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- Table of Contents
NUP107 / Nuclear pore complex protein Nup107 · Western blot design guide
Design a Western Blot for NUP107
Source-linked NUP107 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-NUP107 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
NUP107 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~106.4 kDa | |
| Gel | 8–10% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 3 isoform(s) |
Section 1
Source-Linked NUP107 Western Blot Protocol Options
The A03724 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | A549 cell lysate (catalog A03724) |
| Gel % | 8–10% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Wet/tank transfer; optimize duration (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A03724 · (A) 1 and (B) 2 μg/mL (catalog A03724) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected NUP107 Western Blot Band Size?
NUP107 is predicted at 106.4 kDa; isoforms and phosphorylation are annotated, but their effects on blot migration have not been demonstrated here.
| Band near 106.4 kDa | Consistent with predicted NUP107 size; confirm identity with antibody controls. |
| Single sharp band | The supplied features do not establish a resolvable shift among NUP107 isoforms. |
| Close doublet | Could reflect phosphorylation states, but a migration difference is unconfirmed. |
| Several discrete bands | Could include isoforms 1, 2, and 3; their migration positions are unknown. |
💡Expected NUP107 appearanceNUP107 has a predicted mass of 106.4 kDa, with no empirical band size supplied; use band-identity controls to assess any additional or displaced bands.
| UniProt predicted mass | Places the reference band near 106.4 kDa. |
| Isoform 1 | Its individual mass and migration relative to the other isoforms are unknown. |
| Isoform 2 | Its individual mass and migration relative to the other isoforms are unknown. |
| Isoform 3 | Its individual mass and migration relative to the other isoforms are unknown. |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Nuclear pore-associated NUP107 may be poorly recovered during extraction. | Check the nuclear fraction and extraction efficiency. |
| Band higher than expected | Phosphorylation is annotated, but its effect on migration is unestablished. | Compare phosphatase-treated material and confirm identity with NUP107 depletion. |
| Band lower than expected | An isoform or protein fragmentation could produce a lower band; neither size is supplied. | Compare antibodies against different regions and confirm with NUP107 depletion. |
| Multiple bands | Isoforms 1, 2, and 3 are annotated, but distinct migration is unconfirmed. | Use isoform-aware reagents or NUP107 depletion to identify the bands. |
| Weak or no signal | Nuclear pore-associated protein may be underrepresented in the tested fraction. | Check a nuclear fraction and a suitable positive-control lysate. |
| Fragments below expected size | NUP107 may have degraded during sample preparation. | Prepare fresh lysate with protease inhibitors and compare antibody epitopes. |
Experiment evidence
Western blot images showing NUP107 band
A03724 · NUP107
A03724-1 · NUP107
A03724-1 · NUP107
M03724 · NUP107
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for NUP107 Western blot
🧪For positive controls for NUP107 in Western blot, you can use no HPA-derived sample because no positive tissue or cell data were supplied.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: HPA data are unavailable, so tissue controls cannot be validated; use a knockdown or KO line for a negative control.
HPA tissue expression evidence for NUP107
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced NUP107 Western Blot Tips
Deeper troubleshooting and optimisation questions for NUP107, answered from its protein features.
What band size should I expect for NUP107?
Band shift · The predicted mass of canonical NUP107 is 106.4 kDa. No observed band size is supplied, so use 106.4 kDa as a reference rather than an established migration position.
Could NUP107 isoforms produce different bands?
Isoforms · Yes. UniProt lists three isoforms. Isoform 2 replaces canonical residues 1–63 with a different sequence; isoform 3 lacks residues 1–151 and 579–666. These sequence differences could affect band size. Check which isoforms the antibody can recognize before assigning bands.
Could NUP107 modifications explain a shifted band?
PTM · UniProt lists phosphorylation, arginine methylation, and N-terminal acetylation. Their presence alone does not establish a visible shift or explain a difference from 106.4 kDa. If a shift is observed, assess it experimentally before attributing it to a modification.
In UniProt canonical numbering, phosphoserines are at 4, 10, 11, 37, 57, 58, 69, and 86; phosphothreonines are at 46, 55, and 64. Isoforms 2 and 3 alter the N-terminal region, so account for isoform sequence when comparing site numbers or antibody epitopes.
In UniProt canonical numbering, phosphoserines are at 4, 10, 11, 37, 57, 58, 69, and 86; phosphothreonines are at 46, 55, and 64. Isoforms 2 and 3 alter the N-terminal region, so account for isoform sequence when comparing site numbers or antibody epitopes.
Does this guide establish induction of NUP107?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for NUP107?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A03724 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should NUP107 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
How should NUP107 methylation sites be interpreted?
Interpretation · UniProt lists alternate modifications at canonical Arg60: asymmetric dimethylarginine or omega-N-methylarginine. It also lists omega-N-methylarginine at Arg68. Do not treat the two Arg60 annotations as simultaneous modifications, and account for the altered N-terminal sequence in isoforms 2 and 3.
NUP107 is annotated at the nucleus membrane and nuclear pore complex. Compare like-for-like sample preparations and normalize signals within the same preparation. If an antibody detects multiple isoforms, define which band or bands you quantify and apply that choice consistently.
Compare each band with the 106.4 kDa canonical prediction, then consider the sequence changes in isoforms 2 and 3 and the antibody's epitope. The listed modifications do not, by themselves, identify an unexpected band. No empirical NUP107 band position is supplied here.
NUP107 is annotated at the nucleus membrane and nuclear pore complex. Compare like-for-like sample preparations and normalize signals within the same preparation. If an antibody detects multiple isoforms, define which band or bands you quantify and apply that choice consistently.
Compare each band with the 106.4 kDa canonical prediction, then consider the sequence changes in isoforms 2 and 3 and the antibody's epitope. The listed modifications do not, by themselves, identify an unexpected band. No empirical NUP107 band position is supplied here.
Boster reagents
NUP107 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-NUP107 Antibody
Cat # A03724
Real WB data
Anti-NUP107 Antibody Picoband®
Cat # A03724-1
Real WB data
Anti-Nup107 Rabbit Monoclonal Antibody
Cat # M03724
Three the supplier anti-NUP107 antibodies have product WB images. A03724-1 shows an approximately 106 kDa band across listed human cell, rat PC-12, and mouse testis and NIH/3T3 lysates. The supplied evidence is limited to product captions; no independent publication evidence is provided.
Which to pick: For a human, mouse, or rat sample, consider A03724-1 because its WB image includes named specimens from all three species. A03724 is also listed for all three but shows A549 lysate; human-only M03724 shows 293 cell lysate.
Source: BosterBio NUP107 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P57740.
- Human Protein Atlas. NUP107 tissue expression.
- PMC9025798 — target-verified WB comparison
- PMC11469062 — target-verified WB comparison