JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.

DNA polymerase iota Antibodies

AND POLI ELISA kits, DNA polymerase iota Proteins

Many biological assays use antibodies to detect DNA polymerase iota, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with DNA polymerase iota in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop DNA polymerase iota antibodies...

We validate the specificity of these antibodies to DNA polymerase iota by testing them on tissues known to express POLI positively and negatively. Browse below to find the POLI antibody that suites your experiment. We have 6 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Previous: SEPTIN9 Next: FZD10

POLI Infographic by Boster Bio

POLI protein infographic

All POLI Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-DNA pol iota Antibody

Western blot analysis of the lysates from HeLa cells using POLI antibody

Western blot analysis of the lysates from HeLa cells using POLI antibody

Western Blot analysis of HeLa cells using DNA pol ι Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit

Human, Mouse

ELISA, WB

$399
Cat # A01376
100μl

Anti-DNA Polymerase iota/POLI Antibody Picoband®

7 Q&As

Western blot analysis of DNA Polymerase iota using anti-DNA Polymerase iota antibody (A01376-1)

Western blot analysis of DNA Polymerase iota using anti-DNA Polymerase iota antibody (A01376-1)

Western blot analysis of DNA Polymerase iota using anti-DNA Polymerase iota antibody (A01376-1)

Human, Mouse, Rat

WB

$370
Cat # A01376-1
100 μg/vial

Anti-DNA polymerase iota POLI Antibody

Western blot (WB) analysis of POLI polyclonal antibody at 1:500 dilution
Lane1:HEK293T whole cell lysate(40ug)
Lane2:A375 whole cell lysate(40ug)
Lane3:HCT116 whole cell lysate(40ug)
Lane4:SGC7901 whole cell lysate(40ug)

Western blot (WB) analysis of POLI polyclonal antibody at 1:500 dilution
Lane1:HEK293T whole cell lysate(40ug)
Lane2:A375 whole cell lysate(40ug)
Lane3:HCT116 whole cell lysate(40ug)
Lane4:SGC7901 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of POLI polyclonal antibody in paraffin-embedded human breast cancer tissue at 1:50

Human

IHC, WB

$405
Cat # A01376-2
100ul

DNA Polymerase iota (POLI) (NM_007195) Human Recombinant Protein

Coomassie blue staining of purified POLI protein

Coomassie blue staining of purified POLI protein

$1249
Cat # PROTQ9UNA4
20 µg

Anti-DNA Polymerase iota (POLI) Mouse Monoclonal Antibody [Clone ID: OTI1C8]

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLI (Right lane) cDNA for 48 hrs and lysed

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLI (Right lane) cDNA for 48 hrs and lysed

Western blot analysis of extracts (10ug) from 1 cell line by using anti-POLI monoclonal antibody (1:200)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-POLI mouse monoclonal antibody

Human

IHC, WB

$523
Cat # M01376
100 µl

DNA Polymerase iota (POLI) (NM_007195) Human Over-expression Lysate

DNA Polymerase iota (POLI) Human Over-expression Lysates NM_007195

DNA Polymerase iota (POLI) Human Over-expression Lysates NM_007195

Human

$960
Cat # LS011201
20 µg, 100 µg

POLI Overview

Facts about DNA polymerase iota.

POLI 3D structure

POLI 3D structure. Source: alphafold

DNA polymerase iota (POLI)

Error-prone DNA polymerase especially involved in DNA repair. Plays an essential role in translesion synthesis, in which the normal high-fidelity DNA polymerases can't move and DNA synthesis stalls.

Favors Hoogsteen base-pairing from the active website. Inserts the right base with high-fidelity reverse an adenosine template.

Gene Information

Human
Gene Name:	POLI
Uniprot:	Q9UNA4
Entrez:	11201

Gene Information

Family

Belongs to:

DNA polymerase type-Y family

Family

Alternative Names

DNA polymerase iota; EC 2.7.7.7; eta2; polymerase (DNA directed) iota; RAD30 homolog B; RAD30Bpolymerase (DNA-directed), iota; RAD3OB

Molecular Weight

Mass (kDA):
83.006 kDA

Molecular Weight

Genomic Context


Human

Location:	18q21.2
Sequence:	18; NC_000018.10 (54269479..54321266)

Tissue Specificity

Ubiquitous. Highly expressed in testis.

Subcellular Localizations

Nucleus. Binding to ubiquitin mediates localization to replication forks after UV-induced DNA damage.

Subcellular Localization

Diseases

Malignant Neoplasms
Neoplasms
Carcinogenesis
Adenoma
Carcinoma
Cell Transformation, Neoplastic
Malignant Squamous Cell Neoplasm
Immunologic Deficiency Syndromes
Influenza
Adenocarcinoma
Xeroderma
Xeroderma Pigmentosum
Inflammatory Response

Malignant Neoplasm Of Lung
Lung Adenoma
Pain
Physiological Stress
Inflammation
Nervousness
Lung Neoplasms

Disease

Interacting Proteins

DAZAP2
NEDD8
TMEM239
TRAF6
TRIM39

UBC
XRCC1
ZBTB44

Interacting Proteins

Pathways

Translesion Synthesis
Dna Repair
Fertilization
Photosynthesis
Inflammatory Response
Somatic Hypermutation Of Immunoglobulin Genes
Regeneration
Dna Replication
Lactate Oxidation
Strand Displacement
Mitochondrial Depolarization
Response To Heavy Metal
Double-strand Break Repair

Transpiration
Gene Conversion
Reverse Transcription
Fermentation
Translation
Cell Cycle
Mrna Processing

Pathways

PTMs

Deamination

Oxidation

Methylation

Research Areas

Chromatin Research
DNA Polymerases
DNA Repair

Research Areas

Bosterbio | ELISA Kits, Antibodies, Antibody Company

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/POLI

References

PMID: 10458907 by McDonald J.P., et al. Novel human and mouse homologs of Saccharomyces cerevisiae DNA polymerase eta.

PMID: 11013228 by Tissier A., et al. Misinsertion and bypass of thymine-thymine dimers by human DNA polymerase iota.