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- Table of Contents
PMS1 / PMS1 protein homolog 1 · Western blot design guide
Design a Western Blot for PMS1
Source-linked PMS1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-PMS1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
PMS1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~105.8 kDa | |
| Gel | 8–10% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 4 isoform(s) |
Section 1
Source-Linked PMS1 Western Blot Protocol Options
The A04425 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Hela, Raw264.7, PC12 (catalog A04425) |
| Gel % | 8–10% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Wet/tank transfer; optimize duration (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A04425; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected PMS1 Western Blot Band Size?
PMS1 is predicted at 105.8 kDa; no empirical band size is supplied, and migration effects from its four annotated isoforms are unestablished.
| Band near 105.8 kDa | Consistent with the predicted PMS1 mass; confirm band identity. |
| Several discrete bands | Could include splice isoforms 1, 2, 3, and 4; distinct migration is unestablished. |
| Band away from 105.8 kDa | An alternative isoform is possible, but its size is not supplied. |
| Nuclear-fraction band with weak whole-cell signal | Consistent with the annotated nuclear location. |
💡Expected PMS1 appearanceUniProt predicts PMS1 at 105.8 kDa; no empirical Western-blot band size is supplied, so confirm a band near that size with ordinary identity controls.
| UniProt predicted mass | Provides a 105.8 kDa reference, not a measured migration position. |
| Splice isoform 1 | Its apparent size relative to the other isoforms is unestablished. |
| Splice isoform 2 | Its apparent size relative to the other isoforms is unestablished. |
| Splice isoform 3 | Its apparent size relative to the other isoforms is unestablished. |
| Splice isoform 4 | Its apparent size relative to the other isoforms is unestablished. |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | PMS1 is annotated as nuclear. | Check a nuclear-enriched fraction and a positive-control lysate. |
| Band higher than expected | An isoform is possible, but its migration is unknown. | Confirm PMS1 identity with an independent antibody or PMS1 knockdown. |
| Band lower than expected | An isoform or nonspecific band is possible; isoform sizes are unknown. | Confirm PMS1 identity with an independent antibody or PMS1 knockdown. |
| Multiple bands | Four splice isoforms are annotated, but distinct bands are unproven. | Check which bands respond to PMS1 knockdown. |
| Weak or no signal | A nuclear protein may be diluted in whole-cell lysate. | Test a nuclear-enriched fraction and a positive-control lysate. |
Experiment evidence
Western blot images showing PMS1 band
A04425 · PMS1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PMS1 Western blot
🧪For positive controls for PMS1 in Western blot, you can use a PMS1-validated sample; the supplied HPA evidence identifies no positive tissue or cell line.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: Without HPA expression data, a positive sample needs independent validation and a knockdown or KO line can provide a negative control.
HPA tissue expression evidence for PMS1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced PMS1 Western Blot Tips
Deeper troubleshooting and optimisation questions for PMS1, answered from its protein features.
How should PMS1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Which PMS1 isoforms could produce smaller bands?
Isoforms · Four isoforms are listed. Relative to the canonical sequence, isoform 3 lacks residues 195–233; isoform 2 lacks 620–781; and isoform 4 lacks 1–176, 195–233, and 620–781. These deletions could change size, but the features do not establish which isoforms produce detectable bands.
Check whether the epitope lies within a deleted segment: 1–176 is absent from isoform 4, 195–233 from isoforms 3 and 4, and 620–781 from isoforms 2 and 4. These are UniProt canonical-sequence coordinates; antibody or paper numbering may differ.
Check whether the epitope lies within a deleted segment: 1–176 is absent from isoform 4, 195–233 from isoforms 3 and 4, and 620–781 from isoforms 2 and 4. These are UniProt canonical-sequence coordinates; antibody or paper numbering may differ.
Do annotated modifications prove a band shift?
PTM · The linked UniProt record describes protein features. A modification annotation alone does not demonstrate a visible shift; retain any condition or experimental qualifier attached to it.
Does this guide establish induction of PMS1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for PMS1 Western blot?
Transfer · PMS1 has a predicted mass of 105.8 kDa. Choose transfer conditions suitable for a protein of roughly 106 kDa and check transfer with a membrane stain. The supplied features do not identify a specific transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A04425 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should PMS1 bands be quantified across samples?
Quantitation · PMS1 is annotated as nuclear. Use a consistent sample preparation and compare bands detected with the same antibody. If the antibody recognizes multiple isoforms, define which bands are included in the measurement and apply that rule consistently.
How should the 105.8 kDa predicted mass guide band identification?
Interpretation · Use 105.8 kDa as a reference for canonical PMS1. No empirical band position is supplied, so a difference between apparent and predicted mass cannot be assigned to a specific cause from these features.
UniProt lists K619N in isoforms 2 and 4, alongside deletion of residues 620–781. The substitution alone does not establish a visible shift. Consider the listed deletions when assessing an isoform-dependent size difference.
Compare their positions with the listed isoform deletions and check whether the antibody epitope is retained. PMS1 also participates in the MLH1–PMS1 MutL-beta complex, but complex membership alone does not identify an unexpected band. The supplied features report no observed band positions.
UniProt lists K619N in isoforms 2 and 4, alongside deletion of residues 620–781. The substitution alone does not establish a visible shift. Consider the listed deletions when assessing an isoform-dependent size difference.
Compare their positions with the listed isoform deletions and check whether the antibody epitope is retained. PMS1 also participates in the MLH1–PMS1 MutL-beta complex, but complex membership alone does not identify an unexpected band. The supplied features report no observed band positions.
Boster reagents
PMS1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-PMS1 protein homolog 1 PMS1 Antibody
Cat # A04425
the supplier A04425 is an anti-PMS1 antibody listed for human, mouse, and rat. Its WB image shows HeLa, RAW264.7, and PC12 cell lysates at 1:500 dilution. The supplied evidence does not establish performance in other samples or conditions.
Which to pick: A04425 is the only listed PMS1 antibody. It has a WB image using HeLa, RAW264.7, and PC12 lysates at 1:500; choose it if those reported contexts and the listed human, mouse, or rat reactivity fit your experiment.
Source: BosterBio PMS1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P54277.
- Human Protein Atlas. PMS1 tissue expression.
- PMC11015039 — target-verified WB comparison
- PMC11989448 — target-verified WB comparison