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- Table of Contents
OAS2 / 2'-5'-oligoadenylate synthase 2 · Western blot design guide
Design a Western Blot for OAS2
Real validated OAS2 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-OAS2 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
OAS2 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~82.4 kDa | |
| Gel | 8–10% (standard starting point) | |
| Positive control | Appendix (IHC candidate; verify WB) +4 more | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Acetylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 3 isoform(s) |
Section 1
Real Curated OAS2 Western Blot Protocols
The A01878 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | HeLa cells (catalog A01878) |
| Gel % | 8–10% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A01878; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | 1:20000 (catalog A01878) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected OAS2 Western Blot Band Size?
OAS2 has a predicted 82.4 kDa monomer; homodimerization and splice isoforms could affect bands, but their migration has not been demonstrated here.
| Band near 82.4 kDa | consistent with the predicted OAS2 monomer size |
| Band near 165 kDa | could reflect a homodimer if it survives sample preparation and electrophoresis |
| Several bands with different mobility | could include splice isoforms p71, p69, and 3; their separation is unverified |
| Band in cell lysate but little signal in medium | consistent with cytoplasmic localization |
💡Expected OAS2 appearanceUniProt predicts an 82.4 kDa OAS2 monomer; no empirical band size is supplied, and any higher or multiple bands need identity controls before assignment.
| Predicted monomer mass | provides an 82.4 kDa reference size |
| Homodimer formation | could yield a band near twice the monomer size if the complex survives electrophoresis |
| p71 splice isoform | may differ in size from other isoforms; its migration is unspecified |
| p69 splice isoform | may differ in size from other isoforms; its migration is unspecified |
| Isoform 3 | may differ in size from other isoforms; its migration is unspecified |
| Situation | Likely cause | Next action |
|---|---|---|
| Band higher than expected | a surviving OAS2 homodimer is possible | compare denaturing preparations and verify the band with an independent OAS2 antibody |
| Band lower than expected | a splice isoform or protein breakdown is possible | compare isoform expression and repeat with protease inhibitors |
| Multiple bands | OAS2 splice isoforms may contribute, but their migration is unknown | check band identity with an independent antibody or OAS2 depletion |
| Weak or no signal | little detectable OAS2 in the sample is possible | check sample loading and include a verified OAS2-positive lysate |
| Fragments below expected size | protein breakdown during handling is possible | prepare fresh lysate with protease inhibitors and compare antibody epitopes |
Experiment evidence
Western blot images showing OAS2 band
A01878 · OAS2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for OAS2 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for OAS2 in Western blot, you can use appendix tissue, which has high HPA expression.
Positive control: Appendix (IHC candidate; verify WB)
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside.
⚠️Feasibility: No supplied tissue is undetected, so use siRNA knockdown or a KO line as the negative control.
HPA tissue expression evidence for OAS2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Appendix | glandular cells | High | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | High | Protein (IHC) | HPA → |
| Caudate | glial cells | High | Protein (IHC) | HPA → |
| Cerebellum | cells in granular layer | High | Protein (IHC) | HPA → |
| Cerebral cortex | endothelial cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Medium | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Medium | Protein (IHC) | HPA → |
| Breast | adipocytes | Medium | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | Medium | Protein (IHC) | HPA → |
| Lung | alveolar cells | Medium | Protein (IHC) | HPA → |
Section 3
Advanced OAS2 Western Blot Tips
Deeper troubleshooting and optimisation questions for OAS2, answered from its protein features.
How should OAS2 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Which OAS2 isoforms could produce different bands?
Isoforms · UniProt lists p71, p69, and isoform 3. Compared with the canonical sequence, p69 changes residues 684–687 and lacks 688–719. Isoform 3 changes 151–172 and lacks 173–719. An antibody’s recognized sequence determines which isoforms it can detect; the isoform names alone do not establish band positions.
Can acetylation explain an OAS2 band shift?
PTM · UniProt lists N6-acetyllysine at canonical residue 378. This site is present in p69 but absent from isoform 3 because residues 173–719 are missing. Its annotation alone does not establish a visible mobility shift or explain a difference from predicted mass.
Does this guide establish induction of OAS2?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for OAS2?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A01878 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
What should OAS2 quantitation account for?
Quantitation · Decide whether the measurement is intended to represent one isoform or all detectable OAS2. The three annotated isoforms differ substantially in sequence, so antibody recognition may differ. Quantify the same defined band or set of bands across samples.
Should OAS2 run at its predicted 82.4 kDa?
Interpretation · 82.4 kDa is the predicted mass of the 719-residue canonical sequence. No observed band size is supplied, so use it as a reference rather than an expected exact position.
Consider the annotated splice variants alongside antibody recognition. p69 lacks canonical residues 688–719; isoform 3 lacks 173–719 and has a replacement at 151–172. A smaller band by itself cannot identify either isoform.
OAS2 is annotated as a homodimer, so dimerization is a possibility when investigating a higher band. That annotation does not establish that a dimer remains intact during Western blot preparation or identify the band.
UniProt places OAS2 in the cytoplasm and perinuclear region. These annotations support examining cytoplasmic material when planning sample preparation; they do not establish that OAS2 is restricted to a single fraction.
Consider the annotated splice variants alongside antibody recognition. p69 lacks canonical residues 688–719; isoform 3 lacks 173–719 and has a replacement at 151–172. A smaller band by itself cannot identify either isoform.
OAS2 is annotated as a homodimer, so dimerization is a possibility when investigating a higher band. That annotation does not establish that a dimer remains intact during Western blot preparation or identify the band.
UniProt places OAS2 in the cytoplasm and perinuclear region. These annotations support examining cytoplasmic material when planning sample preparation; they do not establish that OAS2 is restricted to a single fraction.
Boster reagents
OAS2 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-OAS2 Antibody
Cat # A01878
The catalog reports A01878, an anti-OAS2 antibody with reported human, mouse, and rat reactivity. Its Western blot image shows HeLa cells, with secondary antibody diluted 1:20,000; the supplied evidence does not show mouse or rat blots.
Which to pick: A01878 is the only listed option. Choose it for the documented HeLa Western blot context; human, mouse, and rat reactivity is listed, but the supplied image shows only HeLa cells.
Source: BosterBio OAS2 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P29728.
- Human Protein Atlas. OAS2 tissue expression.
- PMC13372824 — target-verified WB comparison
- PMC9851840 — target-verified WB comparison
- PMC6733796 — target-verified WB comparison