This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
OVGP1 / Oviduct-specific glycoprotein · IHC design guide
Design Immunohistochemistry for OVGP1
Plan OVGP1 IHC-P using fallopian tube non-ciliated cells as the positive reference (HPA tissue IHC). The catalog antibody has a documented formaldehyde-fixed paraffin workflow at 1:25 primary dilution (datasheet M07911); account for secretion when interpreting tissue staining (UniProt).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
OVGP1 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Secretory vesicles expected (UniProt); tissue compartment unassigned (HPA tissue IHC) | |
| Staining pattern | High in fallopian tube non-ciliated cells; compartment unspecified (HPA tissue IHC) | |
| Antigen retrieval | Citrate pH 6 HIER, heat-mediated (datasheet M07911) | |
| Positive control | Fallopian tube | |
| Negative control | Adipose tissue+4 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Formaldehyde-fixed paraffin sections (selected-SKU IHC image M07911) | |
| Caveat | Secreted protein may stain away from its RNA site (HPA tissue IHC) | |
| Regulation | Estrogen-dependent (UniProt) | |
| Isoform / epitope | No isoforms; signal peptide 1–21 cleaved, no cytoplasmic tail (UniProt) |
Section 1
Recommended OVGP1 IHC & IF Protocols
The catalog antibody’s IHC-P protocol is accompanied by four published OVGP1 IHC protocols (PMC7457448; PMC7661263; PMC11954873; PMC11447938).
| Sample | Formaldehyde-fixed, paraffin-embedded human fallopian tube tissue (datasheet M07911) |
| Fixation | Image formalin-fixed; duration unreported (datasheet M07911); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: Citrate pH 6 (datasheet M07911); 20 min, 95–100 °C (standard) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% normal serum of the secondary host, 30 min, room temperature (standard) |
| Primary antibody | Rabbit anti-OVGP1, 1:25 (datasheet M07911) |
| Primary incubation | 1 hours at 37°C (datasheet M07911) |
| Detection | HRP-polymer secondary, DAB chromogen 5–10 min (standard) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | OVGP1-positive staining in non-ciliated cells of fallopian tube (HPA tissue IHC: High). HPA tissue profile: Selective expression in fallopian tube. No signal in the no-primary control. |
💡Decision noteStart with citrate pH 6 heat retrieval (datasheet M07911); the bovine IHC protocol also specifies citrate pH 6 (PMC11447938).
Section 2
What Is the Expected OVGP1 Staining Pattern?
OVGP1 is a secretory-vesicle protein with no annotated transmembrane segment (UniProt Q12889 subcellular location; topology). In paraffin-section IHC, expect strong staining in fallopian-tube non-ciliated cells (HPA: High in non-ciliated cells; tissue IHC reliability: Enhanced). As a secreted protein, its staining location need not exactly match the cells with OVGP1 RNA (HPA tissue IHC reliability description).
| Strong staining in fallopian-tube non-ciliated cells, with a cytoplasmic, vesicle-associated appearance (HPA: High in non-ciliated cells; UniProt Q12889 subcellular location). | This fits the reported cell-type pattern and secretory-vesicle annotation (HPA tissue IHC; UniProt Q12889). Judge the cell type and compartment together; staining intensity alone does not establish specificity (general IHC practice). |
| Predominantly nuclear or sharp plasma-membrane staining in non-ciliated cells (UniProt Q12889 subcellular location; topology). | Those compartments do not match the annotated secretory vesicles or the absence of a transmembrane segment (UniProt Q12889). Review controls and antibody specificity before calling this OVGP1 (general IHC practice). |
| Strong staining in adipocytes or adrenal glandular cells (HPA: Not detected in these sampled cell types). | This conflicts with the reported tissue IHC pattern (HPA tissue IHC). Consider cross-reactivity or endogenous detection activity; an HPA ‘Not detected’ result is a reference observation, not proof that every specimen must be negative (HPA tissue IHC; general IHC practice). |
| Diffuse staining across tissue compartments, without a discernible non-ciliated-cell pattern (HPA: High in fallopian-tube non-ciliated cells). | A broadly distributed signal is difficult to assign to the reported positive cells (HPA tissue IHC). Check background in a control lacking primary antibody and reassess blocking, detection and washes (general IHC practice). |
| No detectable signal in a fallopian-tube section expected to contain non-ciliated cells (HPA: High in non-ciliated cells). | First confirm that the expected cells are present and the tissue and detection controls worked (general IHC practice). Then review the antibody’s IHC validation and the chosen retrieval and detection conditions; this result alone does not establish absent OVGP1 (general IHC practice). |
💡Expected OVGP1 appearanceCall a positive result when fallopian-tube non-ciliated cells show strong, cytoplasmic vesicle-associated staining (HPA: High in non-ciliated cells; UniProt Q12889); dominant nuclear or membrane staining is discordant (UniProt Q12889 subcellular location; topology).
