This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
OMP / Olfactory marker protein · Western blot design guide
Design a Western Blot for OMP
Real validated OMP Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-OMP WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
OMP Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~18.9 kDa | |
| Observed band | Approximately 17 kDa | |
| Gel | 5–20% (catalog A01781-1) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Acetylated | |
| Caveat | Band identity controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Real Curated OMP Western Blot Protocols
The A01781-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human Hela, human U251, human SH-SY5Y (catalog A01781-1) |
| Gel % | 5–20% (catalog A01781-1) |
| Load | 30 ug; reducing conditions (catalog A01781-1) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A01781-1) |
| Membrane | nitrocellulose membrane (catalog A01781-1) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A01781-1) |
| Primary antibody | A01781-1 · 0.5 μg/mL (catalog A01781-1) |
| Primary incubation | overnight at 4°C (catalog A01781-1) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A01781-1) |
| Secondary incubation | 1.5 hour at RT (catalog A01781-1) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A01781-1) |
| Detection | ECL (catalog A01781-1) |
Section 2
What Is the Expected OMP Western Blot Band Size?
OMP is predicted at 18.9 kDa and observed near 17 kDa; the cause of the difference is not established.
| Single band near 17 kDa | Matches the empirical OMP band reported in antibody QC |
| Single band near 19 kDa | Near the predicted 18.9 kDa mass of OMP |
| One sharp band | Consistent with the single annotated OMP isoform |
| No distinct acetylation-related band | N-acetylalanine at residue 2 does not establish a resolvable shift |
💡Expected OMP appearanceOMP has a predicted mass of 18.9 kDa, while antibody QC reports a band at approximately 17 kDa; the cause of the difference is unestablished, so verify identity with appropriate controls.
| Predicted molecular mass | Full-length OMP is predicted to be 18.9 kDa |
| 163-residue sequence | Defines the full-length protein used for the mass prediction |
| N-acetylalanine at residue 2 | A documented modification with no demonstrated visible band shift |
| Single annotated isoform | Provides no documented isoform size differences to explain additional bands |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | OMP may be below detection in the tested lysate | Check loading and antibody performance with an OMP-positive control |
| Band higher than expected | Its identity is unestablished; no glycosylation or disulfide feature explains it | Compare with an OMP-positive control and verify antibody specificity |
| Band lower than expected | The reported 17 kDa band runs below the 18.9 kDa prediction for an unestablished reason | Compare with the reported band and confirm identity using an independent antibody |
| Multiple bands | The single annotated isoform does not establish additional OMP bands | Check specificity with an OMP-negative control |
| Weak or no signal | OMP may be below the assay's detection limit | Check loading, transfer, and detection with an OMP-positive control |
| Fragments below expected size | Smaller bands have no assigned OMP fragment in the supplied features | Check sample integrity and test band identity with an independent antibody |
Experiment evidence
Western blot images showing OMP band
A01781-1 · OMP
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for OMP Western blot
🧪For positive controls for OMP in Western blot, you can use a positive tissue or cell sample once HPA data identify one.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA provides no expression data, so tissue-based positive and negative controls cannot be verified.
HPA tissue expression evidence for OMP
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced OMP Western Blot Tips
Deeper troubleshooting and optimisation questions for OMP, answered from its protein features.
How should OMP band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Should multiple OMP isoforms produce distinct bands?
Isoforms · UniProt lists one isoform and no alternative sequence. Its features therefore provide no basis for assigning separate bands to OMP isoforms.
How should OMP acetylation affect band interpretation?
PTM · UniProt identifies N-acetylalanine at position 2. Use that UniProt coordinate when comparing antibody or literature numbering. The listed modification does not, by itself, predict a resolvable Western blot shift.
UniProt lists no glycosylation sites and no signal peptide. Its features do not support assigning a higher band to glycosylated OMP; verify the band's identity instead.
UniProt lists no glycosylation sites and no signal peptide. Its features do not support assigning a higher band to glycosylated OMP; verify the band's identity instead.
Does this guide establish induction of OMP?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for OMP Western blot?
Transfer · OMP is predicted at 18.9 kDa and appears near 17 kDa in the supplied blots. Choose transfer conditions that retain proteins in this size range, and check membrane retention. UniProt features do not specify a particular transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A01781-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should OMP bands be quantified?
Quantitation · Quantify the identified OMP band near the reported 17 kDa position consistently across lanes, with sample normalization. Because UniProt lists one isoform, verify other bands before including them in the OMP measurement.
Why might OMP appear near 17 kDa rather than 18.9 kDa?
Interpretation · The supplied blots place OMP near 17 kDa, versus UniProt's predicted 18.9 kDa. UniProt lists N-acetylalanine at position 2, but that feature alone does not explain the difference or establish a visible shift. Confirm the band's identity before attributing the discrepancy to modification.
UniProt reports interactions with BEX1 and BEX2, but this does not identify additional Western blot bands. Its single listed isoform also provides no basis for labeling extra bands as isoforms. Confirm unexpected bands independently.
UniProt reports interactions with BEX1 and BEX2, but this does not identify additional Western blot bands. Its single listed isoform also provides no basis for labeling extra bands as isoforms. Confirm unexpected bands independently.
Boster reagents
OMP Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-OMP Antibody Picoband®
Cat # A01781-1
The catalog reports one anti-OMP antibody, A01781-1, for Western blotting. Its WB image shows human HeLa, U251, and SH-SY5Y lysates and rat PC-12 lysate, with a band near 17 kDa versus the expected 19 kDa. No mouse WB sample is shown.
Which to pick: A01781-1 is the only listed option. It is listed as reactive with human, mouse, and rat; the supplied WB image documents the specified human and rat lysates. For mouse samples, the supplied image provides no direct example.
Source: BosterBio OMP gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P47874.
- Human Protein Atlas. OMP tissue expression.
- PMC5938008 — target-verified WB comparison