|Validated Species:||Human, Mouse, Rat|
|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-HNF6 Antibody|
|Description||Rabbit IgG polyclonal antibody for Hepatocyte nuclear factor 6(ONECUT1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-HNF6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2214)|
|Replacement Item||This antibody may replace the following items: sc-13050|sc-13050-X|sc-376167|sc-376167-X|sc-376308|sc-376308-X|sc-6559|sc-6559-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human HNF6(187-203aa LGSIHNSQQGLPHYAHP), identical to the related mouse and rat sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Hepatocyte nuclear factor 6|
|Molecular Weight||51023 MW|
|Protein Function||Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.|
|Tissue Specificity||Highly expressed in liver; lower expression in testis and skin.|
|Sequence Similarities||Belongs to the CUT homeobox family.|
|Alternative Names||Hepatocyte nuclear factor 6;HNF-6;One cut domain family member 1;One cut homeobox 1;ONECUT1;HNF6, HNF6A;|
|Research Areas|||signal transduction|metabolism|energy metabolism| epigenetics and nuclear signaling|transcription|other factors|transcription factors| developmental biology|lineage specification|endoderm|organogenesis|gut development|liver development|pancreas development| metabolism|pathways and processes|metabolic signaling pathways|energy transfer pathways|types of disease|cancer||
Background for Hepatocyte nuclear factor 6
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-HNF6 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-HNF6 Antibody Images
Click the images to enlarge.
Lane 1: Rat Liver Tissue Lysate
Lane 2: Mouse Liver Tissue Lysate
Lane 3: HELA Cell Lysate
IHC(P): Human Liver Cancer Tissue
IHC(P): Rat Liver Tissue
IHC(F): Rat Liver Tissue
ICC: HCT116 Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,