| Tissue and cell selection (HPA tissue IHC). | Fallopian tube supplies the reported positive pattern, specifically high staining in non-ciliated cells (HPA tissue IHC). HPA reports no detection in several sampled cell types, including adipocytes and adrenal glandular cells (HPA tissue IHC). |
| Secretory location and topology (UniProt Q12889). | OVGP1 is annotated in secretory vesicles and lacks a transmembrane segment (UniProt Q12889). Interpret intracellular staining in that context; HPA cautions that RNA and protein tissue locations can differ for secreted proteins (HPA tissue IHC). |
| Protein processing and glycosylation (UniProt Q12889). | The annotated signal peptide spans residues 1–21 and the mature chain spans 22–678; five glycosylation sites are listed (UniProt Q12889). These annotations do not identify an antibody epitope or establish a target-specific retrieval requirement (UniProt Q12889; evidence scope). |
| IHC antibody evidence (HPA antibody validation). | HPA062205 has Enhanced IHC status, while HPA031666 has no listed IHC status in the supplied record (HPA antibody validation). Apply that distinction only to those antibodies; it does not validate an unspecified catalog antibody (HPA antibody validation). |
| IF/ICC Q: What location is supported? (HPA subcellular ICC-IF). | A: Vesicles are the supported main location; the additional microtubule location is uncertain (HPA subcellular ICC-IF). HPA031666 has Supported ICC status (HPA antibody validation). Use the separate IF/ICC guide for that application. |
| Situation | Likely cause | Next action |
|---|---|---|
| Fallopian-tube non-ciliated cells are unstained (HPA: High in non-ciliated cells). | The expected cells may be absent from the section, or the IHC workflow may have failed (general IHC practice). | Confirm cell identity and section quality, then check positive and detection controls and the antibody’s IHC instructions (general IHC practice). |
| Nuclei dominate the signal (UniProt Q12889: secretory vesicles). | A dominant nuclear pattern is discordant with the annotated location; its source is unresolved from this slide alone (UniProt Q12889; general IHC practice). | Compare with a control lacking primary antibody and reassess specificity before scoring nuclear signal as OVGP1 (general IHC practice). |
| Adipocytes or adrenal glandular cells stain strongly (HPA: Not detected). | The signal conflicts with HPA’s sampled-cell observations; cross-reactivity or endogenous detection activity are possibilities (HPA tissue IHC; general IHC practice). | Inspect controls lacking primary antibody and review blocking and detection reagents; compare the fallopian-tube pattern (general IHC practice; HPA tissue IHC). |
| Diffuse colour obscures cell boundaries (general IHC practice). | Background from detection or insufficient washing can obscure cell-specific interpretation (general IHC practice). | Check the control lacking primary antibody, blocking and washes; score only interpretable staining in the expected cells (general IHC practice; HPA tissue IHC). |
| Only the luminal or extracellular space appears positive (UniProt Q12889: secretory protein). | Secreted protein may be separated from the cells that express its RNA (HPA tissue IHC reliability description). Extracellular signal alone cannot identify the producing cell (general IHC practice). | Examine whether non-ciliated cells also show the reported high staining, and interpret extracellular signal with tissue morphology and controls (HPA tissue IHC; general IHC practice). |
| An IF/ICC image shows apparent microtubule staining (HPA subcellular ICC-IF). | HPA labels microtubules an uncertain additional location; vesicles are the supported main location (HPA subcellular ICC-IF). | Treat microtubule signal as provisional and consult the separate IF/ICC guide; do not use it to redefine the paraffin-section IHC pattern (HPA subcellular ICC-IF; HPA tissue IHC). |
Experiment evidence
IHC & IF images showing OVGP1 staining
M07911 · IHCM07911 staining OVGP1 in human fallopian tube tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sect
A07911 · IFImmunocytochemistry of OVGP1 in 293 cells with OVGP1L antibody at 2.5 μg/mL.
