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- Table of Contents
PBOV1 / Prostate and breast cancer overexpressed gene 1 protein · Western blot design guide
Design a Western Blot for PBOV1
Source-linked PBOV1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-PBOV1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled October 2026 · For research use; verify linked source records and product datasheet before use
PBOV1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~15.7 kDa | |
| Observed band | 72 kDa | |
| Gel | 15% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | Peptide-blocking control | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked PBOV1 Western Blot Protocol Options
The A15695 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | lysates from K562 cells, (catalog A15695) |
| Gel % | 15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.2 µm PVDF (standard starting point) |
| Blocking | the synthesized peptide (catalog A15695) |
| Primary antibody | A15695; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected PBOV1 Western Blot Band Size?
PBOV1 is predicted at 15.7 kDa, but antibody QC reports 72 kDa; the cause of the difference is not established.
| Band at 72 kDa | Reported in antibody QC; PBOV1 identity requires confirmation |
| Band near 15.7 kDa | Consistent with predicted PBOV1 mass if identity is confirmed |
| Band in cytoplasmic fraction | Consistent with reported cytoplasmic localization |
| Band in nuclear fraction | Consistent with reported nuclear localization |
💡Expected PBOV1 appearancePBOV1 is predicted at 15.7 kDa, while antibody QC reports a 72 kDa band; the difference is unexplained, so confirm band identity with peptide competition and an independent control.
| Predicted molecular weight | Sets a sequence-based reference near 15.7 kDa |
| Calculated mass in daltons | 15,722 Da corresponds to the 15.7 kDa prediction |
| Predicted mass of the 135-residue sequence | Provides the full-sequence size reference without an established migration shift |
| Predicted mass compared with the observed band | The 15.7 kDa prediction does not explain the empirical 72 kDa band |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | The sampled material may miss PBOV1 in the cytoplasm or nucleus | Check cytoplasmic and nuclear fractions with fraction controls |
| Band higher than expected | The reported 72 kDa band exceeds the predicted 15.7 kDa for an unknown reason | Compare peptide-blocked and unblocked lanes and confirm identity independently |
| Band lower than expected | The supplied features do not establish a smaller PBOV1 product | Check sample integrity and confirm band identity |
| Multiple bands | The supplied features do not establish multiple PBOV1 isoforms | Assess each band with peptide competition and an independent identity control |
| Weak or no signal | Sample recovery or antibody detection may be insufficient | Check loading and fraction recovery against a positive-control lysate |
Experiment evidence
Western blot images showing PBOV1 band
A15695 · PBOV1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PBOV1 Western blot
🧪For positive controls for PBOV1 in Western blot, you can use no HPA-supported tissue or cell sample because none is provided.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA data are unavailable, so tissue-based positive and negative controls cannot be selected.
HPA tissue expression evidence for PBOV1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced PBOV1 Western Blot Tips
Deeper troubleshooting and optimisation questions for PBOV1, answered from its protein features.
How should PBOV1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could a PBOV1 isoform explain a different band size?
Isoforms · The supplied record lists one isoform and no alternative sequence. It provides no isoform-based explanation for additional bands.
Do listed modifications explain a PBOV1 band shift?
PTM · The supplied record lists no modified residues or glycosylation sites. It therefore provides no modification-based explanation for a shift from the predicted 15.7 kDa mass. Investigate band identity before assigning a modification.
Does this guide establish induction of PBOV1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for PBOV1 Western blot?
Transfer · PBOV1 is predicted to be 15.7 kDa. Choose transfer conditions that retain small proteins, such as a 0.2 µm membrane, and check that the band transfers and remains on the membrane. The supplied features do not establish one required transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A15695 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should PBOV1 be quantified across cell fractions?
Quantitation · PBOV1 is reported in both the cytoplasm and nucleus. Quantify matched fractions separately, check fraction quality, and compare samples using appropriate loading normalization. A change in one fraction need not reflect a change in total PBOV1.
How should I interpret a 72 kDa band for PBOV1?
Interpretation · PBOV1 is predicted to be 15.7 kDa, so a 72 kDa band is substantially larger. The supplied UniProt features do not establish its identity or explain the difference. Verify the band with an independent PBOV1 antibody or a PBOV1 depletion control.
Compare each band's apparent mass with the predicted 15.7 kDa value. The supplied record lists no alternative sequence, oligomer, or modification that identifies another band. Use an independent antibody or PBOV1 depletion to test whether an unexpected band represents PBOV1.
Compare each band's apparent mass with the predicted 15.7 kDa value. The supplied record lists no alternative sequence, oligomer, or modification that identifies another band. Use an independent antibody or PBOV1 depletion to test whether an unexpected band represents PBOV1.
Boster reagents
PBOV1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-PBOV1 Antibody
Cat # A15695
A15695 is listed for human, mouse, and rat PBOV1. Its Western blot image uses K562 cell lysate and includes a peptide-blocked lane. The supplied evidence does not show mouse or rat Western blot samples.
Which to pick: A15695 is the only listed option. Choose it for the documented K562 Western blot context; human, mouse, and rat reactivity is listed, but the supplied image does not demonstrate mouse or rat samples.
Source: BosterBio PBOV1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q9GZY1.
- Human Protein Atlas. PBOV1 tissue expression.
- PMC5882516 — target-verified WB comparison