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- Table of Contents
Facts about Hepatocyte nuclear factor 6.
Important for liver genes transcription. .
Human | |
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Gene Name: | ONECUT1 |
Uniprot: | Q9UBC0 |
Entrez: | 3175 |
Belongs to: |
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CUT homeobox family |
HNF6; HNF-6; HNF-6A; HNF6Ahepatocyte nuclear factor 6, alpha; HNF6hepatocyte nuclear factor 6; HNF-6One cut domain family member 1; OC-1; one cut homeobox 1one cut domain, family member 1; ONECUT1
Mass (kDA):
51.023 kDA
Human | |
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Location: | 15q21.3 |
Sequence: | 15; NC_000015.10 (52755053..52790336, complement) |
Highly expressed in liver; lower expression in testis and skin.
Nucleus.
If you are interested in discovering what the ONECUT1 marker is and how it can benefit your studies, read on. Listed below are some of the benefits and applications of this marker. This marker can be used in order to analyze HNF6's effects on the liver. HNF6, also known as Hepatosplenic nuclear factor, is a vital gene that regulates activity in various organs.
The ONECUT1 gene is part of a tightly regulated network that regulates genes involved in endocrine, pancreatic development. Several of these genes are involved in multiple forms of diabetes, including monogenic and multifactorial forms. Using the human stem cell differentiation model, scientists are able to identify genes involved in the development of diabetes. ONECUT1 has been associated with the growth of pancreatic tissues, which may explain its involvement with pancreatic disorders.
ONECUT1 binds actively to pancreatic bcells' enhancers. It may also play a role when these cells are primed for endocrine-related differentiation. Nkx6.1, a key genes involved in pancreatic cells development, is closely linked to the ONECUT1 protein. The ONECUT1 gene is also associated with a pancreatic b-cell-specific program, wherein ONECUT1-bound pancreatic enhancers accumulate H3K4me1 during the GT stage and H3K27ac from the GT stage to PP.
The ONECUT1 Gene is associated not only with chromatin dynamics but also with a variety t-regulatory elements. This gene binds multiple genes, including FOXA (PDX1), GATA6 (GAT6), and FOXA (FOXA). Researchers can identify the most critical regulatory and transcriptional elements that impact chromatin dynamics by studying the activity of trans-regulatory elements.
This gene is found in mouse retinas as bi-polar. Its differential expression may indicate the existence of two distinct gene regulator networks. Onecut1 is also known to bind multiple TFs, suggesting the existence distinct gene-regulatory network. And it is known to be an important regulator of gene-regulatory pathways. RNA-seq clusters reflect its corresponding gene expression.
ONECUT1 (a genetically variable gene that identifies type I diabetes) is a marker. Boster Bio's ONECUT1 ELISA tests have a high sensitivity that allows for detection of ONECUT1 with samples as small and as low as 10 ng. Moeller A.M., along with colleagues, identified associations between genetic variability (genetic variation) and type II Diabetes in a recent study.
The ONECUT1 protein, also known by hepatocyte nuclear factors 6, is a monomeric, 53 to 56 kDa protein mapped at 15q21.3. It is involved in the regulation of glucose metabolism and cell cycle. The gene may be related to cancer. ONECUT1 has no known targets at this time, but it could be used in genetic studies.
Four TF genes are responsible for regulating the ONECUT1 protein, which includes AFP. Most genes tested encode for AFP transcription repressors FOS, HNF4, JUN1, and ZBTB20. Downregulation of these genes shows an overall gene network alteration. Adult liver cells normally suppress AFP expression. Boster Bio's OneCUT1 reference manual provides more information.
Trans-regulatory elements were introduced into the developing retinas of mice and chickens to aid in understanding the functions ONECUT1 plays in the retina. After trans-regulatory components were introduced, reporter activity was characterized and correlated against cell type markers. To determine the downstream elements, high-throughput flow Cytometry assays were created. These assays were validated and the benefits of ONECUT1 became apparent.
Onecut1 encodes one gene that encodes a transcription molecule that represses Nrl/L-Maf and promotes the expression of cone-associated genes. The gene may play a pivotal role in determining cell fate. A dominant-negative ONECUT1 construct results in qualitative inductions of L-Maf, and the cow-rhodopsin elements in the retina. The CAG promoter drives Onecut1's DNA binding domain.
The ONECUT1 gene is expressed in the pancreas, liver and cerebellum. The ONECUT1 marker can be used for many purposes, including the diagnosis of diabetes or cancer. The gene is found in all these tissues and is present in many other tissues, including the lung, intestines, and endocrine glands. The precise function of ONECUT1's gene is unknown.
Among the many functions of the ONECUT1 gen, it has been used to identify the type of cell that carries the variant. In the case with a gene edited HUES8 HESC, ONECUT1 nil cells showed distinct differentiation pathways. Null cultures did not have certain gene programs that could be used to identify endocrine cells. Quantitative proteomics showed that ONECUT1 cells were less likely to be WT than their null counterparts.
The ONECUT1 family is composed three major subunits. Each of these regulates the expression downstream genes. There are many functional variants in the ONECUT1 gene, some of which can affect downstream factors. ONECUT1 variants located in the promoter area were associated with multifactorial NKX gene activation and T2D. Although many applications of the gene are currently under investigation, ONECUT1 is a crucial factor in pancreatic physiology.
In a recent study, ONECUT1 mutations were rare in the German population. Sequencing of the gene has been performed on the entire genome of diabetic patients. The Supplementary Table includes the sequences of the ONECUT1 enons. It includes primer sequences that correspond to the ONECUT1 Gene. The data collected in this study will provide additional information that will aid researchers in understanding the pathophysiology of diabetes.
PMID: 10491763 by Moeller A.M., et al. Hepatocyte nuclear factor-6: associations between genetic variability and type II diabetes and between genetic variability and estimates of insulin secretion.