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000085465-OVGP1/tissue/cerebral+cortex#imid_17655531
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000085465-OVGP1/tissue/colon#imid_17655441
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000085465-OVGP1/tissue/liver#imid_17655442
HPA · KidneyHuman Protein Atlas IHC, Kidney. Source: https://www.proteinatlas.org/ENSG00000085465-OVGP1/tissue/kidney#imid_17655451
HPA · ICC-IF HEK293Human Protein Atlas immunofluorescence, HEK293, antibody HPA031666; green = OVGP1, blue = nucleus, red = microtubules.
HPA · ICC-IF HEK293Human Protein Atlas immunofluorescence, HEK293, antibody HPA031666; green = OVGP1, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for OVGP1 IHC & IF
🧪Run fallopian tube first and look for staining in its non-ciliated cells (HPA: High in fallopian tube non-ciliated cells). Use adipose tissue as a negative comparator (HPA: Not detected in adipocytes); cells outside the positive compartment on the fallopian tube slide should show only background-level staining.
Positive control tissue: Fallopian tube (Non-ciliated cells, HPA High)
Negative control tissue: Adipose tissue (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show OVGP1 in HEK293, RT-4, U2OS, with annotated localisation: Vesicles (supported) (HPA subcellular).
Technical controls: Include a no-primary, secondary-only control; an isotype control matched to the primary antibody’s host species and clonality; and, if available, OVGP1-knockout material or a validated immunizing-peptide block. For chromogenic detection, quench endogenous peroxidase and assess endogenous biotin when using the caption’s biotinylated secondary antibody (selected-SKU tissue-IHC caption: biotinylated secondary antibody).
⚠️Feasibility: A target-specific fixation window or fixation effect is unreported, and the selected-SKU tissue-IHC caption’s fixative field is unreported. The caption uses heat-mediated citrate retrieval at pH 6, but does not establish that retrieval is required (selected-SKU tissue-IHC caption: citrate retrieval, pH 6); the supplied evidence does not establish whether frozen sections or IF are easier. OVGP1 is associated with secretory vesicles (UniProt Q12889: secretory vesicle), so assess luminal staining separately from staining within non-ciliated cells (HPA: High in fallopian tube non-ciliated cells).
HPA tissue IHC evidence for OVGP1
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Enhanced — High consistency between antibody staining and RNA expression data. Secreted protein, tissue location of RNA and protein is expected to differ.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Fallopian tube | Non-ciliated cells | High | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | Adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | Glandular cells | Not detected | Protein (IHC) | HPA → |
| Appendix | Glandular cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | Hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Breast | Adipocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced OVGP1 IHC Tips
Use fallopian tube non-ciliated cells and a matched negative tissue to assess OVGP1 staining (HPA tissue IHC).
Which retrieval conditions should I start with for OVGP1 paraffin sections?
Start with heat-mediated citrate retrieval at pH 6 for OVGP1 paraffin sections (datasheet M07911). The selected antibody was shown staining human fallopian tube after this retrieval, but its caption gives no heating duration or temperature (M07911 tissue-IHC caption). Keep retrieval conditions identical across comparison sections, then optimize heating time in small increments if staining is weak while checking tissue morphology (standard IHC practice). If signal remains weak, test another retrieval buffer or pH as a fallback on adjacent sections, retaining the citrate condition as the reference and assessing non-ciliated cells against a negative tissue (HPA tissue IHC; standard IHC practice).
How should I assess whether fixation is suppressing OVGP1 staining?
Target-specific sensitivity to fixation is unknown from the supplied evidence; do not infer it from OVGP1 localisation or glycosylation (UniProt Q12889; supplied fixation rule). Record fixative, fixation duration, processing history, and section age for each paraffin block before comparing stain intensity (standard IHC practice). If matched specimens are available, compare shorter and longer fixation conditions using the same citrate retrieval at pH 6 and identical antibody and detection settings (datasheet M07911; standard IHC practice). Judge the comparison in preserved non-ciliated epithelium, using tissue morphology and background staining alongside OVGP1 signal rather than intensity alone (HPA tissue IHC; standard IHC practice).
Where should convincing OVGP1 staining appear in a fallopian tube section?
