localized to the Golgi apparatus and actin filaments. In…, Datasheet A09667-1, PMC7985451, PMC6046040. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/NPTX2/immunohistochemistry-ihc-guide.