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- Table of Contents
PAN2 / PAN2-PAN3 deadenylation complex catalytic subunit PAN2 · Western blot design guide
Design a Western Blot for PAN2
Source-linked PAN2 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-PAN2 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
PAN2 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~135.4 kDa | |
| Observed band | ~135 kDa | |
| Gel | 5–20% (catalog A03834-1) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 3 isoform(s) |
Section 1
Source-Linked PAN2 Western Blot Protocol Options
The A03834-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human HepG2, human Hel, human Daudi, rat PC-12, mouse thymus, mouse NIH/3T3 (catalog A03834-1) |
| Gel % | 5–20% (catalog A03834-1) |
| Load | 30 ug; reducing conditions (catalog A03834-1) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A03834-1) |
| Membrane | nitrocellulose membrane (catalog A03834-1) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A03834-1) |
| Primary antibody | A03834-1 · 0.25 μg/mL (catalog A03834-1) |
| Primary incubation | overnight at 4°C (catalog A03834-1) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A03834-1) |
| Secondary incubation | 1.5 hour at RT (catalog A03834-1) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A03834-1) |
| Detection | ECL (catalog A03834-1) |
Section 2
What Is the Expected PAN2 Western Blot Band Size?
PAN2 is predicted at 135.4 kDa and observed at approximately 135 kDa; the cause of the small difference is not established.
| Band at approximately 135 kDa in whole-cell lysate | Matches the empirical PAN2 band; confirm identity with appropriate controls |
| Band near 135 kDa in a cytoplasmic fraction | Consistent with cytoplasmic PAN2 |
| Band near 135 kDa in a nuclear fraction | Consistent with nuclear PAN2 |
| Additional bands at other positions | Could reflect PAN2 isoforms 1, 2, or 3, but distinct migration is unestablished |
💡Expected PAN2 appearancePAN2 has a predicted mass of 135.4 kDa and an observed band at approximately 135 kDa in whole-cell lysates; confirm band identity with appropriate controls.
| Predicted PAN2 mass | Places the reference band near 135.4 kDa |
| Splice isoform 1 | Its individual mass and migration relative to the other isoforms are unspecified |
| Splice isoform 2 | Its individual mass and migration relative to the other isoforms are unspecified |
| Splice isoform 3 | Its individual mass and migration relative to the other isoforms are unspecified |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | PAN2 signal may be below detection in the sampled lysate | Check loading and antibody performance against a positive whole-cell lysate |
| Band higher than expected | The band is unassigned; isoform migration differences are unestablished | Compare with the approximately 135 kDa control band and verify identity by PAN2 depletion |
| Band lower than expected | The band is unassigned; isoform migration differences are unestablished | Compare with the approximately 135 kDa control band and verify identity by PAN2 depletion |
| Multiple bands | PAN2 has isoforms 1, 2, and 3, but their separate band positions are unknown | Test which bands respond to PAN2 depletion and confirm isoforms independently |
| Weak or no signal | PAN2 distributes between cytoplasm and nucleus, so the sampled fraction may contain little target | Compare whole-cell, cytoplasmic, and nuclear preparations with loading controls |
Experiment evidence
Western blot images showing PAN2 band
A03834-1 · PAN2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PAN2 Western blot
🧪For positive controls for PAN2 in Western blot, you can use no HPA-supported tissue or cell sample because no expression data were supplied.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: PAN2 is intracellular, but the missing HPA data prevent selection of a tissue positive or negative control.
HPA tissue expression evidence for PAN2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced PAN2 Western Blot Tips
Deeper troubleshooting and optimisation questions for PAN2, answered from its protein features.
Does PAN3 association change the expected PAN2 band?
Band shift · PAN2 forms a complex with a PAN3 homodimer, but its listed predicted mass of 135.4 kDa is for PAN2 itself. Interpret the approximately 135 kDa band as consistent with PAN2; the complex description alone does not establish an additional PAN2 band.
Could PAN2 isoforms produce additional bands?
Isoforms · UniProt lists three isoforms. Relative to isoform 1, isoform 2 lacks residues 689–692 and isoform 3 lacks residue 643. These small deletions may be difficult to distinguish by size alone; check whether the antibody recognizes a region shared by the isoforms.
Can phosphorylation change PAN2 band migration?
PTM · UniProt lists phosphoserine at positions 791 and 1189, using UniProt sequence numbering. Their presence alone does not establish a visible band shift. If migration changes between samples, compare phosphatase-treated and untreated lysates to assess a phosphorylation contribution.
Does this guide establish induction of PAN2?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for PAN2 Western blot?
Transfer · PAN2 is approximately 135 kDa, so choose transfer conditions that recover proteins of that size. Check both membrane capture and protein remaining in the gel before interpreting a weak PAN2 signal.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A03834-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should PAN2 bands be quantified across samples?
Quantitation · Measure bands consistently near the expected 135 kDa region and normalize to an appropriate loading measure. PAN2 occurs in the cytoplasm and nucleus and can localize to P-bodies, so compare equivalent sample fractions when assessing changes in abundance.
Why is the PAN2 band near 135 kDa?
Interpretation · PAN2 has a predicted mass of 135.4 kDa, close to the reported apparent band at approximately 135 kDa. Its listed features do not establish a cause for any small difference between calculated and apparent mass.
Check their size against the approximately 135 kDa band and consider the three listed isoforms. The documented deletions are only one or four residues, so they do not by themselves explain a large size difference. The listed phosphorylation sites likewise do not establish a visible shift.
Check their size against the approximately 135 kDa band and consider the three listed isoforms. The documented deletions are only one or four residues, so they do not by themselves explain a large size difference. The listed phosphorylation sites likewise do not establish a visible shift.
Boster reagents
PAN2 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-PAN2 Antibody Picoband®
Cat # A03834-1
The catalog reports one anti-PAN2 antibody for Western blot, A03834-1. Its product image reports an approximately 135 kDa band in human cell, rat cell, and mouse cell and tissue lysates. No publication evidence was supplied.
Which to pick: A03834-1 is the only listed option. It reports Human, Mouse, and Rat reactivity and includes a Western blot image using specified lysates from each species; choose it when those reported test contexts suit your experiment.
Source: BosterBio PAN2 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q504Q3.
- Human Protein Atlas. PAN2 tissue expression.
- PMC5558910 — target-verified WB comparison
- PMC2447901 — target-verified WB comparison