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- Table of Contents
OAS1 / 2'-5'-oligoadenylate synthase 1 · Western blot design guide
Design a Western Blot for OAS1
Real validated OAS1 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-OAS1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
OAS1 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~46 kDa | |
| Observed band | ~105 kDa | |
| Gel | 10% (catalog A01684-3) | |
| Positive control | Adipose tissue (IHC candidate; verify WB) +4 more | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | OAS1/3 antibody specificity | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 4 isoform(s) |
Section 1
Real Curated OAS1 Western Blot Protocols
The A01684-3 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human Hela, human A549 (catalog A01684-3) |
| Gel % | 10% (catalog A01684-3) |
| Load | 30 ug; reducing conditions (catalog A01684-3) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A01684-3) |
| Membrane | nitrocellulose membrane (catalog A01684-3) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A01684-3) |
| Primary antibody | A01684-3 · 1:1000 (catalog A01684-3) |
| Primary incubation | overnight at 4°C (catalog A01684-3) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A01684-3) |
| Secondary incubation | 1.5 hour at RT (catalog A01684-3) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A01684-3) |
| Detection | ECL (catalog A01684-3) |
Section 2
What Is the Expected OAS1 Western Blot Band Size?
OAS1 is predicted at 46 kDa; an OAS1/3 antibody detected approximately 105 kDa, but that band’s OAS1 identity and the size difference’s cause are not established.
| Band near 105 kDa | Empirical band reported with an OAS1/3 antibody; OAS1 identity requires confirmation |
| Band near 46 kDa | Consistent with the UniProt predicted OAS1 mass |
| Several bands at different positions | OAS1 splice isoforms may contribute, but their migration is unverified |
| Bands in cytoplasmic and nuclear fractions | Consistent with reported OAS1 localization in both compartments |
💡Expected OAS1 appearanceUniProt predicts OAS1 at 46 kDa, while an OAS1/3 antibody detected a band near 105 kDa; confirm its identity with OAS1-specific and OAS3 controls.
| UniProt predicted mass | Places the reference OAS1 sequence near 46 kDa |
| p46 splice isoform | May differ in size from other isoforms; its migration is unverified |
| p42 splice isoform | May differ in size from other isoforms; its migration is unverified |
| p48 splice isoform | May differ in size from other isoforms; its migration is unverified |
| p44 splice isoform | May differ in size from other isoforms; its migration is unverified |
| OAS1 homotetramer | Could migrate higher if the complex remains intact; this does not establish the 105 kDa band’s identity |
| Situation | Likely cause | Next action |
|---|---|---|
| Band higher than expected | The 105 kDa band was detected by an OAS1/3 antibody; its molecular identity is unresolved | Compare OAS1 and OAS3 depletion controls with an OAS1-specific antibody |
| Band lower than expected | A splice isoform or unrelated antibody target may migrate lower | Check an OAS1 depletion control and an isoform-specific reference |
| Multiple bands | Four OAS1 splice isoforms are listed, but their band positions are unverified | Use isoform-specific references and OAS1 depletion to assign bands |
| No band in lysate | OAS1 expression or detection in the sample is unverified | Check an OAS1-positive control and antibody sensitivity |
| Weak or no signal | OAS1 occurs in several cellular compartments, which may dilute signal in a selected fraction | Compare whole-cell lysate with cytoplasmic and organelle fractions |
Experiment evidence
Western blot images showing OAS1 band
A01684-3 · OAS1
A01684 · OAS1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for OAS1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for OAS1 in Western blot, you can use adipose tissue, which has high HPA expression.
Positive control: Adipose tissue (IHC candidate; verify WB)
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: No HPA tissue is listed as not detected, so use siRNA knockdown or a KO line for a clean negative control.