Prioritize staining in fallopian tube non-ciliated cells, where tissue IHC reports high OVGP1 expression (HPA tissue IHC). OVGP1 is associated with secretory vesicles, and HPA supports vesicular localisation, so cytoplasmic granular staining is biologically plausible (UniProt Q12889 subcellular; HPA subcellular). Its signal peptide spans residues 1–21, and the protein has no transmembrane segment; luminal secreted material can therefore accompany cellular staining, but should be interpreted with the cellular pattern (UniProt Q12889 topology and processing; standard IHC interpretation). Compare epithelial staining with adjacent structures and an OVGP1-negative tissue under the same chromogenic settings before calling diffuse extracellular colour specific (HPA tissue IHC; standard IHC practice).
Could epitope location or glycosylation explain weak OVGP1 IHC?
The record lists no OVGP1 isoforms, so an isoform switch is unsupported as an explanation for discordant staining (UniProt Q12889 isoforms). Its signal peptide occupies residues 1–21, the mature chain begins at 22, and annotated glycosylation sites occur at 402, 441, 580, 596, and 648 (UniProt Q12889 processing and glycosylation). The supplied evidence does not map this antibody's epitope, so it cannot establish whether processing, glycosylation, or retrieval masks antibody binding (supplied record; datasheet M07911). If staining is weak, compare the prescribed citrate pH 6 condition with a carefully controlled retrieval fallback and seek epitope information before assigning a molecular cause (datasheet M07911; standard IHC practice).
How can I check an OVGP1 IHC pattern with multiplex immunofluorescence?
For a complementary IF check, pair OVGP1 with a validated marker of fallopian tube non-ciliated cells, the population reported as strongly positive in tissue IHC (HPA tissue IHC; standard IF practice). Choose spectrally separated fluorophores and place the weaker signal in a channel with less tissue autofluorescence, checking single-stain and unstained controls before interpreting overlap (standard IF practice). OVGP1 is associated with secretory vesicles and has no transmembrane segment, but this antibody's epitope side is not mapped (UniProt Q12889 subcellular and topology; supplied record). Test gentle permeabilisation if intracellular or vesicle-luminal access is required, and verify that it preserves morphology and the expected cellular pattern (standard IF practice; HPA tissue IHC).
What should I check when OVGP1 chromogenic staining looks diffuse?
Check the no-primary control for detection-system colour and inspect section edges, folds, and damaged areas before interpreting diffuse signal (standard IHC practice). The selected tissue-IHC caption reports 3% BSA blocking for 0.5 hour and a 1:25 primary incubation for 1 hour at 37°C; treat these as documented starting conditions for that antibody (M07911 tissue-IHC caption). For peroxidase-based detection, verify peroxide blocking and assess endogenous enzyme signal with appropriate controls (standard IHC practice). OVGP1 can be secreted, so evaluate extracellular colour alongside staining in fallopian tube non-ciliated cells and a matched negative tissue rather than treating every luminal deposit as background (UniProt Q12889 processing; HPA tissue IHC; standard IHC interpretation).
How should I score OVGP1 staining across paraffin sections? ⚠ ANSWER MARKED FOR VERIFICATION
Define the fallopian tube non-ciliated epithelial compartment before scoring, because that is the reported high-expression population (HPA tissue IHC). Record the percentage of positive cells and intensity on a consistent 0–3 scale, then calculate an H-score from 0–300 for that compartment (standard IHC practice). Normalize counts or stained area to the number of evaluable non-ciliated cells or their measured epithelial area, and report excluded folds, damaged tissue, and luminal deposits separately (standard IHC practice). Keep section processing, citrate retrieval at pH 6, imaging, and scoring thresholds consistent across the comparison set (datasheet M07911; standard IHC practice).
How do I distinguish true OVGP1 staining from artefact?
A credible positive shows staining in fallopian tube non-ciliated cells and may include a vesicular cytoplasmic pattern, consistent with tissue and subcellular evidence (HPA tissue IHC; HPA subcellular). OVGP1 has no transmembrane segment and is associated with secretory vesicles, so a crisp membrane-only pattern warrants further checking (UniProt Q12889 topology and subcellular; standard IHC interpretation). Compare suspicious colour with a no-primary control and an OVGP1-negative tissue, and inspect whether it tracks section edges, necrosis, or endogenous enzyme activity (HPA tissue IHC; standard IHC practice). Require the expected cellular pattern to persist across well-preserved fields before assigning a positive 0–3 intensity score (HPA tissue IHC; standard IHC practice).
