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- Table of Contents
PLAC8 / Placenta-specific gene 8 protein · Western blot design guide
Design a Western Blot for PLAC8
Source-linked PLAC8 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-PLAC8 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
PLAC8 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~12.5 kDa | |
| Observed band | Approximately 13 kDa | |
| Gel | 12% (catalog A11984) | |
| Positive control | Appendix (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked PLAC8 Western Blot Protocol Options
The A11984 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human THP-1 (catalog A11984) |
| Gel % | 12% (catalog A11984) |
| Load | 30 ug; reducing conditions (catalog A11984) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A11984) |
| Membrane | nitrocellulose membrane (catalog A11984) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A11984) |
| Primary antibody | A11984 · 0.5 μg/mL (catalog A11984) |
| Primary incubation | overnight at 4°C (catalog A11984) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A11984) |
| Secondary incubation | 1.5 hour at RT (catalog A11984) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A11984) |
| Detection | ECL (catalog A11984) |
Section 2
What Is the Expected PLAC8 Western Blot Band Size?
PLAC8 is predicted at 12.5 kDa and observed at approximately 13 kDa; the cause of the small difference is not established.
| Single band near 13 kDa | Matches the reported PLAC8 band; confirm identity with controls |
| Band near 12.5 kDa | Close to the UniProt predicted mass |
| Band well above 13 kDa | Identity or migration requires verification |
| Band well below 12.5 kDa | Identity or integrity requires verification |
💡Expected PLAC8 appearanceUniProt predicts PLAC8 at 12.5 kDa, and an antibody QC blot reports a band at approximately 13 kDa; confirm its identity with appropriate controls.
| UniProt predicted mass | Places the expected PLAC8 band near 12.5 kDa |
| Calculated mass of 12,507 Da | Corresponds to approximately 12.5 kDa |
| 115-residue sequence | Underlies the reported predicted mass |
| Predicted single-chain mass | Provides the reference for assessing apparent band size |
| Situation | Likely cause | Next action |
|---|---|---|
| Band higher than expected | The supplied features do not establish a size-increasing modification | Compare with a PLAC8 positive control and check antibody specificity |
| Band lower than expected | The supplied features do not establish a cleavage product | Check sample integrity and confirm identity with a PLAC8 control |
| Multiple bands | The single listed isoform does not explain distinct bands | Compare with a positive control and assess antibody specificity |
| Weak or no signal | PLAC8 may be below detection in the tested sample | Use a PLAC8 positive control and check loading and transfer |
| Fragments below expected size | Sample degradation is possible; no PLAC8 cleavage feature is listed | Check sample handling and compare with a fresh positive control |
Experiment evidence
Western blot images showing PLAC8 band
A11984 · PLAC8
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PLAC8 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for PLAC8 in Western blot, you can use appendix tissue lysate, which HPA scores High.
Positive control: Appendix (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA identifies both High and Not detected tissues, making tissue-based controls feasible.
HPA tissue expression evidence for PLAC8
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Appendix | enterocytes - Microvilli | High | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | High | Protein (IHC) | HPA → |
| Bronchus | ciliated cells (cilia axoneme) | High | Protein (IHC) | HPA → |
| Colon | enterocytes - Microvilli | High | Protein (IHC) | HPA → |
| Fallopian tube | ciliated cells (cilia axoneme) | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
| Caudate | glial cells | Not detected | Protein (IHC) | HPA → |
| Cerebellum | cells in granular layer | Not detected | Protein (IHC) | HPA → |
| Cerebral cortex | endothelial cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced PLAC8 Western Blot Tips
Deeper troubleshooting and optimisation questions for PLAC8, answered from its protein features.
How should PLAC8 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could another PLAC8 isoform explain a second band?
Isoforms · The supplied UniProt record lists one isoform and no alternative sequence. It provides no basis for assigning a second band to another PLAC8 isoform.
Do annotated modifications explain a PLAC8 band shift?
PTM · No modified residues or glycosylation sites are listed in the supplied record. Do not assign a shifted band to a specific modification from these features.
Does this guide establish induction of PLAC8?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for PLAC8 Western blot?
Transfer · PLAC8 is small: 115 residues with a predicted mass of 12.5 kDa. Choose transfer conditions that retain proteins in this size range, and check transfer near 13 kDa before interpreting a weak or absent band.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A11984 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
Which band should be quantified for PLAC8?
Quantitation · The reported apparent PLAC8 band is approximately 13 kDa, close to its 12.5 kDa predicted mass. Quantify the consistently identified band near that position; the supplied features do not identify other bands as PLAC8.
Why might PLAC8 appear near 13 kDa instead of 12.5 kDa?
Interpretation · The supplied predicted mass is 12.5 kDa, and the reported apparent band is approximately 13 kDa. That small difference alone does not establish a modification or processing event.
The supplied record lists no oligomer or disulfide annotation. A higher-mass band cannot be assigned to a PLAC8 oligomer from these features alone; establish its identity before including it in PLAC8 measurements.
The supplied record lists no oligomer or disulfide annotation. A higher-mass band cannot be assigned to a PLAC8 oligomer from these features alone; establish its identity before including it in PLAC8 measurements.
Boster reagents
PLAC8 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-PLAC8 Antibody Picoband®
Cat # A11984
The catalog reports one anti-PLAC8 antibody for Western blot: A11984, a rabbit polyclonal antibody with reported human reactivity. Its product image shows an approximately 13 kDa band in human THP-1 whole-cell lysate; the supplied evidence covers this tested context.
Which to pick: A11984 is the only listed option. It has a Western blot image using human THP-1 lysate, with 30 µg loaded under reducing conditions and antibody at 0.5 µg/mL. Reactivity is listed for human samples.
Source: BosterBio PLAC8 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q9NZF1.
- Human Protein Atlas. PLAC8 tissue expression.
- PMC10605972 — target-verified WB comparison
- PMC3498234 — target-verified WB comparison
- PMC5964121 — target-verified WB comparison