HPA tissue expression evidence for OAS1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | High | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | High | Protein (IHC) | HPA → |
| Appendix | glandular cells | High | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | High | Protein (IHC) | HPA → |
| Breast | glandular cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Smooth muscle | smooth muscle cells | Low | Protein (IHC) | HPA → |
| Heart muscle | cardiomyocytes | Medium | Protein (IHC) | HPA → |
| Lymph node | germinal center cells | Medium | Protein (IHC) | HPA → |
| Oral mucosa | squamous epithelial cells | Medium | Protein (IHC) | HPA → |
| Ovary | follicle cells | Medium | Protein (IHC) | HPA → |
Section 3
Advanced OAS1 Western Blot Tips
Deeper troubleshooting and optimisation questions for OAS1, answered from its protein features.
Why might an OAS1 band appear near 105 kDa?
Band shift · The supplied predicted mass is 46 kDa, while the reported apparent band is about 105 kDa. UniProt lists monomeric and homotetrameric OAS1, but those features do not establish the identity of a 105 kDa band. Verify it with an OAS1-specific control before interpreting the difference.
Which OAS1 isoforms could produce different bands?
Isoforms · UniProt lists p46, p42, p48 and p44. Their alternative sequences differ at the C terminus, so consider isoform expression when interpreting bands near their expected sizes. These sequence features alone do not explain a 105 kDa band.
Check whether the epitope overlaps canonical UniProt residues 347–400. This region is replaced in p48; p42 replaces 347–364 and lacks 365–400; p44 replaces 347–360 and lacks 361–400. These are UniProt canonical-sequence coordinates, which may differ from antibody or paper numbering.
Check whether the epitope overlaps canonical UniProt residues 347–400. This region is replaced in p48; p42 replaces 347–364 and lacks 365–400; p44 replaces 347–360 and lacks 361–400. These are UniProt canonical-sequence coordinates, which may differ from antibody or paper numbering.
Can a listed modification explain an OAS1 band shift?
PTM · Prenylation and lipoprotein appear among the UniProt keywords, but the supplied features give no modified-residue coordinates or glycosylation sites. Those labels do not demonstrate a visible shift or account for the difference between 46 and about 105 kDa.
Does this guide establish induction of OAS1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for OAS1?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A01684-3 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should multiple OAS1 bands be quantified?
Quantitation · Decide which bands are verified as OAS1 before combining their signals. UniProt lists four isoforms with distinct C-terminal sequences, so an antibody may detect them differently depending on its epitope. Report the band or bands measured and use the same choice across samples.
Does OAS1 oligomerization explain an unexpected band?
Interpretation · UniProt reports both monomeric and homotetrameric OAS1. That makes oligomerization relevant to investigate, but it does not establish that an unexpected Western-blot band is an oligomer. Confirm the band's identity before assigning it to OAS1.
UniProt lists OAS1 in the cytoplasm, mitochondrion, nucleus, microsomes and endoplasmic reticulum. When comparing fractions, account for where OAS1 may be present; a weak signal in one fraction need not mean low total OAS1.
UniProt lists OAS1 in the cytoplasm, mitochondrion, nucleus, microsomes and endoplasmic reticulum. When comparing fractions, account for where OAS1 may be present; a weak signal in one fraction need not mean low total OAS1.
Boster reagents
OAS1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-OAS1/3 antibody
Cat # A01684-3
Real WB data
Anti-OAS1 Antibody
Cat # A01684
Two antibodies catalogued for OAS1 list human and mouse reactivity and have Western blot images. A01684-3 shows HeLa and A549 lysates, but its reported band is approximately 105 kDa versus an expected 46 kDa. A01684 shows mouse liver cell extracts.
Which to pick: For human HeLa or A549 lysates, A01684-3 has the matching image, though its unexpected band size needs scrutiny. For mouse liver cell extracts, A01684 has the matching image. Both list human and mouse reactivity; these examples do not establish performance in every sample.
Source: BosterBio OAS1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P00973.
- Human Protein Atlas. OAS1 tissue expression.
- PMC7612834 — target-verified WB comparison
- PMC10511872 — target-verified WB comparison
- PMC11437775 — target-verified WB comparison