Boster reagents
Best OVGP1 / Oviduct-specific glycoprotein IHC Antibodies
The catalog pairs human fallopian tube paraffin-section IHC data with 293-cell IF/ICC data (M07911 and A07911 figure captions). Listed reactivity covers human, mouse, and rat (catalog reactivity).
Real IHC data
Anti-OVGP1 Antibody (N-Term)
Cat # M07911
Real IF data
Anti-Oviduct-specific glycoprotein OVGP1 Antibody
Cat # A07911
M07911 is listed for human IHC-P and has an OVGP1 staining image from human fallopian tube paraffin sections at 1:25 (catalog applications and reactivity; M07911 figure caption). A07911 is listed for IF/ICC and has 293-cell images at 2.5 μg/mL; its listed reactivity is human, mouse, and rat (catalog applications and reactivity; A07911 figure captions).
Which to pick: Choose M07911 for human paraffin-section IHC: it is listed as polyclonal and IHC-P tested, with its own fallopian tube section image (M07911 dilution notes; catalog applications; M07911 figure caption). Choose A07911 for IF/ICC because those applications and 293-cell images are listed for it; the captions do not report cell fixation (catalog applications; A07911 figure captions). For mouse or rat work, A07911 is the listed option by reactivity, but its supplied IF/ICC images show 293 cells only (catalog reactivity; A07911 figure captions).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry Q12889 (OVGP1_HUMAN, Oviduct-specific glycoprotein).
- Human Protein Atlas. OVGP1 tissue IHC expression (reliability: Enhanced).
- Human Protein Atlas. OVGP1 subcellular location (ICC-IF): Mainly localized to vesicles. In addition localized to the microtubules..
- Human Protein Atlas. OVGP1 antibody validation summary (2 antibodies).
- Lineage tracing suggests that ovarian endosalpingiosis does not result from escape of oviductal epithelium. The Journal of pathology 2019 — PMC7457448.
- Comparative gene expression profiling of mouse ovaries upon stimulation with natural equine chorionic gonadotropin (N-eCG) and tethered recombinant-eCG (R-eCG). BMC biotechnology 2020 — PMC7661263.
- Spatial transcriptomic profiling of the human fallopian tube epithelium reveals region-specific gene expression patterns. Communications biology 2025 — PMC11954873.
- Maternal stress and the early embryonic microenvironment: investigating long-term cortisol effects on bovine oviductal epithelial cells using air-liquid interface culture. Journal of animal science and biotechnology 2024 — PMC11447938.
- PubMed PMID:7819450 — UniProt-cited evidence.
- PubMed PMID:16710414 — UniProt-cited evidence.
- PubMed PMID:15489334 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for OVGP1 (Oviduct-specific glycoprotein). Expected localisation Secretory vesicles expected (UniProt); tissue compartment unassigned (HPA tissue IHC). Antigen retrieval: Citrate pH 6 HIER, heat-mediated (datasheet M07911). Validated IHC antibody: M07911 at 1:25 (the product's own IHC validation image is shown). Controls (HPA): positive Fallopian tube (Non-ciliated cells); negative Adipose tissue. IHC-P workflow on paraffin sections. Protocol steps: 1. Sample: Formaldehyde-fixed, paraffin-embedded human fallopian tube tissue (datasheet M07911); 2. Fixation: Image formalin-fixed; duration unreported (datasheet M07911); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: Citrate pH 6 (datasheet M07911); 20 min, 95–100 °C (standard); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% normal serum of the secondary host, 30 min , room temperature (standard); 8. Primary antibody: Rabbit anti-OVGP1, 1:25 (datasheet M07911); 9. Primary incubation: 1 hours at 37°C (datasheet M07911). Watch for: Fixation: Formaldehyde-fixed paraffin sections (selected-SKU IHC image M07911); Caveat: Secreted protein may stain away from its RNA site (HPA tissue IHC); Isoform / epitope: No isoforms; signal peptide 1–21 cleaved, no cytoplasmic tail (UniProt); Regulation: Estrogen-dependent (UniProt). Evidence: UniProt Q12889, HPA tissue IHC · Enhanced, HPA ICC-IF · Mainly localized to vesicles. In addition localized to the…, Datasheet M07911, PMC7457448, PMC7661263. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/OVGP1/immunohistochemistry-ihc-guide.